
微孔/OP29 |抗p53(Ab-3)(突变型)小鼠单克隆抗体(PAb240)/OP29/
市场价:
¥0.00
美元价:
0.00
产品分类:
功能性抗体
公司分类:
Functional_antibody
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description | |
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Overview | Recognizesthe~53kDamutantp53proteinundernon-denaturingconditionsbyimmunoprecipitation,immunofluorescence,andflowcytometry.Recognizesbothmutantandwild-typep53byimmunoblottingandparaffinsectionsunderdenaturingconditions. |
CatalogueNumber | OP29 |
BrandFamily | Calbiochem® |
ProductInformation | |
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Form | Liquid |
Formulation | In50mMsodiumphosphatebuffer,0.2%gelatin,pH7.5. |
Negativecontrol | SK-OV-3cells |
Positivecontrol | A431,Hs27(wild-typep53),orSK-BR-3cellsorbreastcarcinomatissue |
Preservative | ≤0.1%sodiumazide |
StorageandShippingInformation | |
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ShipCode | BlueIceOnly |
Toxicity | StandardHandling |
Storage | +2°Cto+8°C |
Donotfreeze | Yes |
Applications | |
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ApplicationReferences | ParaffinSectionsPezzella,F.,etal.1994.J.Clin.Pathol.47,592.OriginalCloneGannon,J.V.,etal.1990.EMBOJ.9,1595.EpitopeIdentificationStephen,C.W.andLane,D.P.,1992.J.Mol.Biol.225,1.ImmunofluorescenceBernardi,R.,etal.2004.Nat.CellBiol.6,665. |
KeyApplications | Immunoblotting(WesternBlotting) Immunofluorescence Immunoprecipitation ParaffinSections FlowCytometry FrozenSections GelShift |
ApplicationNotes | FlowCytometry(1-20µg/ml) FrozenSections(10µg/ml) GelShift(seecomments) Immunoblotting(5µg/ml) Immunofluorescence(1-20µg/ml,seeapplicationreferences) Immunoprecipitation(1µgpersample) ParaffinSections(seeapplicationreferences) |
ApplicationComments | Undernon-denaturingconditions(immunoprecipitation,immunofluorescenceandfrozensections),Anti-p53(Ab-3)doesnotrecognizenormal(wild-type)p53protein;itrecognizesanepitopeexposedbyactivatingmutationsordenaturation.Indenaturingprotocols(immunoblottingandparaffinsections),Anti-p53(Ab-3)willrecognizebothmutantandwild-typep53.Willnotrecognizetosomep53moleculeswithmutationsintheRHSVVepitope,butwillreacttoTFIIIA,whichhastheRHSVVepitope.Forgelshiftassay,useCat.No.OP29LandresUSPendin100µlbuffer.Antibodyshouldbetitratedforoptimalresultsinindividualsystems. |
BIOLOGicalInformation | |
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Immunogen | arecombinantproteinconsistingofaminoacids14-389ofp53fusedtoβ-galactosidase |
Immunogen | Human |
Epitope | withinaminoacids213-217 |
Clone | PAb240 |
Host | Mouse |
Isotype | IgG₁ |
SpeciesReactivity |
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AntibodyType | MonoclonalAntibody |
ConcentrationLabel | Pleaserefertoviallabelforlot-specificconcentration |
PhysicochemicalInformation |
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Dimensions |
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MaterialsInformation |
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MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。
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