Millipore/APT131 | Caspase 3比色活性分析试剂盒，DEVD/APT131/25分析
市场价：
¥5500.00
美元价：
3300.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | APT131 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Caspase3ColorimetricActivityAssayKit,DEVD |
| Overview | ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.Chemicon"sCaspase-3ColorimetricActivityAssayKitsprovideasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceDEVD.Theassayisbasedonspectophotometricdetectionofthechromophorep-nitroaniline(pNA)aftercleavagefromthelabeledsubstrateDEVD-pNA.ThefreepNAcanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat405nm.ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseincaspase-3activity. |
| AlternateNames |
|
| MaterialsRequiredbutNotDelivered | ·Microcentrifugeand1.5mLMicrocentrifugetubes ·37°CWaterbathorIncubator ·SpectrophotometerorMicroplateReader ·96wellMicrotiterPlate |
| ProductInformation | |
|---|---|
| Components |
|
| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Storekitmaterialsat-20°Cuptotheirexpirationdate. SpecialPrecautions: ·Afterthawingreagents,useimmediatelyoraliquotandfreezeat-20°Cforlongerstorage.Avoidrepeatedfreeze/thawcycles. ·TheCaspase-3substrateandpNAstandardareespeciallylightsensitive.Maintainthesereagentsinamberorcoveredcontainers. |
| Applications | |
|---|---|
| Application | Caspase-3ColorimetricActivityAssayKitsprovideasimple&convenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceDEVD. |
| KeyApplications |
|
| BIOLOGicalInformation | |
|---|---|
| SpeciesReactivity |
|
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein. |
| GeneSymbol |
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin. SIZE:277aminoacids;31608Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit. SUBCELLULARLOCATION:Cytoplasm. TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem. PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa. SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family. |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
