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微孔/ES007-500ML | BCIP/TNBT单试剂，蓝色/棕色，碱性磷酸酶底物（不溶性）/ES007-500ML/500
微孔/ES007-500ML | BCIP/TNBT单试剂，蓝色/棕色，碱性磷酸酶底物（不溶性）/ES007-500ML/500
商品编号：
ES007-500ML
品牌：
密理博
市场价：
¥0.00
美元价：
0.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ES007-500ML |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | BCIP/TNBTSingleReagent,Blue/brown,AlkalinePhosphataseSubstrate(insoluble) |
| Overview | Althoughnitrobluetetrazoliumwithindoxylphosphateisconsideredanexcellentsubstrateforalkalinephosphataseimmunoblottingprocedures,theavailABIlityofhighlypuretetranitrobluetetrazolium(5-bromo-4-chloro-3-indoxylphosphate/tetranitrobluetetrazolium)(TNBT)maynowallowtheimmunologistandmolecularBIOLOGisttoachieveevengreatersensitivity.ChemiconInternationalhasmadeavailablearefrigerator-stable,singlecomponentBCIP/TNBTsolutionforWestern,Northern,andSoutherntechniquesaswellasimmunohistochemicalandinsituhybridizationapplications.ThereactionproductobservedisadarkerpurplethanthatobservedwithNBTandmaybeusefulfordetectionofmultiplealkalinephosphataseprobeswhenusedinconjunctionwithBCIP/NBT.MethodologyandreactiontimesaresimilartotheBCIP/NBTsystem,butmaybeshortenedbecauseofincreasedsensitivity. NOTE:DONOTUSEPHOSPHATEBUFFERSFORWASHING.InorganicphosphateisapowerfulinhibitorofAP. |
| ProductInformation | |
|---|---|
| Presentation | BCIP/TNBTinaproprietarybufferwithenhancer. |
| ColorCode | Black |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainrefrigeratedat2-8°Cforupto12months.Warmtoroomtemperaturepriortouse.Discardifsolutionisturbidorpurple. |
| Applications | |
|---|---|
| Application | 500mlBCIP/TNBTSingleReagent,Blue/brown,AlkalinePhosphataseSubstrate(insoluble)forWesternBlotting&IHC. |
| KeyApplications |
|
| ApplicationNotes | PROCEDUREFORBCIP/TNBT(ES007): 1)AfterthefinalbindingreactionwithanAPlabeledprobe,washtissuesectionsormembranethoroughlyinabuffersuchasTris/HClorTris-bufferedsalinecontaining0.1%Tween20.DONOTUSEPHOSPHATEBUFFERS.InorganicphosphateisapowerfulinhibitorofAP. 2)AfterthefinalwashcompletelycovertissuesectionsormembranewithBCIP/TNBTSolutionandincubate5-15minutesprotectedfromlight.Longerincubationsmaybenecessarydependinguponenzymeactivity.Ifcolordevelopsalmostimmediately,theformazandepositsmayflakeoffofthetissuesectionsormembrane.Ifthisoccurs,furtherdilutionoftheAPprobeisnecessary.Afinelineofformazandepositcircumscribingthebandordot,withnodepositinthecenter,alsosuggestsfurtherdilutionoftheAPprobestobenecessary. 3)Stoptheenzymereactionbythoroughlywashingtissuesectionsormembraneswithdeionizedwater. 4)Ifdesired,counterstaintissuesectionsina0.5%NeutralRedsolutionpreparedinaMolL(-1)acetatebuffer.Hematoxylinsolutionswillnotprovideappropriatecontrast.Membranesshouldbedriedandstoredprotectedfromlight.Littleornobackgroundstainingshouldbeobservedonsectionsormembranes.Presenceofexcessivebackgroundstainingindicatesincompleteremovalofnon-boundAPfromthesectionsormembrane.Increasethenumberofwashesorthewashingtimetoremedythisproblem. Results: AfinedarkpurpleprecipitatewilllocalizeatsitesofAPactivityontissuesections.DarkpurplebandsordotswillbevisIBLeatthesitesofAPactivityonmembranes. |
| BiologicalInformation |
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| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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