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Millipore/04-775 | Anti-Ha-Ras Antibody, clone MC57, rabbit monoclonal/04-775/100 µL
Millipore/04-775 | Anti-Ha-Ras Antibody, clone MC57, rabbit monoclonal/04-775/100 µL
市场价：
¥5740.00
美元价：
3444.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 04-775 |
| Replaces | 05-775 |
| Description | Anti-Ha-RasAntibody,cloneMC57,rabbitmonoclonal |
| AlternateNames |
|
| BackgroundInformation | RasproteinsaresmallGTP-bindingproteinswhich,unliketheheterotrimericG-proteins,containallGTPaseandeffectorfunctionswithinasinglepolypeptide.AtleastthreeisoformsofRasexist,Ki-Ras,Ha-RasandN-Ras,withdistinctexpressionpatternsbutsimilarsignalingactivity.Rasispalmitoylatedandfarnesylatedatthecarboxyterminus,anchoringitinthemembrane.Inrestingcells,RasisloadedwithGDP,andisactivatedsubsequenttogrowthfactorstimulationofreceptors,whichrecruitRasGuaninenucleotideExchangeFactorstotheplaneofthemembrane.ProximityofexchangefactorstotheRasproteinscausesreleaseofGDP,anditsreplacementbyGTP.InitsGTP-boundform,Rasbindsseveralproteins,includingRaf,RalGDSandPI3Kinase.InactivationofRasoccursbyGTPhydrolysis,whichisgreatlyacceleratedbyRasGAPorNF-1,twoknownRasGTPaseActivatingProteins.ItispossIBLetoassayforRasactivationbyincubationoflysateswiththeRas-bindingdomainofRaf-1,whichselectivelybindstoRas:GTP. |
| ProductInformation | |
|---|---|
| Format | CultureSupernatant |
| Control |
|
| Presentation | Culturedsupernantantcontaining0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20ºCfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| Applications | |
|---|---|
| Application | DetectHa-RasusingthisAnti-Ha-RasAntibody,cloneMC57validatedforuseinWB. |
| KeyApplications |
|
| BIOLOGicalInformation | |
|---|---|
| Immunogen | GSTfusionproteincorrespondingtofulllengthhumanHa-Ras.CloneMC57. |
| Clone | MC57 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | RecognizesHa-Ras,Mr~21kDa.DoesnotcrossreactwithCdc42,RhoorRac1. |
| Isotype | IgG |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human.Predictedtocross-reactwithmouseandratbasedonsequencehomology. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgenebelongstotheRasoncogenefamily,whosemembersarerelatedtothetransforminggenesofmammaliansarcomaretroviruses.Theproductsencodedbythesegenesfunctioninsignaltransductionpathways.TheseproteinscanbindGTPandGDP,andtheyhaveintrinsicGTPaseactivity.Thisproteinundergoesacontinuouscycleofde-andre-palmitoylation,whichregulatesitsrapidexchangebetweentheplasmamembraneandtheGolgiapparatus.MutationsinthisgenecauseCostellosyndrome,adisease characterizedbyincreasedgrowthattheprenatalstage,growthdeficiencyatthepostnatalstage,predispositiontotumorformation,mentalretardation,skinandmusculoskeletalabnormalities,distinctivefacialappearanceandcardiovascularabnormalities.Defectsinthisgeneareimplicatedinavarietyofcancers,includingbladdercancer,follicularthyroidcancer,andoralsquamouscellcarcinoma.Multipletranscriptvariants,whichencodedifferentisoforms,havebeenidentifiedforthisgene. |
| GeneSymbol |
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:RasproteinsbindGDP/GTPandpossessintrinsicGTPaseactivity.Ref.18Ref.32 ENZYMEREGULATION:AlternatebetweenaninactiveformboundtoGDPandanactiveformboundtoGTP.Activatedbyaguaninenucleotide-exchangefactor(GEF)andinactivatedbyaGTPase-activatingprotein(GAP). SUBUNITSTRUCTURE:InitsGTP-boundforminteractswithPLCE1.InteractswithTBC1D10C.InteractswithRGL3Bysimilarity.FormsasignalingcomplexwithRASGRP1andDGKZ.InteractswithRASSF5. SUBCELLULARLOCATION:Cellmembrane;Lipid-anchor;Cytoplasmicside.Golgiapparatusmembrane;Lipid-anchor.Note:ShuttlesbetweentheplasmamembraneandtheGolgiapparatus. PTM:PalmitoylatedbytheZDHHC9-GOLGA7complex.Acontinuouscycleofde-andre-palmitoylationregulatesrapidexchangebetweenplasmamembraneandGolgi.S-nitrosylated;criticalforredoxregulation.Importantforstimulatingguaninenucleotideexchange.Nostructuralperturbationonnitrosylation. INVOLVEMENTINDISEASE:DefectsinHRASarethecauseofCostellosyndrome[MIM:218040];alsoknownasfaciocutaneoskeletalsyndrome.Costellosyndromeisarareconditioncharacterizedbyprenatallyincreasedgrowth,postnatalgrowthdeficiency,mentalretardation,distinctivefacialappearance,cardiovascularabnormalities(typicallypulmonicstenosis,hypertrophiccardiomyopathyand/oratrialtachycardia),tumorpredisposition,skinandmusculoskeletalabnormalities. DefectsinHRASarethecauseofcongenitalmyopathywithexcessofmusclespindles(CMEMS)[MIM:218040].CMEMSisavariantofCostellosyndrome. DefectsinHRASmaybeacauseofsusceptibilitytoHurthlecellthyroidcarcinoma[MIM:607464];alsoknownasHurthlecellthyroidneoplasia.Hurthlecellthyroidcarcinomaaccountsforapproximately3%ofallthyroidcancers.Althoughtheyareclassifiedasvariantsoffollicularneoplasms,theyaremoreoftenmultifocalandsomewhatmoreaggressiveandarelesslikelytotakeupiodinethanareotherfollicularneoplasms.Mutationswhichchangepositions12,13or61activatethepotentialofHRAStotransformculturedcellsandareimplicatedinavarietyofhumantumors.DefectsinHRASareacauseofbladdercancer.DefectsinHRASarethecauseoforalsquamouscellcarcinoma(OSCC). SEQUENCESIMILARITIES:BelongstothesmallGTPasesuperfamily.Rasfamily. MASSSPECTROMETRY:Molecularmassis6.223±2Dafrompositions112-166.DeterminedbyESI.Molecularmassis6.253±2Dafrompositions112-166.DeterminedbyESI.Includesonenitricoxidemolecule. |
| MolecularWeight | ~21kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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