当前位置：
首页
>
产品中心 >
Functional_antibody >
Millipore/APT161 | Caspase 1 Colorimetric Assay Kit, YVAD/APT161/100 assays
Millipore/APT161 | Caspase 1 Colorimetric Assay Kit, YVAD/APT161/100 assays
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | APT161 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Caspase1ColorimetricAssayKit,YVAD |
| Overview | ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.Chemicon"sICE/Caspase-1ColorimetricProteaseAssayKitsprovideasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceYVAD.Theassayisbasedonspectophotometricdetectionofthechromophorep-nitroanilide(pNA)aftercleavagefromthelabeledsubstrateYVAD-pNA.ThepNAlightemissioncanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat400-or405-nm.ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseinCaspase-1activity. Forresearchuseonly;Notforuseindiagnosticprocedures |
| AlternateNames |
|
| MaterialsRequiredbutNotDelivered | ·Microcentrifugeand1.5mLMicrocentrifugetubes ·37°Cwaterbathorincubator ·Spectrophotometer ·96wellplate |
| ProductInformation | |
|---|---|
| Components |
|
| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Werecommendstoringreagentsat-20°Cuponarrival.(StoreCellLysisBufferand2XReactionBufferanddilutionbufferat4°Cafteropening).Theperformanceofthisproductisguaranteedfor6months. |
| Applications | |
|---|---|
| Application | TheICE/Caspase-1ColorimetricProteaseAssayKitsprovideasimple&convenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceYVAD. |
| KeyApplications |
|
| BIOLOGicalInformation | |
|---|---|
| SpeciesReactivity |
|
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproduce2subunits,largeandsmall,thatdimerizetoformtheactiveenzyme.ThisgenewasidentifiedbyitsABIlitytoproteolyticallycleaveandactivatetheinactiveprecursorofinterleukin-1,acytokineinvolvedintheprocessessuchasinflammation,septicshock,andwoundhealing.Thisgenehasbeenshowntoinducecellapoptosisandmayfunctioninvariousdevelopmentalstages.StudiesofasimilargeneinmousesuggestaroleinthepathogenesisofHuntingtondisease.Alternativesplicingofthisgeneresultsinfivetranscriptvariantsencodingdistinctisoforms. |
| GeneSymbol |
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P29466#ThiolproteasethatcleavesIL-1betabetweenanAspandanAla,releasingthematurecytokinewhichisinvolvedinavarietyofinflammatoryprocesses.Importantfordefenseagainstpathogens.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs).Canalsopromoteapoptosis. SIZE:404aminoacids;45159Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya20kDa(p20)anda10kDa(p10)subunit.Thep20subunitcanalsoformaheterodimerwiththeepsilonisoformwhichthenhasaninhibitoryeffect.Maybeacomponentoftheinflammasome,aproteincomplexwhichalsoincludesPYCARD,CARD8andNALP2andwhosefunctionwouldbetheactivationofproinflammatorycaspases. SUBCELLULARLOCATION:Cytoplasm. TISSUESPECIFICITY:Expressedinlargeramountsinspleenandlung.Detectedinliver,heart,smallintestine,colon,thymus,prostate,skeletalmuscle,peripheralbloodleukocytes,kidneyandtestis.Noexpressioninthebrain. PTM:Thetwosubunitsarederivedfromtheprecursorsequencebyanautocatalyticmechanism. SIMILARITY:SwissProt:P29466##BelongstothepeptidaseC14family.&Contains1CARDdomain. |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
