密理博/ST1013 |磷酸盐™ 抗GSK-3α/β(pTyr279/216)兔pAb/ST1013/
市场价:
¥9460.00
美元价:
5676.00
产品分类:
功能性抗体
公司分类:
Functional_antibody
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description | |
---|---|
Overview | Recognizesthe~51kDaGSK-3αproteinphosphorylatedatTyr279andthe~47kDaproteinGSK-3βphosphorylatedatTyr216. |
Note:1T=1test.Sufficientfor10WesternminIBLots. | |
CatalogueNumber | ST1013 |
BrandFamily | Calbiochem® |
Synonyms | Anti-GlycogenSynthaseKinase-3α/β,Phospho-Specific(Tyr279/216) |
ApplicationData | DetectionofhumanGSK-3β,phosphorylatedatTyr216byimmunoblotting.Samples:Wholecelllysatefromcontrolcells(lane1)orcontrollysatespikedwithhumanrecombinantGSK-3β(lanes2-5).Primaryantibody:PhosphoDetect™Anti-GlycogenSynthaseKinase-3α/β(pTyr279/216)RabbitpAb(Cat.No.ST1013)(1:1000)pre-incubatedwithoutpeptide(lanes1,5),orwithphosphopeptideimmunogen(lane2),agenericphosphoserine-containingpeptide(lane3),ornon-phosphopeptidecorrespondingtotheimmunogen(lane4).Detection:chemiluminescence. |
ProductInformation | |
---|---|
Form | Liquid |
Formulation | InDulbecco"sPBS(withoutMg2+andCa2+),50%glycerol,1mg/mlBSA,pH7.3. |
Negativecontrol | Normalrabbitserum |
Positivecontrol | Serumstarvedinsulin-treated3T3-L1cells |
Preservative | ≤0.1%sodiumazide |
StorageandShippingInformation | |
---|---|
ShipCode | BlueIceOnly |
Toxicity | StandardHandling |
Storage | -20°C |
Avoidfreeze/thaw | Avoidfreeze/thaw |
Donotfreeze | Oktofreeze |
SpecialInstructions | Followinginitialthaw,aliquotandfreeze(-20°C). |
Applications | |
---|---|
KeyApplications | Immunocytochemistry DotBlot Enzyme-LinkedImmunosorbentAssay Immunoblotting(WesternBlotting) |
ApplicationNotes | DotBlot(seecomments) ELISA(seecoments) Immunoblotting(1:1000) Immunocytochemistry(1:400) |
ApplicationComments | Togenerateapositivecontrol,serumstarve3T3-L1cellsfor3h,thenstimulatewith100nMinsulinor100ng/mlIGF-1for5min.ThisantibodyhasalsobeenreportedtoworkforELISAanddotblots.Ata1:1000dilutionthereisenoughantibodyfor10immunoblotsat10ml/blot.Antibodymustbetitratedforoptimalresultsinindividualsystems. |
BIOLOGicalInformation | |
---|---|
Immunogen | asyntheticphosphopeptidecorrespondingtoaminoacidssurroundingtheTyr279/216phosphorylationsitesofhumanGSK-3α/β |
Immunogen | Human |
Host | Rabbit |
Isotype | IgG |
SpeciesReactivity |
|
AntibodyType | PolyclonalAntibody |
PhysicochemicalInformation |
---|
Dimensions |
---|
MaterialsInformation |
---|
MaterialsInformation |
---|
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。
联络我们