当前位置：
首页
>
产品中心 >
Functional_antibody >
Millipore/MAB3572 |抗肌球蛋白抗体，平滑肌重链，SM1和SM2，克隆V/10/MAB3572/100和micro；G
Millipore/MAB3572 |抗肌球蛋白抗体，平滑肌重链，SM1和SM2，克隆V/10/MAB3572/100和micro；G
市场价：
¥6160.00
美元价：
3696.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB3572 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-MyosinAntibody,smoothmuscleheavychain,SM1&SM2,cloneV/10 |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Liquidin0.02MPhosphatebufferwith0.25MNaCland0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat2-8*Cinundilutedaliquotsupto6monthsafterdateofreceipt |
| Applications | |
|---|---|
| Application | Anti-MyosinAntibody,smoothmuscleheavychain,SM1&SM2,cloneV/10detectslevelofMyosin&hasbeenpublished&validatedforuseinIP,WB,IC,IH(P). |
| KeyApplications |
|
| ApplicationNotes | Westernblot(seeapplicationnotesbelow).SuggestedblockingbufferisTBS-Tweenwith2%BSA.SuggesteddilutionbufferisTBS-Tweenwith0.05%sodiumazide.Preferredgelpercentageis5%(seeapplicationnotes). Immunohistochemistryonfrozenandparaffinembeddedtissuesections.Suggestedfixationforfrozentissuesectionsisacetonefixfor6minutesatroomtemperature.Forformalinfixedparaffinembeddedtissuesections:microwavein0.01Mcitratebuffer(pH6.0)for8-10minutes(notethatallmicrowavesdifferandadjustmentsmayneedtobemade)followwithenzymedigestion(0.01%pronasefor10mintues).Suggestedblockingagentisfetalbovineserum.Theantibodyhasalsobeenusedsuccessfullyonmethyl-Carnoyfixedtissue. Immunocytochemistry Immunoprecipitation.Suggestedextractionbufferis20mMTris-HCl,pH7.4,150mMNaCl,1%TritonX-100,0.1%SDS,0.5%deoxycholicacid-NaCland0.5mMPMSF.Finalreactionvolumeis1mLandsuggestedcaptureagentisagaroseconjugatedanti-mouseIgG. Optimalworkingdilutionsmustbedeterminedbytheenduser. APPLICATIONNOTESFORMAB3572 WESTERNBLOT Toachievegoodresolutionofmyosinheavychainisoformswithdistinctmolecularweight(200-2004kDa),thefollowingprocedureshouldbefollowed:1).Pyrophoshateextractionbufferforsamplepreparation(seebelow);runSDS-PAGEin5%gel.Important:forbetterresolutionoftheMHCbands,useelectrophoreticbufferwithpH8.2(i.e.0.1lessthanstandard),andprepareresolvinggel(5%)withpH9.0(not8.8asusual).Alsohelpthoroughdegasingoftheresolvinggelmixture(H2O,acrylamide,EDTA,pH9.0,before(!)addingSDS,TEMEDandAPS).RunSDS-PAGElongerthanafterthedyefrontrunsoff(use200kDaMWMarkersandletit"s200kDabandrunatleasttothemiddleof8X8gel(usingabigsizegel(notthemini-gel!)willenhancethequalityofMHCbandresolution). Pyrophosphateextractionbuffer:(40mMNa4P2O7x10H2O,1mMMgCl2,1mMEGTA(addKOHtodissolveEGTA),PMSF,pH9.5).Toextractacto-myosinfromtissues/cells,shakemincedtissueorcellsincoldextractionbuffer1hronicebath(0oC),centrifuge@10,000gfor10minat+2-8oC,takesupernatantandmixit1:1withstandardLaemmlisamplebuffer,boil,runSDS-PAGEin5%gel(seeabove). |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Crudesmoothmuscleextractfromnormalhumanadultuterus. |
| Epitope | smoothmuscleheavychain,SM1&SM2 |
| Clone | V/10(alsoreferredtoasSM-M10) |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Smoothmusclemyosin,heavychain.ByWesternblottheantibodyrecognizesaproteinof200-204kDa. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
|
| UniProtNumber | |
| UniProtSummary | SIZE:780aminoacids;88314Da |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
