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Millipore/04-1070 | Anti-phospho-β-Catenin(Ser33/Ser37) Antibody, clone EP742(2)Y, rabbit monoclonal/04-1070/100 µ
Millipore/04-1070 | Anti-phospho-β-Catenin(Ser33/Ser37) Antibody, clone EP742(2)Y, rabbit monoclonal/04-1070/100 µ
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 04-1070 |
| Description | Anti-phospho-β-Catenin(Ser33/Ser37)Antibody,cloneEP742(2)Y,rabbitmonoclonal |
| AlternateNames |
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| BackgroundInformation | β-CateninlinksCadherincelladhesionproteinstotheCytoskeletonandhasrecentlybeenimplicatedintheWnt/Winglesssignalingpathwayandintheprogressionofneoplasia.β-CateninassociateswiththetumorsuppressorAdenomatousPolypopsisColi(APC)andfunctionsasaco-activatoroftheTCFtranscriptionfactorfamily.ItisregulatedbyGlycogenSynthaseKinase(GSK)3b,whichphosphorylatesandtargetsβ-Cateninforubiquitin-mediatedproteolysis.StABIlizationofβ-Catenin,eitherbyWnt-mediatedinhibitionofGSK3borbymutation(asfoundinnumerouscolorectaltumorsandmelanomacelllines),resultsinactivationofTCFresponsivegeneswhicharethoughttoplaykeyrolesindevelopmentandcancerprogression.δ-Catenin(NPRAP/Neurojungin),amemberofthep120Cateninsubfamily,isastructuralproteininvolvedinneuronaldevelopment.ItinteractswithActin,CortactinandSrcKinasestopromoteneuriteelongationorbranching,dependinguponitsphosphorylationstate. |
| ProductInformation | |
|---|---|
| Format | Unpurified |
| Control |
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| Presentation | RabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20ºCfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Phospho-specificpeptidecorrespondingtoresiduessurroundingSerine33andSerine37ofhumanβ-catenin. |
| Epitope | phospho-Ser33/Ser37 |
| Clone | EP742(2)Y |
| Host | Rabbit |
| Specificity | Thisantibodydetectsβ-cateninphosphorylatedonSerine33and/orSerine37neartheN-terminus. |
| Isotype | IgG |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Beta-cateninisanadherensjunctionprotein.Adherensjunctions(AJs;alsocalledthezonulaadherens)arecriticalfortheestablishmentandmaintenanceofepitheliallayers,suchasthoseliningorgansurfaces.AJsmediateadhesionbetweencells,communicateasignalthatneighboringcellsarepresent,andanchortheactincytoskeleton.Inservingtheseroles,AJsregulatenormalcellgrowthandbehavior.Atseveralstagesofembryogenesis,woundhealing,andtumorcellmetastasis,cellsformandleaveepithelia.Thisprocess,whichinvolvesthedisruptionandreestablishmentofepithelialcell-cellcontacts,mayberegulatedbythedisassemblyandassemblyofAJs.AJsmayalsofunctioninthetransmissionofthe"contactinhibition"signal,whichinstructscellstostopdividingonceanepithelialsheetiscomplete.[suppliedbyOMIM]. |
| GeneSymbol |
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| Modifications |
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| PurificationMethod | Unpurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:InvolvedintheregulationofcelladhesionandinsignaltransductionthroughtheWntpathway. SUBUNITSTRUCTURE:Twoseparatepoolsarefoundinthecytoplasm:oneisPSEN1/cadherin/catenincomplexwhichanchorstotheactincytoskeleton.TheotherpoolispartofalargecomplexcontainingAXIN1,AXIN2,APC,CSNK1A1andGSK3BthatpromotesphosphorylationonN-terminalSerandThrresiduesandubiquitinationofCTNNB1viaBTRCanditssubsequentdegradationbytheproteasome.Wnt-dependentactivationofDVLantagonizestheactionofGSK3B.WhenGSK3Bactivityisinhibitedthecomplexdissociates,CTNNB1isdephosphorylatedandisnolongertargetedfordestruction.Thestabilizedproteintranslocatestothenucleus,whereitbindsTCF/LEF-1familymembers,TBP,BCL9andpossIBLyalsoRUVBL1andCHD8.BindsCTNNBIPandEP300.CTNNB1formsaternarycomplexwithLEF1andEP300thatisdisruptedbyCTNNBIP1bindingBysimilarity.InteractswithTAX1BP3(viathePDZdomain);thisinteractioninhibitsthetranscriptionalactivityofCTNNB1Bysimilarity.InteractswithAJAP1,BAIAP1,CARM1,CTNNA3,CXADRandPCDH11Y.BindsSLC9A3R1.InteractswithGLIS2andMUC1.InteractswithSLC30A9.InteractswithXIRP1Bysimilarity.InteractswithPTPRU(viathecytoplasmicjuxtamembranedomain). SUBCELLULARLOCATION:Cytoplasm.Nucleus.Cytoplasm›cytoskeleton.Note=Cytoplasmicwhenitisunstabilized(highlevelofphosphorylation)orboundtoCDH1.Translocatestothenucleuswhenitisstabilized(lowlevelofphosphorylation).InteractionwithGLIS2andMUC1promotesnucleartranslocation. TISSUESPECIFICITY:Expressedinseveralhairfolliclecelltypes:basalandperipheralmatrixcells,andcellsoftheouterandinnerrootsheats.Expressedincolon. PTM:PhosphorylationbyGSK3BrequirespriorphosphorylationofSer-45byanotherkinase.PhosphorylationproceedsthenfromThr-41toSer-37andSer-33. EGFstimulatestyrosinephosphorylation.PhosphorylationonTyr-654decreasesCDH1bindingandenhancesTBPbinding. UbiquitinatedbyaE3ubiquitinligasecomplexcontainingUBE2D1,SIAH1,CACYBP/SIP,SKP1A,APCandTBL1XProbable.Itsubiquitinationleadstoitssubsequentproteasomaldegradation. DISEASE:ActivatingmutationsinCTNNB1haveoncogenicactivityresultingintumordevelopment.Somaticmutationsarefoundinvarioustumortypes,includingcoloncancers,ovarianandprostatecarcinomas,hepatoblastoma(HB),hepatocellularcarcinoma(HCC).HBsaremalignantembryonaltumorsmainlyaffectingyoungchildreninthefirstthreeyearsoflife. DefectsinCTNNB1areacauseofpilomatrixoma(PTR)[MIM:132600];acommonbenignskintumor. DefectsinCTNNB1areacauseofmedulloblastoma(MDB)[MIM:155255].MDBisamalignant,invasiveembryonaltumorofthecerebellumwithapreferentialmanifestationinchildren. AchromosomalrearrangementinvolvingCTNNB1maybeacauseofsalivaryglandpleiomorphicadenomas(PA)[181030].Pleiomorphicadenomasarethemostcommonbenignepithelialtumorsofthesalivarygland.Translocationt(3;8)(p21;q12)withPLAG1. SIMILARITY:Belongstothebeta-cateninfamily. Contains12ARMrepeats. |
| MolecularWeight | ~92kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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