密理博/07-1401 |抗GLUT-1抗体，CT/07-1401/50µ；G
市场价：
¥5740.00
美元价：
3444.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 07-1401 |
| Replaces | AB1340 |
| Description | Anti-GLUT-1Antibody,CT |
| AlternateNames |
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| BackgroundInformation | GLUTs(Glucosetransporters)areafamilyofintegralmembraneproteinsthatfacilitativeglucoseuptakebycells.Theyareintegralmembraneglycoproteinsinvolvedintransportingglucoseintomostcells.Seventypesofglucosetransportcarrierproteins,designatedasGLUT1to7,facilitateglucosetransportacrossthecellmembrane.Molecularcloningofglucosetransportershaveidentifiedafamilyofcloselyrelatedgenesthatencodeatleast7proteinsexhibitinghighdegreeofaminoacidhomology(45%to65%),allinthemolecularweightrangeof40to60kDa.GLUT-1functionsinconstitutiveorbasalglucoseuptakeinadulttissuesandisthepredominantglucosetransporterinembryonicandfetaltissues.Ithastwoforms,a55kDaanda45kDa.The55kDaformofGLUT1regulatesimportofglucosefrombloodtobrainacrosstheendothelialcellsoftheblood-brainbarrier(BBB),whereasthe45kDaformofGLUT1predominantlyregulatesnonvascularglialglucoseuptake.GLUT2isafacilitativeglucosetransporter.ThisisoformlikelymediatesthebidirectionaltransferofglucoseacrosstheplasmamembraneofhepatocytesandisresponsIBLeforuptakeofglucosebythebetacells;maycomprisepartoftheglucose-sensingmechanismofthebetacell.MayalsoparticipatewiththeNa(+)/glucosecotransporterinthetranscellulartransportofglucoseinthesmallintestineandkidney.GLUT-4ispresentinskeletalmuscle,heartandADIposetissues.GLUT-4ishighlyconservedbetweenspecies.TheexpressionofGLUT-4isgreatlyinfluencedbyinsulinandotherstimulatorsoftheIP3signalingpathway.BindingofinsulintotheinsulinreceptorsignalsthroughPI3KandAkttotranslocateGLUT-4totheplasmamembranewhereitallowstheuptakeofglucose. |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Control |
|
| Presentation | PurifiedrabbitpolyclonalIgGinbuffercontaining0.02MPhosphateBuffer,0.25MNaCl,with0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
| Applications | |
|---|---|
| Application | Anti-GLUT-1Antibody,C-terminusisanantibodyagainstCterminusofGLUT-1thatisvalidatedforthedetectionofGLUT-1inWesternBlotting(WB),Immunohistochemistry-Paraffin(IHC-P),Immunocytochemistry(ICC)andELISA. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemisty(paraffin):GLUT-1representativestainingpattern/morphologyoncolorectalcarcinoma.TissuepretreatedwithcitratepH6,antigenretrieval.Apreviouslotofthisantibodywasdilutedto1:100,usingIHC-Select®DetectionwithHRP-DAB.GLUT-1immunoreactivityinerythrocytes. Note:canbeuseasinternalcontrolforIHCrun. Immunocytochemistry:Recommended ELISA:Recommended WesternBlot:Apreviouslotofthisantibodywastestedonmultiplelysatesusingwesternblotanalysis.Seedatasheet. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | SyntheticpeptidecorrespondingaminoacidswiththeC-terminusofhumanGLUT-1coupledtoKLH(C-ELFHPLGADSQV). |
| Epitope | C-Terminus |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | ThisrabbitpolyclonalantibodydetectsGLUT-1inhumanandmousecells.Ittargetsanepitopewithin12aminoacidsfromtheC-terminalhalf. |
| Isotype | IgG |
| SpeciesReactivity |
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| SpeciesReactivityNote | Human.Mouse.PredictedtoreactwithRatbasedon100%sequencehomology. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesamajorglucosetransporterinthemammalianblood-brainbarrier.Mutationsinthisgenehavebeenfoundinafamilywithparoxysmalexertion-induceddyskinesia.[providedbyRefSeq]. |
| GeneSymbol |
|
| PurificationMethod | AffinityPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:Facilitativeglucosetransporter.Thisisoformmayberesponsibleforconstitutiveorbasalglucoseuptake.Hasaverybroadsubstratespecificity;cantransportawiderangeofaldosesincludingbothpentosesandhexoses. SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein(Bysimilarity).Melanosome.Note=Localizesprimarilyatthecellsurface(Bysimilarity).IdentifiedbymassspectrometryinmelanosomefractionsfromstageItostageIV. TISSUESPECIFICITY:Expressedatvariablelevelsinmanyhumantissues. PTM:PhosphorylateduponDNAdamage,probablybyATMorATR. DISEASE:DefectsinSLC2A1arethecauseofautosomaldominantGLUT1deficiencysyndrome[MIM:606777].Thisdiseasecausesadefectinglucosetransportacrosstheblood-brainbarrier.Itischaracterizedbyinfantileseizures,delayeddevelopment,andacquiredmicrocephaly. SIMILARITY:Belongstothemajorfacilitatorsuperfamily.Sugartransporter(TC2.A.1.1)family.Glucosetransportersubfamily[viewclassification]. |
| MolecularWeight | Thisantibodydetectsamajorband~54kDainWesternblotanalysis. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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