Millipore/APT139 | Caspase 9比色活性分析试剂盒，LEHD/APT139/25分析
市场价：
¥5500.00
美元价：
3300.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | APT139 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Caspase9ColorimetricActivityAssayKit,LEHD |
| Overview | ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.Chemicon"sCaspase-9ColorimetricActivityAssayKitsprovideasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizetheLEHD.Theassayisbasedonspectophotometricdetectionofthechromophorep-nitroaniline(pNA)aftercleavagefromthelabeledsubstrateLEHD-pNA.ThefreepNAcanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat405nm.ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseincaspase-9activity. |
| AlternateNames |
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| MaterialsRequiredbutNotDelivered | ·Microcentrifugeand1.5mLMicrocentrifugetubes ·37°CWaterbathorIncubator ·SpectrophotometerorMicroplateReader ·96wellMicrotiterPlate |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Storekitmaterialsat-20°Cuptotheirexpirationdate. SpecialPrecautions: ·Afterthawingreagents,useimmediatelyoraliquotandfreezeat-20°Cforlongerstorage.Avoidrepeatedfreeze/thawcycles. ·TheCaspase-9substrateandpNAstandardareespeciallylightsensitive.Maintainthesereagentsinamberorcoveredcontainers. |
| Applications | |
|---|---|
| Application | Caspase-9ColorimetricActivityAssayKitsprovideasimple&convenientmeansforassayingtheactivityofcaspasesthatrecognizetheLEHD. |
| KeyApplications |
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| BIOLOGicalInformation | |
|---|---|
| SpeciesReactivity |
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| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.ThisproteinisprocessedbycaspaseAPAF1;thisstepisthoughttobeoneoftheearliestinthecaspaseactivationcascade.Alternativesplicingresultsintwotranscriptvariantswhichencodedifferentisoforms. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P55211#Isoform2lacksactivityisandominant-negativeinhibitorofcaspase-9. SIZE:416aminoacids;46281Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya35kDa(p35)anda10kDa(p10)subunit.Caspase-9andAPAF1bindtoeachotherviatheirrespectiveNH2-terminalCED-3homologousdomainsinthepresenceofcytochromeCandATP.InteractswiththeinhibitorsBIRC2,BIRC4,BIRC5andBIRC7. TISSUESPECIFICITY:Ubiquitous,withhighestexpressionintheheart,moderateexpressioninliver,skeletalmuscle,andpancreas.Lowlevelsinallothertissues. PTM:CleavagesatAsp-315bygranzymeBandatAsp-330bycaspase-3generatethetwoactivesubunits.Caspase-8and-10canalsobeinvolvedintheseprocessingevents. SIMILARITY:SwissProt:P55211##BelongstothepeptidaseC14family.&Contains1CARDdomain. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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