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Millipore/15-103 | PI3 Kinase/Akt/GSK3 Pathway Explorer Minipack/15-103/3 vials/Pk
Millipore/15-103 | PI3 Kinase/Akt/GSK3 Pathway Explorer Minipack/15-103/3 vials/Pk
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¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 15-103 |
| BrandFamily | Upstate |
| TradeName |
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| Description | PI3Kinase/Akt/GSK3PathwayExplorerMinipack |
| AlternateNames |
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| BackgroundInformation | PathwayExplorerAntibodyMiniPack: EachPathwayExplorerAntibodyMinipackcontainsthreerelatedantibodiesaspartofasignalingcascadeoracombinationoftotalandphosphorylatedformsofkeysignalingtargets.Eachofthethreeantibodiesare30%theoriginalpacksize.FullsizeversionsofeachofthePathwayExplorerantibodiesareavailableforsaleindividuallyunderthesamecatalognumberwiththeremovalof“SP”offofeachone(e.g.05-591SPcanbeorderedas05-591). PI3Kinase: PI3Kinase(Phosphatidylinositol3-Kinase)isresponsIBLeforphosphorylationofthe3positionoftheinositolringofPI(4,5)P2,togeneratePI(3,4,5)P3,apotentsecondmessengerrequiredforsurvivalsignaling,andinsulinaction.PI3Kinaseisaheterodimericcomplexcomposedofan85kDaregulatorysubunitanda110kDacatalyticsubunit.Tyrosinephosphorylationofgrowthfactorreceptorscreatesdockingsitesforbindingofp85(throughitsSH2domains)onthereceptors;p85bringswithitp110,whichisthenproximaltoitsphospho-lipidsubstrateonthemembrane.PI3KinaseisalsoactivatedbyRas,andbytheβ-γsubunitsofheterotrimericG-proteins.PI3Kinaseisinhibitablebywortmannin,ausefultoolforthestudyofthePI3Kinasesignalingpathway. Akt/PKB,PHDomain: Akt(proteinkinaseB),aserine/threoninekinase,hasemergedasacriticalenzymeinsignaltransductionpathwaysinvolvedincellproliferation,apoptosis,angiogenesis,anddiabetes.InmammalsthreeisoformsofAkt(α,β,γorAkt1,2,3)arereportedthatexhibitahighdegreeofhomology,butdifferslightlyinthelocalizationoftheirregulatoryphosphorylationsites.Aktαisthepredominantisoforminmosttissues,whereasthehighestexpressionofAktβisobservedintheinsulin-responsivetissues,andAktγisabundantinbraintissue.EachAktisoformiscomposedofthreefunctionallydistinctregions:anN-terminalpleckstrinhomology(PH)domainthatprovidesalipid-bindingmoduletodirectAkttoPIP3,acentralcatalyticdomain,andaC-terminalhydrophobicmotif. GSK3α/β: Glycogensynthasekinase-3(GSK-3),amultifunctionalserine/threoninekinase,isakeyregulatorofnumeroussignalingpathways.TwoisoformsofGSK-3arereportedinmammals:a51kDaGSK-3αanda47kDaGSK-3β.Thesetwoisoformsexhibitabout98%homologyintheirkinasedomains,butshareonlyabout36%identityinthelast76C-terminalaminoacidresidues.GSK-3αcontainsaglycine-richextensionatitsN-terminus.Aminor(~15%oftotal)splicevariantofGSK-3β,GSK-3β2,hasalsobeenidentified,whichcontainsa13-residueinsertwithinthekinasedomain.ItexhibitsreducedkinaseactivitytowardstheTauproteincomparedwith‘unspliced’GSK-3β.GSK-3β2islocalizedprimarilytoneuronalcellbodies,unlikeunsplicedGSK-3βthatisalsofoundinneuronalprocesses.GSK-3βplaysakeyinhibitoryroleintheWntsignalingpathway.Wntgenesencodealargefamilyofsecreted,cysteine-richproteinsthatareimportantindevelopmentandinmaintenanceofadulttissues.AbnormalitiesinWntsignalingarereportedtopromotebothhumandegenerativediseasesandcancer. *Seefullsizeversionsforcorrespondingreferences. |
| ProductInformation | |
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| Components |
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| Control |
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| Presentation | 3individualtubescontainingeitherAnti-PI3Kinase,p85;Anti-Akt/PKB,PHDomain,cloneSKB1;orAnti-GSK3α/β,clone4G-1E |
| Properties | Eachvialis30%thesizeoftheparentcatalognumber |
| StorageandShippingInformation |
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| Applications | |
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| Application | ThisAntibodypackcontainsAnti-PI3KinaseAntibody,Anti-Akt/PKBAntibody,Anti-GSK3α/βAntibodyvalidatedforuseinEnzymeAssay,ICC,IF,Flow,IP&WB. |
| KeyApplications |
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| ApplicationsNotRecommended |
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| ApplicationNotes | 05-412SP:ImmunofluorescenceStaining: L6cellsweregrown,fixed,andpermeABIlized.; FlowCytometry: NCI/H292cellswerestainedwithAnti-GSK3α/β,clone4G-1EconjugatedtoAlexaFluor®555;Immunoprecipitation:Notrecommendedfor05-412SP. 06-195SP:Immunoprecipitation:5µLofapreviouslotimmunoprecipitatedPI3kinasefromhumanJurkatcelllysateasconfirmedbysubsequentwesternblot.Theantibodywillprecipitatethe85kDasubunit. 05-591SP:ImmunofluroescenceAnalysis: A431cellswerefixed,permeabilized,andstainedwithanti-Akt,cloneSKB1(2μg/mL)conjugatedtoAlexaFluor®488; FlowCytometry:NIH/3T3cells(detachedusingtrypsin-EDTA)persamplewerestainedwith0.5μgofanti-Akt/PKB,PHDomain,cloneSKB1(filledhistogram)or1μgofmouseIgG1(openhistogram)followedbyagoatanti-mouseIgG-PEconjugate. ImmunoprecipitationKinase:4μgofapreviouslotimmunoprecipitatedactiveAktfrom700μgofL6cellsstimulatedwith15nMIGF-1for2-5minutes. ImmunoprecipitationKinaseAssayData: ThekinaseactivityofimmunoprecipitatedAkt/PKBwasdeterminedasdescribedaboveusingAkt/SGKSubstratePeptideasasubstrate.Activekinasewasdetectedbycomparingtheincorporationof[32P]intoAkt/SGKSubstratePeptideascatalyzedbyimmunoprecipitates(1mg)ofL6celllysatesstimulatedwith15nMIGF-1.Thetestresultsareshowninthetabletotheleft. |
| BIOLOGicalInformation | |
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| Immunogen | 05-412SP:18residuesyntheticpeptide(C-KQLLHGEPNVSYICSRY)correspondingtoaminoacids203-219derivedfromaregionofthecatalyticdomain(betweensuBDomainVIIandVIII)oftheDrosophilaGSK3/shaggyenzyme,coupledtoovalbumin.Clone4G-1E.;05-591SP:GST-fusionproteincorrespondingtoresidues1-149ofhumanAkt1.;06-195SP:GSTfusionproteinscontaining1)theN-terminalSH2domainofratPI3kinaseand2)thefulllength85kDasubunitofratPI3kinase. |
| Epitope | Varies.SeeindividualDataSheets |
| Clone | 05-412SP:4G-1E;05-591SP:SKB1 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| SpeciesReactivity |
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| SpeciesReactivityNote | 05-412SP:Human,Mouse,Rat,S.pombe,andDictyostelium.Becauseoftheextremelyhighconservationofthisimmunogenamongtheknownenzymesofmanydifferentspecies,thisantibodyispredictedtocross-reactwithnearlyallspecieshomologues. 05-591SP:Human,mouseandrat;otherspeciescross-reactivityunknown. |
| EntrezGeneNumber | |
| EntrezGeneSummary | AKT1:Theserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene.[providedbyRefSeq]. P13KINASE:Phosphatidylinositol3-kinasephosphorylatestheinositolringofphosphatidylinositolatthe3-primeposition.Theenzymecomprisesa110kDcatalyticsubunitandaregulatorysubunitofeither85,55,or50kD.Thisgeneencodesthe85kDregulatorysubunit.Phosphatidylinositol3-kinaseplaysanimportantroleinthemetabolicactionsofinsulin,andamutationinthisgenehasbeenassociatedwithinsulinresistance.Alternativesplicingofthisgeneresultsinthreetranscriptvariantsencodingdifferentisoforms.[providedbyRefSeq]. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | PI3Kinase,p85: FUNCTION:Bindstoactivated(phosphorylated)protein-Tyrkinases,throughitsSH2domain,andactsasanadapter,mediatingtheassociationofthep110catalyticunittotheplasmamembrane.Necessaryfortheinsulin-stimulatedincreaseinglucoseuptakeandglycogensynthesisininsulin-sensitivetissues. Subunitstructure:Heterodimerofap110(catalytic)andap85(regulatory)subunits.InteractswithphosphorylatedTOM1L1.InteractswithphosphorylatedLIME1uponTCRand/orBCRactivation.InteractswithSOCS7.InteractswithRUFY3Bysimilarity.InteractswithphosphorylatedLAT,LAX1andTRAT1uponTCRactivation.InteractswithCBLB.InteractswithHIV-1NeftoactivatetheNefassociatedp21-activatedkinase(PAK).ThisinteractiondependsontheC-terminusofbothproteinsandleadstoincreasedproductionofHIV.InteractswithHCVNS5A.TheSH2domainsinteractwiththeYTHMmotifofphosphorylatedINSRinvitro.Alsointeractswithtyrosine-phosphorylatedIGF1Rinvitro.InteractswithCD28andCD3ZuponT-cellactivation.InteractswithIRS1andphosphorylatedIRS4,aswellaswithNISCHandHCST. TISSUESPECIFICITY:Isoform2isexpressedinskeletalmuscleandbrain,andatlowerlevelsinkidneyandcardiacmuscle.Isoform2andisoform4arepresentinskeletalmuscle(atproteinlevel). DOMAIN:TheSH3domainmediatesthebindingtoCBLB,andtoHIV-1Nef. Post-translationalmodification:PolyubiquitinatedinT-cellsbyCBLB;whichdoesnotpromoteproteasomaldegradationbutimpairsassociationwithCD28andCD3ZuponT-cellactivation. AKT: FUNCTION:Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI(3)K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesis,partlybyplayingaroleinbothinsulin-inducedphosphorylationof4E-BP1andininsulin-inducedactivationofp70S6kinase.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase. CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein. ENZYMEREGULATION:Threespecificsites,oneinthekinasedomain(Thr-308)andthetwootheronesintheC-terminalregulatoryregion(Ser-473andTyr-474),needtobephosphorylatedforitsfullactivation. SUBUNIT:InteractswithCENTG1isoform2(PIKE-A)inthepresenceofguaninenucleotides.TheC-terminusinteractswithCCDC88A/GRDNandTHEM4.InteractswithAKTIP.Interacts(viaPHdomain)withMTCP1,TCL1AANDTCL1B.InteractswithCDKN1B;theinteractionphosphorylatesCDKN1Bpromoting14-3-3bindingandcell-cycleprogression. SUBCELLULARLOCATION:Cytoplasm.Nucleus.Cellmembrane.Note=Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1).NucleartranslocationisenhancedbyinteractionwithTCL1A. TISSUESPECIFICITY:Inallhumancelltypessofaranalyzed. DOMAIN:BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI(3)K)resultsinitstargetingtotheplasmamembrane. DOMAIN:TheAGC-kinaseC-terminalmediatesinteractionwithTHEM4. PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.Ser-473phosphorylationbytheRictor-mTorcomplexfavorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithCENTG1isoform2(PIKE-A).Ser-473phosphorylationisenhancedinfocalcorticaldysplasiaswithTaylor-typeballooncells. GSK3: FUNCTION:ParticipatesintheWntsignalingpathway.Implicatedinthehormonalcontrolofseveralregulatoryproteinsincludingglycogensynthase,MYBandthetranscriptionfactorJUN.PhosphorylatesJUNatsitesproximaltoitsDNA-bindingdomain,therebyreducingitsaffinityforDNA.PhosphorylatesMUC1inbreastcancercells,anddecreasestheinteractionofMUC1withCTNNB1/beta-catenin. CATALYTICACTIVITY:ATP+[tauprotein]=ADP+[tauprotein]phosphate. ENZYMEREGULATION:InhibitedwhenphosphorylatedbyAKT1. SUBUNIT:MonomerBysimilarity.InteractswithCABYR,MUC1,NINandPRUNE. TISSUESPECIFICITY:Expressedintestis,thymus,prostateandovaryandweaklyexpressedinlung,brainandkidney. POST-TRANSLATIONALMODIFICATION:PhosphorylatedbyAKT1andILK1. SEQUENCESIMILARITIES:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.GSK-3subfamily.Contains1proteinkinasedomain. |
| MolecularWeight | 05-412SP:51kDa(α)or46kDa(β);05-591SP:60kDa;06-195SP:85kDa |
| PhysicochemicalInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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