微孔/ABC132 |抗磷酸Rb（Ser807/Ser811）抗体/ABC132/100µ；G
市场价：
¥6580.00
美元价：
3948.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ABC132 |
| Description | Anti-phospho-Rb(Ser807/Ser811)Antibody |
| AlternateNames |
|
| BackgroundInformation | Theretinoblastoma(Rb)geneproduct(alsoknownasp105,p110orpRb)isatumorsuppressorproteinthatismissingormutatedinavarietyofhumantumors.Inthehereditarychildhoodformofretinoblastoma,Rbislostfromchromosome13.RbregulatescellproliferationanddifferentiationbycontrollingprogressionthroughtherestrictionpointwithintheG1phaseofthecellcycle.Rbformscomplexeswithmanyproteins,andthisbindingactivityisrequiredforgrowthsuppression.RbspecificallyinteractswiththeE2FfamilyoftranscriptionfactorstorepressE2F-mediatedtranscription.RbalsointeractsandalterstheactivityofothertranscriptionfactorsincludingElf-1,ATF-2,MyoD,BRG-1,c-myc,N-myc.Rbrecruitsahistonedeacetylase,HDAC1,toE2F-1torepresstranscription.TwoRbrelatedproteinsp107andp130alsofunctiontoregulatespecificmembersoftheE2Ftranscriptionfactorfamily. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Control |
|
| Presentation | Purifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugatedlinearpeptidecorrespondingtohumanRbphosphorylatedatSer807/Ser811. |
| Epitope | PhosphorylatedSer807/Ser811 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | ThisantibodyrecognizesRbphosphorylatedatSer807/Ser811. |
| SpeciesReactivity |
|
| SpeciesReactivityNote | DemonstratedtoreactwithHuman. PredictedtoreactwithMousebasedon100%sequencehomology.(11/12aminoacidsidenticalinRatandchickenRb). |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Theproteinencodedbythisgeneisanegativeregulatorofthecellcycleandwasthefirsttumorsuppressorgenefound.TheencodedproteinalsostABIlizesconstitutiveheterochromatintomaintaintheoverallchromatinstructure.Theactive,hypophosphorylatedformoftheproteinbindstranscriptionfactorE2F1.Defectsinthisgeneareacauseofchildhoodcancerretinoblastoma(RB),bladdercancer,andosteogenicsarcoma.[providedbyRefSeq]. |
| GeneSymbol |
|
| Modifications |
|
| PurificationMethod | AffinityPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:Keyregulatorofentryintocelldivisionthatactsasatumorsuppressor.ActsasatranscriptionrepressorofE2F1targetgenes.Theunderphosphorylated,activeformofRB1interactswithE2F1andrepressesitstranscriptionactivity,leADIngtocellcyclearrest.Directlyinvolvedinheterochromatinformationbymaintainingoverallchromatinstructureand,inparticular,thatofconstitutiveheterochromatinbystabilizinghistonemethylation.RecruitsandtargetshistonemethyltransferasesSUV39H1,SUV420H1andSUV420H2,leadingtoepigenetictranscriptionalrepression.ControlshistoneH4"Lys-20"trimethylation.InhibitstheintrinsickinaseactivityofTAF1.MediatestranscriptionalrepressionbySMARCA4/BRG1byrecruitingahistonedeacetylase(HDAC)complextothec-FOSpromoter.Inrestingneurons,transcriptionofthec-FOSpromoterisinhibitedbyBRG1-dependentrecruitmentofaphospho-RB1-HDAC1repressorcomplex.Uponcalciuminflux,RB1isdephosphorylatedbycalcineurin,whichleadstoreleaseoftherepressorcomplex(Bysimilarity).Incaseofviralinfections,interactionswithSV40largeTantigen,HPVE7proteinoradenovirusE1AproteininducethedisassemblyofRB1-E2F1complextherebydisruptingRB1"sactivity. SUBUNITSTRUCTURE:InteractswithATAD5.InteractswithPRMT2(Bysimilarity).ThehypophosphorylatedforminteractswithandsequesterstheE2F1transcriptionfactor.InteractswithheterodimericE2F/DPtranscriptionfactorcomplexescontainingTFDP1andeitherE2F1,E2F3,E2F4orE2F5,orTFDP2andE2F4.TheunphosphorylatedforminteractswithEID1,ARID3B,KDM5A,SUV39H1,MJD2A/JHDM3AandTHOC1.InteractswiththeN-terminaldomainofTAF1.InteractswithAATF,DNMT1,LIN9,LMNA,SUV420H1,SUV420H2,PELP1andTMPO-alpha.MayinteractwithNDC80.InteractswithGRIP1andUBR4.InteractswithARID4AandKDM5B.InteractswithE4F1andLIMD1.InteractswithSMARCA4/BRG1ANDHDAC1(Bysimilarity).InteractswithadenovirusE1Aprotein,HPVE7proteinandSV40largeTantigen.InteractswithPSMA3andUSP4.Interacts(whenmethylatedatLys-860)withL3MBTL1. SUBCELLULARLOCATION:Nucleus. TISSUESPECIFICITY:Expressedintheretina. DOMAIN:ThePocketdomainbindstothethreonine-phosphorylateddomainC,therebypreventinginteractionwithheterodimericE2F/DPtranscriptionfactorcomplexes. PTM:PhosphorylatedinG1,therebyreleasingE2F1whichisthenabletoactivatecellgrowth.DephosphorylatedatthelateMphase.SV40largeTantigen,HPVE7andadenovirusE1Abindtotheunderphosphorylated,activeformofpRb.PhosphorylationatThr-821andThr-826promotesinteractionbetweentheC-terminaldomainCandthePocketdomain,andtherebyinhibitsinteractionswithheterodimericE2F/DPtranscriptionfactorcomplexes.DephosphorylatedatSer-795bycalcineruinuponcalciumstimulation. N-terminusismethylatedbyMETTL11A/NTM1(Bysimilarity).MonomethylatedatLys-860bySMYD2,promotinginteractionwithL3MBTL1. INVOLVEMENTINDISEASE:DefectsinRB1arethecauseofchildhoodcancerretinoblastoma(RB)[MIM:180200].RBisacongenitalmalignanttumorthatarisesfromthenuclearlayersoftheretina.Itoccursinabout1:20"000livebirthsandrepresentsabout2%ofchildhoodmalignancies.Itisbilateralinabout30%ofcases.AlthoughmostRBappearsporadically,about20%aretransmittedasanautosomaldominanttraitwithincompletepenetrance.Thediagnosisisusuallymadebeforetheageof2yearswhenstrabismusoragraytoyellowreflexfrompupil("cateye")isinvestigated. DefectsinRB1areacauseofsusceptibilitytobladdercancer(BLC)[MIM:109800].Amalignancyoriginatingintissuesoftheurinarybladder.Itoftenpresentswithmultipletumorsappearingatdifferenttimesandatdifferentsitesinthebladder.Mostbladdercancersaretransitionalcellcarcinomas.Theybeginincellsthatnormallymakeuptheinnerliningofthebladder.Othertypesofbladdercancerincludesquamouscellcarcinoma(cancerthatbeginsinthin,flatcells)andadenocarcinoma(cancerthatbeginsincellsthatmakeandreleasemucusandotherfluids).Bladdercancerisacomplexdisorderwithbothgeneticandenvironmentalinfluences. DefectsinRB1areacauseofosteogenicsarcoma(OSRC)[MIM:259500]. SEQUENCESIMILARITIES:Belongstotheretinoblastomaprotein(RB)family. |
| MolecularWeight | ~110kDaobserved |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
