Millipore/APT400 | CaspaTag泛半胱天冬酶原位分析试剂盒，荧光素/APT400/100分析
市场价：
¥11460.00
美元价：
6876.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | APT400 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | CaspaTagPan-CaspaseInSituAssayKit,Fluorescein |
| Overview | Apoptosisisanevolutionarilyconservedformofcellsuicide,whichfollowsaspecializedcellularprocess.Thecentralcomponentofthisprocessisacascadeofproteolyticenzymescalledcaspases.Theseenzymesparticipateinaseriesofreactionsthataretriggeredinresponsetopro-apoptoticsignalsandresultinthecleavageofproteinsubstrates,causingthedisassemblyofthecell(Sleeetal.1999). CaspaseshavebeenidentifiedinorganismsrangingfromC.eleganstohumans.Themammaliancaspasesplaydistinctrolesinapoptosisandinflammation.Inapoptosis,caspasesareresponsIBLeforproteolyticcleavagesthatleadtocelldisassembly(effectorcaspases),andareinvolvedinupstreamregulatoryevents(initiatorcaspases).Anactivecaspaseconsistsoftwolargeandtwosmallsubunitsthatformtwoheterodimerswhichassociateinatetramer(Walkeretal.1994;Wilsonetal.1994;Rotondaetal.1996).Incommonwithotherproteases,caspasesaresynthesizedasprecursorsthatundergoproteolyticmaturation,eitherautocatalyticallyorinacascadebyenzymeswithsimilarspecificity(Kumar1999). Caspaseenzymesspecificallyrecognizea4or5aminoacidsequenceonthetargetsubstratewhichnecessarilyincludesanasparticacidresidue.Thisresidueisthetargetforcleavage,whichoccursatthecarbonylendoftheasparticacidresidue(Thornberryetal.1997).Caspasescanbedetectedviaimmunoprecipitation,immunoblottingtechniquesusingcaspasespecificantibodies,orbyemployingFluorochromesubstrateswhichbecomefluorescentuponcleavagebythecaspase. TestPrinciple: CHEMICON"sInSituCaspaseDetectionKitsuseanovelapproachtodetectactivecaspases.ThemethodologyisbasedonFluorochromeInhibitorsofCaspases(FLICA).Theinhibitorsarecellpermeableandnon-cytotoxic.Onceinsidethecell,theinhibitorbindscovalentlytotheactivecaspase(Ekertetal.1999).Thiskitusesacarboxyfluorescein-labeledfluoromethylketonepeptideinhibitorofcaspase(FAM-VAD-FMK),whichproducesagreenfluorescence.Whenaddedtoapopulationofcells,theFAM-VAD-FMKprobeenterseachcellandcovalentlybindstoareactivecysteineresiduethatresidesonthelargesubunitoftheactivecaspaseheterodimer,therebyinhibitingfurtherenzymaticactivity.Theboundlabeledreagentisretainedwithinthecell,whileanyunboundreagentwilldiffuseoutofthecellandiswashedaway.Thegreenfluorescentsignalisadirectmeasureoftheamountofactivecaspasepresentinthecellatthetimethereagentwasadded.Cellsthatcontaintheboundlabeledreagentcanbeanalyzedby96-wellplate-basedfluorometry,fluorescencemicroscopy,orflowcytometry. Application: TheCHEMICONInSituFLICAPan-CaspaseDetectionKitisafluorescent-basedassayfordetectionofactivecaspasesincellsundergoingapoptosis.Thekitisforresearchuseonly.Notforuseindiagnosticortherapeuticprocedures. |
| MaterialsRequiredbutNotDelivered | ·Culturedcellswithmedia ·Reagentstoinduceapoptosis ·15mLpolystyrenecentrifugetubes ·Ambervialsortubesforstorageof150Xconcentrateat-20°C ·600mLgraduatedcylinder ·Microscopeslides ·Hemocytometer ·Centrifuge(400xg) ·37°Cincubator ·Vortexer ·Adjustablevolumepipettorwithdisposabletips ·Deionizedwater ·PBS,pH7.4 ·DMSO |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Fluorescent |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | ·Storeunopenedkitmaterialsat2-8°Cuptotheirexpirationdate. ·ReconstitutedFLICAReagent(150X)shouldbefrozenat-20ºCforupto6months,andmaybethawedtwiceduringthistime.Aliquotintoseparateambertubesifdesired.Protectfromlightatalltimes. ·Storediluted(1X)washbufferupto-20ºCfor2weeks. |
| Applications | |
|---|---|
| Application | TheInSituFLICAPan-CaspaseDetectionKitforflowcytometryisafluorescent-basedassayfordetectionofactivecaspasesincellsundergoingapoptosis. |
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| BIOLOGicalInformation | |
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| SpeciesReactivity |
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| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases3and7,andtheproteinitselfisprocessedbycaspase8.MutationsinthisgeneareassociatedwithapoptosisdefectsseenintypeIIautoimmunelymphoproliferativesyndrome.Threealternativelysplicedtranscriptvariantsencodingdifferentisoformshavebeendescribedforthisgene. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q92851#IsoformCisproteolyticallyinactive. SIZE:521aminoacids;58951Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya23/17kDa(p23/17)(dependingonthesplicingevents)anda12kDa(p12)subunit(Bysimilarity).Self-associates.InteractswithFADDandCASP8.FoundinaFassignalingcomplexconsistingofFAS,FADD,CASP8andCASP10. TISSUESPECIFICITY:Detectableinmosttissues.Lowestexpressionisseeninbrain,kidney,prostate,testisandcolon. PTM:CleavagebygranzymeBandautocatalyticactivitygeneratethetwoactivesubunits.&PhosphorylateduponDNAdamage,probablybyATMorATR. DISEASE:SwissProt:Q92851#DefectsinCASP10arethecauseofautoimmunelymphoproliferativesyndrometype2A(ALPS2A)[MIM:603909].ALPS2ischaracterizedbyabnormallymphocyteanddendriticcellhomeostasisandimmuneregulatorydefects.&DefectsinCASP10areacauseoffamilialnon-Hodgkinlymphoma(NHL)[MIM:605027].NHLisacancerthatstartsincellsofthelymphsystem,whichispartofthebody"simmunesystem.NHLscanoccuratanyageandareoftenmarkedbyenlargedlymphnodes,feverandweightloss.&DefectsinCASP10areacauseofgastriccancers[MIM:137215]. SIMILARITY:SwissProt:Q92851##BelongstothepeptidaseC14family.&Contains2DED(deatheffector)domains. |
| PhysicochemicalInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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