Millipore/ABE208 | Anti-β-Catenin Antibody/ABE208/100 µg
市场价：
¥6160.00
美元价：
3696.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ABE208 |
| Replaces | 04-1002 |
| Description | Anti-β-CateninAntibody |
| AlternateNames |
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| BackgroundInformation | β-cateninisthefoundingmemberoftheβ-cateninfamily.Thisproteiniscomprisedof12ARMrepeatsandisconsideredtohavedualfunctiondependingonitslocalization.Cytoplasmicβ-cateninisacriticalcomponentofE-cadherin/cateningadhesioncomplexesandisinvolvedinregulatingcadherin-dependentcell-cellinteractions.Whenlocalizedtothenucleus,β-cateninservesaregulatoryrolebybindingwithtranscriptionfactorsfromtheTCF/LEFfamily,activatingtranscription.Expressionofβ-cateninhasbeenreportedinavarietyofhairfolliclecellssuchascellsfoundintheinnerandouterportionsoftherootsheaths,andthebasalandperipheralmatrixcells.Ithasalsobeenobservedincolontissue.Defectsinexpressionhavebeenlinkedtothedevelopmentofseveraltumorsandcancerssuchas;colorectalcancer(CRC),medulloblastoma(MDB),ovariancancer(OC),andpilomatrixoma(PTR). |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Control |
|
| Presentation | Purifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugatedlinearpeptidecorrespondingtotheconsensusGSK3phosphorylationsiteofhumanβ-Catenin. |
| Epitope | ConsensusGSK3phosphorylationsiteofhumanβ-Catenin |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | Antibodyrecognizesβ-CateninattheN-terminus. |
| SpeciesReactivity |
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| SpeciesReactivityNote | Demonstratedtoreactwithhuman,mouse,andrat.Predictedtoreactwithequineandoxbasedon100%sequencehomology. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Theproteinencodedbythisgeneispartofacomplexofproteinsthatconstituteadherensjunctions(AJs).AJsarenecessaryforthecreationandmaintenanceofepithelialcelllayersbyregulatingcellgrowthandadhesionbetweencells.TheencodedproteinalsoanchorstheactinCytoskeletonandmayberesponsIBLefortransmittingthecontactinhibitionsignalthatcausescellstostopdividingoncetheepithelialsheetiscomplete.Finally,thisproteinbindstotheproductoftheAPCgene,whichismutatedinadenomatouspolyposisofthecolon.Mutationsinthisgeneareacauseofcolorectalcancer(CRC),pilomatrixoma(PTR),medulloblastoma(MDB),andovariancancer.Threetranscriptvariantsencodingthesameproteinhavebeenfoundforthisgene. |
| GeneSymbol |
|
| PurificationMethod | AffinityPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:InvolvedintheregulationofcelladhesionandinsignaltransductionthroughtheWntpathway. SIZE:781aminoacids;85497Da SUBUNIT:Twoseparatepoolsarefoundinthecytoplasm:oneisPSEN1/cadherin/catenincomplexwhichanchorstotheactincytoskeleton.TheotherpoolispartofalargecomplexcontainingAXIN1,AXIN2,APC,CSNK1A1andGSK3BthatpromotesphosphorylationonN-terminalSerandThrresiduesandubiquitinationofCTNNB1viaBTRCanditssubsequentdegradationbytheproteasome.Wnt-dependentactivationofDVLantagonizestheactionofGSK3B.WhenGSK3Bactivityisinhibitedthecomplexdissociates,CTNNB1isdephosphorylatedandisnolongertargetedfordestruction.ThestABIlizedproteintranslocatestothenucleus,whereitbindsTCF/LEF-1familymembers,TBP,BCL9andpossiblyalsoRUVBL1andDUPLIN.BindsCTNNBIPandEP300.CTNNB1formsaternarycomplexwithLEF1andEP300thatisdisruptedbyCTNNBIP1binding(Bysimilarity).InteractswiththePDZdomainofTAX1BP3;thisinteractioninhibitsthetranscriptionalactivityofCTNNB1(Bysimilarity).InteractswithAJAP1,BAIAP1,CARM1,CTNNA3,CXADRandPCDH11Y.BindsSLC9A3R1.InteractswithGLIS2.InteractswithSLC30A9(Bysimilarity). SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Cytoplasmicwhenitisunstabilized(highlevelofphosphorylation)orboundtoCDH1.Translocatestothenucleuswhenitisstabilized(lowlevelofphosphorylation).InteractionwithGLIS2promotesnucleartranslocation. TISSUESPECIFICITY:Expressedinseveralhairfolliclecelltypes:basalandperipheralmatrixcells,andcellsoftheouterandinnerrootsheats.Expressedincolon. PTM:PhosphorylationbyGSK3BrequirespriorphosphorylationofSer-45byanotherkinase.PhosphorylationproceedsthenfromThr-41toSer-37andSer-33.&EGFstimulatestyrosinephosphorylation.PhosphorylationonTyr-654decreasesCDH1bindingandenhancesTBPbinding.&UbiquitinatedbyaE3ubiquitinligasecomplexcontainingUBE2D1,SIAH1,CACYBP/SIP,SKP1A,APCandTBL1X(Probable).Itsubiquitinationleadstoitssubsequentproteasomaldegradation. DISEASE:ActivatingmutationsinCTNNB1haveoncogenicactivityresultingintumordevelopment.Somaticmutationsarefoundinvarioustumortypes,includingcoloncancers,ovarianandprostatecarcinomas,hepatoblastoma(HB),hepatocellularcarcinoma(HCC).HBsaremalignantembryonaltumorsmainlyaffectingyoungchildreninthefirstthreeyearsoflife.&DefectsinCTNNB1areacauseofpilomatrixoma(PTR)[MIM:132600];acommonbenignskintumor.&DefectsinCTNNB1areacauseofmedulloblastoma(MDB)[MIM:155255].MDBisamalignant,invasiveembryonaltumorofthecerebellumwithapreferentialmanifestationinchildren.&AchromosomalrearrangementinvolvingCTNNB1maybeacauseofsalivaryglandpleiomorphicadenomas(PA)[181030].Pleiomorphicadenomasarethemostcommonbenignepithelialtumorsofthesalivarygland.Translocationt(3;8)(p21;q12)withPLAG1." SIMILARITY:Belongstothebeta-cateninfamily.&Contains12ARMrepeats. |
| MolecularWeight | ~86kDaobserved. |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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