当前位置：
首页
>
产品中心 >
Sandwich_method_ELISA >
Millipore/ES001-500ML | TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate (soluble)/ES001-500ML/500 mL
Millipore/ES001-500ML | TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate (soluble)/ES001-500ML/500 mL
商品编号：
ES001-500ML
品牌：
密理博
市场价：
¥2760.00
美元价：
1656.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ES001-500ML |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | TMB/ESingleReagent,Bluecolor,HorserADIshPeroxidaseSubstrate(soluble) |
| Overview | TMB,(3,3",5,5"-tetramethybenzidine)hasbeenshowntobeasafe-sensitivesubstratefortheassayofHRP.Initially,inthepresenceofHRPandhydrogenperoxide,aone-electronoxidationproductisformed.Thiscompound,acationfreeradical,isblueincolorwithanadsorptionmaximumof653nm.FurtherreactionwithHRP/H2O2oracidificationoftheradicalwithacidyieldsthediimineterminaloxidationproductadsorbinglightat450nm.Theextinctioncoefficientoftheradical(E653nm=3.9x104mol-1cm-1)anddiimine(E450nm=5.9x104mol-1cm-1)providearemarkablysensitivesystemfortheassayofHRPandHRPlabeledprobes.ES001isasinglecomponentreagentstableatroomtemperatureandnotsensitivetonormallaboratorylight.ItisoptimizedwithrespecttoTMBandhydrogenperoxideconcentrationsandyieldsalinearresponsewiththeconcentrationsofHRPusuallyemployedinimmunologicalassays. |
| ProductInformation | |
|---|---|
| Presentation | REAGENTPROVIDED: LiquidsolutioncontainingTMB,1.25mMol/LandHydrogenPeroxide,2.21mMol/Landlessthan1%dimethylsulfoxideina0.08Mol/L)acetatebufferatpH4.9.Alsocontainsnon-toxicproprietarystABIlizers. MaterialsRequired: ReagentsRequiredforStoppingtheReaction,ButNotProvided A.Sodiumfluoride,0.1%forstoppingthereactionandpreservingbluechromogen.Preparebydissolving1gofsodiumfluoridein1literofreagentgradewater.Observeallprecautionsonlabelforuseofsodiumfluoride. B.Acidforstoppingthereactionandconvertingtheblueradicaltotheyellowdiimine.Either0.5Mol/Lsulfuricacidor0.25Mol/Lhydrochloricacidmaybeused.THEABSORBANCEMUSTBEOBTAINEDWITHIN5MINUTESAFTERADDINGTHEACID. Note:Reagentgradewatermustcontainlessthan10-7Mol/LofironorcoppersaltsotherwiseunreactedTMBwillbeconvertednon-enzymaticallytothediimine. |
| ColorCode | Yellow |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainatroomtemperatureforuptosixmonths.Forlongtermstorage,uptotwelvemonths,maintainat2-8°C.Protectfromexposuretodirectsunlight.Discardifsolutionisblueorturbid. |
| Applications | |
|---|---|
| Application | 500mlTMB/ESingleReagent,Bluecolor,HorseradishPeroxidaseSubstrate(soluble)forELISA. |
| KeyApplications |
|
| ApplicationNotes | METHODSYNOPSIS: AftercompletionofanalytebindingtoasolidphaseandreactionwithaHRPlabeledprobe,ES001isadded.OxidationofTMBproducesabluereactionproductthatismeasuredat650nm.Thecolorformationasafunctionoftimecanberecordedorthereactionstoppedwithsodiumfluorideafterafixedinterval.Increasedsensitivitycanbeachievedbyconvertingtheblueradicaltothediiminebyadditionofacid.Theresultingyellowcolorismeasuredimmediatelyat450nm. SAMPLEPROCEDURE 1.Completeallrequiredincubationswithantibodies,probes,HRPlabeledreagents. 2.Washplateatleast4timeswithPBSorTrisbufferedsalinecontaining0.1%Tween-20. 3.Afterthefinalwash,shakeandblotallresidualbufferfromplatewells. 4.Add0.1mLofES001(TMBESolution)toappropriatewellsandincubate5-30minutes. Note:ThereactiontimewilldependupontheactivityoftheHRPprobe.Ifcolordevelopstoobriskly,zeroorderkineticswillnotprevail.Dilutionoftheprobe,antibodyorHRPlabeledreagentmayberequired. 5.Thereactioncanbemonitoredasafunctionoftimeforkineticassaysorstoppedwith0.1mLof0.1%sodiumfluorideandreadat650nm. 6.Iftheproceduredemandsconversiontotheyellowdiimine,add0.1mLofeitheraciddescribedinthereagentsectionaboveandrecordtheabsorbancewithin5minutes. Variationsoftime,reagentvolumeandtemperaturerequirestandardizationbytheuser. |
| BIOLOGicalInformation |
|---|
| PhysicochemicalInformation | |
|---|---|
| QuantitationWavelength | 450nm |
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
