4000-520-616
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当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/17-613 | ChIPAb+p53-芯片验证抗体和引物集/17-613/25分析每个试剂盒25次分析~每小时1μg
商品详细Millipore/17-613 | ChIPAb+p53-芯片验证抗体和引物集/17-613/25分析每个试剂盒25次分析~每小时1μg
Millipore/17-613 | ChIPAb+p53-芯片验证抗体和引物集/17-613/25分析每个试剂盒25次分析~每小时1μg
Millipore/17-613 | ChIPAb+p53-芯片验证抗体和引物集/17-613/25分析每个试剂盒25次分析~每小时1μg
商品编号: 17-613
品牌: 密理博
市场价: ¥8000.00
美元价: 4800.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumber17-613
BrandFamilyUpstate
TradeName
  • ChIPAb+
  • Upstate
DescriptionChIPAb+p53-ChIPValidatedAntibodyandPrimerSet
OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
TheChIPAb+p53setincludesthep53antibodyraisedagainstrecombinanthumanp53,thenegativecontrolantibody(mousenormalIgG),andqPCRprimersflankinganSp1bindingsite(towhichp53canberecruited1)withinthehumanp21(WAF1/CIP1/CDKN1A)promoter,amplifyinga105basepairPCRproduct.Thep53andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofp53associatedchromatin.
ProductInformation
FormatPurified
Control
  • IncludednegativecontrolantibodymousenormalIgGandcontrolprimersspecificforhumanp21(WAF1/CIP1/CDKN1A)promoter.
PresentationAnti-p53(mousemonoclonalIgG).Onevialcontaining25μgofofproteinApurifiedmouseIgG2ain25μLofstoragebuffer(PBS,pH7.4,containing0.05%sodiumazideand1mg/mLBSA).Storeat-20°C.

NormalMouseIgG.Onevialcontaining25μgofnormalmouseIgGin25μLvolume.Storeat-20°C.

ChIPPrimersp21.Onevialcontaining75μLof5μMofeachprimerspecificforaregionofthehumanp21(WAF1/CIP1/CDKN1A)promoter.Storeat-20°C.
FOR:GTGGCTCTGATTGGCTTTCTG
REV:CTGAAAACAGGCAGCCCAAG
StorageandShippingInformation
StorageConditionsStablefor1yearat-20°Cfromdateofreceipt
Applications
ApplicationThisChIPAb+p53-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
KeyApplications
  • WesternBlotting
  • ChromatinImmunoprecipitation(ChIP)
ApplicationNotesChromatinImmunoprecipitationAnalysis:
SonicatedchromatinpreparedfromuntreatedoractinomycinDtreated(30ng/mL,6hrs.)U2OScells(3X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing1μgofmouseAnti-p53andtheMagnaChIP™G(Cat.#17-611)Kit.Immunoprecipitationofp53associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimersp21flankingthehumanp21promoterthatcontainsanSp1bindingsite(Pleaseseefigures).
FoldIncreaseisaratioofnormalizedmeanIPquantitiesextractedfromstandardcurvesderivedfrominputsofeachchromatinsample.Aspreviouslypublished,p53associationwiththisregionofthep21promoterincreasesuponactinomycinDtreatment.

ChromatinImmunoprecipitationAnalysis:
SonicatedchromatinpreparedfromuntreatedorUVtreated(6hrs,50joules/m2.)U2OScells(3X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing1μgofmouseAnti-p53andtheMagnaChIP™A(Cat.#17-610)Kit.
Immunoprecipitationofp53associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimersp21flankingthehumanp21promoterthatcontainsanSp1bindingsite(Pleaseseefigures).
FoldIncreaseisaratioofnormalizedmeanIPquantitiesextractedfromstandardcurvesderivedfrominputsofeachchromatinsample.p53immunoprecipitableactivityassociatedwiththispromoterincreaseswithUVtreatmentasobservedinotherstudies.
BiologicalInformation
HostMouse
SpecificityRecognizesp53inwildtypeandmutantformsofhumanorigin.
IsotypeIgG
SpeciesReactivity
  • Human
AntibodyTypeMonoclonalAntibody
EntrezGeneNumber
GeneSymbol
  • P53
  • TP53
PurificationMethodProteinAPurfied
UniProtNumber
MolecularWeight53kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。