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Millipore/17-497 | Ras Activation ELISA ASSAY Kit/17-497/96 assays
Millipore/17-497 | Ras Activation ELISA ASSAY Kit/17-497/96 assays
市场价：
¥15700.00
美元价：
9420.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-497 |
| Replaces | 17-424 |
| BrandFamily | Upstate |
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| Description | RasActivationELISAASSAYKit |
| BackgroundInformation | RasproteinsfunctionasGDP/GTP-regulatedbinaryswitchesinsignaltransductioncascadesthatcanleadtocellgrowth,proliferation,differentiation,orsurvival.ThisRassuperfamilyhasatleastfivemajorbranchesthatincludeRas,Rho,Ran,Arf/Sar,Rab.Rasitselfhas3primaryisoforms(H-Ras,N-Ras,andK-Ras)thatdifferintheirapproximately20C-terminalaminoacids.TheyarecollectivelyreferredtoasRas.Initsactiveform,RasisboundtoGTP.Thiscausesaconformationalchangethatallowittointeractandbindtoseveraleffectormolecules,mostnotablythemembersoftheRaffamily,theRalGDSfamily,andPhosphoinositide3-kinases(PI3Kinase).RasthencleavesGTPtoGDPresultinginitsinactivation.Initsoncogenic,mutatedstate,RasisunabletohydrolyzeGTPtoGDP,thusstayinginanactivestateandactivatingnumerouspathways. Duetoitsroleasakeyregulatorofcellularfunctionsanditsimplicationsinvariouscancers,Rashasbeenapopulartargetforcancerresearchandanti-cancertherapeuticsforthepasttwodecadesforbothacademicandpharmaceuticalresearch.Itwasalsothefirsthumanoncogeneidentified.MutationsincellularRashavebeenfoundtobepresentinalargepercentageofallhumancancers.Morespecifically,K-Rasmutationsoccurfrequentlyinlung,pancreatic,andcoloncancers,whereasH-Rasmutationsareprevalentinbladder,kidney,andthyroidcancers,andN-Rasmutationsareassociatedwithmelanoma,hepatocellularcarcinoma,andleukemia(3).Inrecentstudies,RasactivityhasalsobeenimplicatedinanumberofhumandevelopmentdefectssuchasCostellosyndromeandNoonansyndrome(4). OnepaththatthepharmaceuticalindustryhastakentocontrolRasanditsactivityisbyattackingwhatsomeconsideritsAchilles’heel.Foritsactivation,Rasmustlocalizetotheplasmamembrane,butinterestingly,itlacksatransmembranedomain.Toachievethis,Rasmustfirstundergoapost-translationalmodificationknownasprenylationorgeranylationatitsC-terminalCAAXmotif.ThesedrugshaveyettopassclinicaltrialsthoughandthereisdoubtthattheywilleverbesuccessfulintreatingtumorsassociatedwithRasactivation. |
| MaterialsRequiredbutNotDelivered | 1.Multi-channelorrepeatingPipettes 2.Plateshakeratroomtemperatureandat4oC. 3.Pipettors&tipscapableofaccuratelymeasuring1-1000μL 4.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 5.Multi-channelpipettorreservoirs 6.Proteaseinhibitors 7.Nucleasefreewater/deionizedwater 8.PBS 9.LuminometerorCCDcameracoupledimagingsystem |
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| EntrezGeneSummary | K-Ras Thisgene,aKirstenrasoncogenehomologfromthemammalianrasgenefamily,encodesaproteinthatisamemberofthesmallGTPasesuperfamily.AsingleaminoacidsubstitutionisresponsIBLeforanactivatingmutation.Thetransformingproteinthatresultsisimplicatedinvariousmalignancies,includinglungadenocarcinoma,mucinousadenoma,ductalcarcinomaofthepancreasandcolorectalcarcinoma.AlternativesplicingleadstovariantsencodingtwoisoformsthatdifferintheC-terminalregion.[providedbyRefSeq] H-Ras ThisgenebelongstotheRasoncogenefamily,whosemembersarerelatedtothetransforminggenesofmammaliansarcomaretroviruses.Theproductsencodedbythesegenesfunctioninsignaltransductionpathways.TheseproteinscanbindGTPandGDP,andtheyhaveintrinsicGTPaseactivity.Thisproteinundergoesacontinuouscycleofde-andre-palmitoylation,whichregulatesitsrapidexchangebetweentheplasmamembraneandtheGolgiapparatus.MutationsinthisgenecauseCostellosyndrome,adiseasecharacterizedbyincreasedgrowthattheprenatalstage,growthdeficiencyatthepostnatalstage,predispositiontotumorformation,mentalretardation,skinandmusculoskeletalabnormalities,distinctivefacialappearanceandcardiovascularabnormalities.Defectsinthisgeneareimplicatedinavarietyofcancers,includingbladdercancer,follicularthyroidcancer,andoralsquamouscellcarcinoma.Multipletranscriptvariants,whichencodedifferentisoforms,havebeenidentifiedforthisgene.[providedbyRefSeq] |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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