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微孔/MABE16 |抗磷酸化Sir2/SIRT1（Ser47）抗体，克隆EPR2849Y，兔单克隆/MABE16/100µ；L
微孔/MABE16 |抗磷酸化Sir2/SIRT1（Ser47）抗体，克隆EPR2849Y，兔单克隆/MABE16/100µ；L
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MABE16 |
| Description | Anti-phosphoSir2/SIRT1(Ser47)Antibody,cloneEPR2849Y,rabbitmonoclonal |
| AlternateNames |
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| BackgroundInformation | TheS.cerevisiaesilentinformationregulator2(Sir2)proteinisanNAD+-dependenthistonedeacetylase(HDACs)thatplaysacriticalroleintranscriptionalsilencing,genomestABIlity,andlongevity(1-2).PartoftheSirtuinfamily(Sir2,Sir3,Sir3),yeastSir2isthefoundingmemberofClassIIIHDACs.AhumanhomologueofSir2,SIRT1,regulatestheactivityofthep53tumorsuppressorandinhibitsapoptosis(3-4). |
| ProductInformation | |
|---|---|
| Format | Unpurified |
| Control |
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| Presentation | Rabbitmonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Phospho-specificpeptidecorrespondingtohumanSir2/SIRT1atSer47. |
| Epitope | Ser47 |
| Clone | EPR2849Y |
| Host | Rabbit |
| Specificity | ThisantibodydetectsSir2/SIRT1phosphorylatedonSer47. |
| Isotype | IgG |
| SpeciesReactivity |
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| SpeciesReactivityNote | Proventoreactwithhuman. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| Modifications |
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| PurificationMethod | Unpurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:NAD-dependentproteindeacetylase,whichregulatesprocessessuchasapoptosisandmuscledifferentiationbydeacetylatingkeyproteins.Deacetylates"Lys-382"ofp53/TP53andimpairsitsabilitytoinduceproapoptoticprogramandmodulatecellsenescence.DeacetylatesTAF1BandtherebyrepressesrDNAtranscriptionbytheRNApolymeraseI.InvolvedinHES1-andHEY2-mediatedtranscriptionalrepression.InhibitsskeletalmuscledifferentiationbydeacetylatingPCAFandMYOD1.MayserveasasensorofthecytosolicratioofNAD+/NADH,whichisessentialinskeletalmusclecelldifferentiation.Deacetylates"Lys-16"ofhistoneH4(invitro).ComponentoftheeNoSC(energy-dependentnucleolarsilencing)complex,acomplexthatmediatessilencingofrDNAinresponsetointracellularenergystatusandactsbyrecruitinghistone-modifyingenzymes.TheeNoSCcomplexisabletosensetheenergystatusofcell:uponglucosestarvation,elevationofNAD+/NADP+ratioactivatesSIRT1,leADIngtohistoneH3deacetylationfollowedbydimethylationofH3at"Lys-9"(H3K9me2)bySUV39H1andtheformationofsilentchromatinintherDNAlocus.IncaseofHIV-1infection,interactswithanddeacetylatestheviralTatprotein. CATALYTICACTIVITY:NAD++anacetylprotein=nicotinamide+O-acetyl-ADP-ribose+aprotein. COFACTOR:Binds1zincionpersubunitBysimilarity. ENZYMEREGULATION:Inhibitedbynicotinamide.Activatedbyresveratrol(3,5,4"-trihydroxy-trans-stilbene),butein(3,4,2",4"-tetrahydroxychalcone),piceatannol(3,5,3",4"-tetrahydroxy-trans-stilbene),Isoliquiritigenin(4,2",4"-trihydroxychalcone),fisetin(3,7,3",4"-tetrahydroxyflavone)andquercetin(3,5,7,3",4"-pentahydroxyflavone).RPS19BP1/AROSactsasapositiveregulatorofdeacetylationactivity.NegativelyregulatedbyKIAA1967/DBC1. SUBUNITSTRUCTURE:InteractswithTAF1B.FoundinacomplexwithPCAFandMYOD1Bysimilarity.ComponentoftheeNoSCcomplex,composedofSIRT1,SUV39H1andRRP8.InteractswithMLLT7/FOXO4,HES1,HEY2,p53/TP53andPML.InteractswithRPS19BP1/AROS.InteractswithKIAA1967/DBC1(viaN-terminus). SUBCELLULARLOCATION:Nucleus›PMLbody.Note:RecruitedtothenuclearbodiesviaitsinteractionwithPML. TISSUESPECIFICITY:Widelyexpressed. MISCELLANEOUS:Redwine,whichcontainsresveratrol,mayparticipateinactivationofsirtuinproteins,andmaythereforeparticipateinanextendedLifespanasithasbeenobservedinyeast. SEQUENCESIMILARITIES:Belongstothesirtuinfamily. Contains1deacetylasesirtuin-typedomain. SEQUENCECAUTIONS:ThesequenceAAH12499.1differsfromthatshown.Reason:Erroneousinitiation. |
| MolecularWeight | ~110kDaobserved |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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