Millipore/S7810 | CpG WIZ®RB1-甲基化特异性PCR检测/S7810/25检测
市场价：
¥10380.00
美元价：
6228.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
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| CatalogueNumber | S7810 |
| BrandFamily | Chemicon® |
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| Description | CpGWIZ®RB1-MethylationspecificPCRassay |
| Overview | MSP,performedusingtheCpGenome™DNAModificationKitandtheCpGWIZ®RB1AmplificationKit,permitssensitivedetectionofalteredDNA.BecausethisisaPCR-basedassay,itisextremelysensitive,facilitatingthedetectionoflownumbersofmethylatedallelesandthestudyofsamplescontainingsmallamountsofDNA.MSPalsoallowsexaminationofallCpGsites,notjustthosewithinsequencesrecognizedbymethylationsensitiverestrictionenzymes.Increasingthenumberofsuchsiteswhichcanbeassessedallowsrapid,finemappingofmethylationpatternsthroughoutCpGregions.Inaddition,thebisulfitemodificationisideallysuitedforanalysisofCpGislandssinceitconvertsthemajorityofcytosinestouracils,makingaregionofthegenomewhichisCG-richlessdifficulttoamplifybyPCR. Methylation-specificPCR(MSP)employsaninitialbisulfitereactiontomodifytheDNA,followedbya"hotstart"PCRamplificationwithspecificprimersdesignedtodistinguishmethylatedDNAfromunmethylatedDNA.AsshowninFigure1,inthebisulfitereaction,allunmethylatedcytosinesareconvertedtouracilswhile5-methylcytosinesremainunaltered.Thus,thesequenceofthetreatedDNAwilldifferiftheDNAisoriginallymethylatedvs.unmethylated.PrimerscontainedintheCpGWIZ®RB1AmplificationKitaredesignedtospecificallyamplifyeachofthesequencesbaseduponthesechemically-induceddifferences.IfthesampleDNAwasoriginallyunmethylated,aproductwillbegeneratedafterPCRusingtheUprimerset.Conversely,aproductwillbegeneratedusingtheMprimersetifthesamplewasoriginallymethylated. |
| BackgroundInformation | Methylationofcytosineslocated5"toguanosineisknowntohaveaprofoundeffectontheexpressionofseveraleukaryoticgenes(1).Innormalcells,methylationoccurspredominantlyinCG-poorregions,whileCG-richareas,calledCpGislands,remainunmethylated.AberrantmethylationofnormallyunmethylatedCpGislandshasbeendocumentedasarelativelyfrequenteventinimmortalizedandtransformedcells(2)andhasbeenassociatedwithtranscriptionalinactivationofdefinedtumorsuppressorgenesinhumancancers(3,4).Theretinoblastoma1(RB1)geneexhibitscharacteristichypermethylationimmanymaligninanttumors. PreviouslydevelopedmethodstodeterminethemethylationstatusofcytosineincludedigestionwithmethylationsensitiverestrictionenzymesandgenomicDNAsequencing.Bothtechniqueshavelimitations:restrictionenzymescanonlydetectmethylationsiteswithintheirrecognitionsequenceandsequencingistimeconsuming.IncreasingthedetectionsensitivityofCpGislandmethylationhasthepotentialtodefinetumorsuppressorgenefunctionandprovidesanewstrategyforearlytumordetection. Methylation-specificPCR(MSP)isanewtechnologyforsensitivedetectionofabnormalgenemethylationutilizingsmallamountsofDNA(5).ThisprocessemploysaninitialbisulfitereactiontomodifytheDNA,followedbyPCRamplificationwithspecificprimersdesignedtodistinguishmethylatedfromunmethylatedDNA.TheCpGenome™DNAModificationKit(S7820)containsthereagentsnecessarytoperformtheinitialbisulfitereactions,whiletheCpGWIZ®RB1AmplificationKitcontainsthereagentsrequiredforthePCRamplificationreactions. |
| MaterialsRequiredbutNotDelivered | EquipmentandSupplies a.MicrocentrifugetubesforPCRamplification b.Aerosol-resistantPipettetips c.Thermocycler d.Gelelectrophoresisapparatus(verticalorhorizontal) e.PowerSupply f.302nmUVtransIlluminator,cameraandfilm Reagents a2.5mMdNTPmix(2.5mMofeachnucleotide) b."Hotstart"Taqpolymerase c."Hotstart"PCRreagents(seeSec.II.Protocols). d.Reagentsforgelelectrophoresis(1XTBEand2%agarose,10%acrylamide,orsuitablehighresolutionagarose) e.DNAMarkers(sizerange100-300bp) f.Ethidiumbromide(10mg/mL) g.Gel-loADIngsolution/LoadingDye h.BisulfiteModifiedDNA(CpGenome™DNAModificationKit,S7820) |
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| Applications | |
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| Application | MSP,performedusingtheCpGenomeDNAModificationKit&theCpGWIZRB1AmplificationKit,permitssensitivedetectionofalteredDNA. |
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| BIOLOGicalInformation | |
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| EntrezGeneNumber | |
| EntrezGeneSummary | Retinoblastoma(RB)isanembryonicmalignantneoplasmofretinalorigin.Italmostalwayspresentsinearlychildhoodandisoftenbilateral.Spontaneousregression("cure")occursinsomecases.TheretinoblastomageneRBwasthefirsttumorsuppressorgenecloned,andisanegativeregulatorofthecellcyclethroughitsABIlitytobindthetranscriptionfactorE2F(MIM189971)andrepresstranscriptionofgenesrequiredforSphase(HanahanandWeinberg,2000[PubMed10647931]).[suppliedbyOMIM] |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P06400#Keyregulatorofentryintocelldivisionthatactsasatumorsuppressor.Directlyinvolvedinheterochromatinformationbymaintainingoverallchromatinstructureand,inparticular,thatofconstitutiveheterochromatinbystabilizinghistonemethylation.RecruitsandtargetshistonemethyltransferasesSUV39H1,SUV420H1andSUV420H2,leadingtoepigenetictranscriptionalrepression.ControlshistoneH4"Lys-20"trimethylation.AlsoactsasatranscriptionrepressorofE2Ftargetgenesbyrecruitingchromatin-modifyingenzymestopromoters.InhibitstheintrinsickinaseactivityofTAF1.FormsacomplexwithadenovirusE1AandwithSV40largeTantigen.MaybindandmodulatefunctionallycertaincellularproteinswithwhichTandE1Acompeteforpocketbinding. SIZE:928aminoacids;106159Da SUBUNIT:InteractspreferentiallywithtranscriptionfactorE2F1.TheunphosphorylatedforminteractswithARID3B,JARID1A,SUV39H1,MJD2A/JHDM3AandTHOC1.InteractswiththeN-terminaldomainofTAF1.InteractswithAATF,DNMT1,LIN9,LMNA,SUV420H1,SUV420H2,PELP1andTMPO-alpha.MayinteractwithKNTC2.InteractswithEID1andZUBR1.InteractswithARID4AandJARID1B. SUBCELLULARLOCATION:Nucleus. TISSUESPECIFICITY:Expressedintheretina. PTM:PhosphorylatedfromStoMphaseofthecellcycleandisdephosphorylatedinG1.T,butnotE1A,bindsonlytotheunphosphorylatedform. DISEASE:SwissProt:P06400#DefectsinRB1arethecauseofchildhoodcancerretinoblastoma(RB)[MIM:180200].RBisacongenitalmalignanttumorthatarisesfromthenuclearlayersoftheretina.Itoccursinabout1:20"000livebirthsandrepresentsabout2%ofchildhoodmalignancies.Itisbilateralinabout30%ofcases.AlthoughmostRBappearsporadically,about20%aretransmittedasanautosomaldominanttraitwithincompletepenetrance.Thediagnosisisusuallymadebeforetheageof2yearswhenstrabismusoragraytoyellowreflexfrompupil(cateye)isinvestigated.&DefectsinRB1areacauseofbladdercancer[MIM:109800].&DefectsinRB1areacauseofosteogenicsarcoma[MIM:259500]. SIMILARITY:SwissProt:P06400##Belongstotheretinoblastomaprotein(RB)family. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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