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当前位置: 首页 > 产品中心 > Functional_antibody > 密理博/APT420 |卡斯帕塔格™ 泛半胱天冬酶原位分析试剂盒,荧光素/APT420/25分析
商品详细密理博/APT420 |卡斯帕塔格™ 泛半胱天冬酶原位分析试剂盒,荧光素/APT420/25分析
密理博/APT420 |卡斯帕塔格™ 泛半胱天冬酶原位分析试剂盒,荧光素/APT420/25分析
密理博/APT420 |卡斯帕塔格™ 泛半胱天冬酶原位分析试剂盒,荧光素/APT420/25分析
商品编号: APT420
品牌: 密理博
市场价: ¥5080.00
美元价: 3048.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberAPT420
BrandFamilyChemicon®
TradeName
  • CaspaTag
  • Chemicon
DescriptionCaspaTag™Pan-CaspaseInSituAssayKit,Fluorescein
BackgroundInformationApoptosisisanevolutionarilyconservedformofcellsuicide,whichfollowsaspecializedcellularprocess.Thecentralcomponentofthisprocessisacascadeofproteolyticenzymescalledcaspases.Theseenzymesparticipateinaseriesofreactionsthataretriggeredinresponsetopro-apoptoticsignalsandresultinthecleavageofproteinsubstrates,causingthedisassemblyofthecell1.

CaspaseshavebeenidentifiedinorganismsrangingfromC.eleganstohumans.Themammaliancaspasesplaydistinctrolesinapoptosisandinflammation.Inapoptosis,caspasesareresponsIBLeforproteolyticcleavagesthatleadtocelldisassembly(effectorcaspases),andareinvolvedinupstreamregulatoryevents(initiatorcaspases).Anactivecaspaseconsistsoftwolargeandtwosmallsubunitsthatformtwoheterodimers,whichassociateinatetramer2-4.Incommonwithotherproteases,caspasesaresynthesizedasprecursorsthatundergoproteolyticmaturation,eitherautocatalyticallyorinacascadebyenzymeswithsimilarspecificity5.

Caspaseenzymesspecificallyrecognizea4or5aminoacidsequenceonthetargetsubstrate,whichnecessarilyincludesanasparticacidresidue.Thisresidueisthetargetforcleavage,whichoccursatthecarbonylendoftheasparticacidresidue6.Caspasescanbedetectedviaimmunoprecipitation,immunoblottingtechniquesusingcaspasespecificantibodies,orbyemployingFluorochromesubstrates,whichbecomefluorescentuponcleavagebythecaspase.
MaterialsRequiredbutNotDelivered·Culturedcellswithmedia

·Reagentstoinduceapoptosis

·15mLpolystyrenecentrifugetubes

·Ambervialsortubesforstorageof150Xconcentrateat-20°C

·600mLgraduatedcylinder

·Microscopeslides

·Hemocytometer

·Centrifuge(400xg)

·37°Cincubator

·Vortexer

·Adjustablevolumepipettorwithdisposabletips

·Deionizedwater

·PBS,pH7.4

·DMSO
ProductInformation
Components
  • FLICAReagent(FAM-VAD-FMK):Onelyophilizedvial
  • 10XWashBuffer:15mL
  • Fixative:6mL
  • PropidiumIodide:1mLat250μg/mL,ready-to-use
  • HoechstStain:1mLat200μg/mL,ready-to-use
DetectionmethodFluorescent
StorageandShippingInformation
StorageConditions·Storeunopenedkitmaterialsat2-8°Cuptotheirexpirationdate.

·ReconstitutedFLICAReagent(150X)shouldbefrozenat-20°Cforupto6months,andmaybethawedtwiceduringthistime.Aliquotintoseparateambertubesifdesired.Protectfromlightatalltimes.

·Storediluted(1X)washbufferupto-2-8°Cfor2weeks.
Applications
ApplicationTheCaspaTagPan-CaspaseInSituAssayKit,Fluoresceinforflowcytometryisafluorescent-basedassayfordetectionofactivecaspasesincellsundergoingapoptosis.
KeyApplications
  • FlowCytometry
  • ActivityAssay
ApplicationNotesTheCHEMICON®CaspaTag™Pan-CaspaseInSituAssayKit,Fluoresceinisafluorescent-basedassayfordetectionofactivecaspasesincellsundergoingapoptosis.Thekitisforresearchuseonly.Notforuseindiagnosticortherapeuticprocedures.

TestPrinciple

CHEMICON®"sCaspaTag™InSituCaspaseDetectionKitsuseanovelapproachtodetectactivecaspases.ThemethodologyisbasedonFluorochromeInhibitorsofCaspases(FLICA).Theinhibitorsarecellpermeableandnon-cytotoxic.Onceinsidethecell,theinhibitorbindscovalentlytotheactivecaspase7.Thiskitusesacarboxyfluorescein-labeledfluoromethylketonepeptideinhibitorofcaspase(FAM-VAD-FMK),whichproducesagreenfluorescence.Whenaddedtoapopulationofcells,theFAM-VAD-FMKprobeenterseachcellandcovalentlybindstoareactivecysteineresiduethatresidesonthelargesubunitoftheactivecaspaseheterodimer,therebyinhibitingfurtherenzymaticactivity.Theboundlabeledreagentisretainedwithinthecell,whileanyunboundreagentwilldiffuseoutofthecellandiswashedaway.Thegreenfluorescentsignalisadirectmeasureoftheamountofactivecaspasepresentinthecellatthetimethereagentwasadded.Cellsthatcontaintheboundlabeledreagentcanbeanalyzedby96-wellplate-basedfluorometry,fluorescencemicroscopy,orflowcytometry.
BIOLOGicalInformation
SpeciesReactivity
  • Mammals
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。