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当前位置: 首页 > 产品中心 > Functional_antibody > 微孔/CC120 |半胱天冬酶9,重组人活性/CC120/25单位
商品详细微孔/CC120 |半胱天冬酶9,重组人活性/CC120/25单位
微孔/CC120 |半胱天冬酶9,重组人活性/CC120/25单位
微孔/CC120 |半胱天冬酶9,重组人活性/CC120/25单位
商品编号: CC120
品牌: 密理博
市场价: ¥5120.00
美元价: 3072.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberCC120
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionCaspase9,recombinanthumanactive
OverviewCaspase-9isamemberoftheinterleukin-1betaconvertingenzyme(ICE)familyofcysteineproteases.Similartoothercaspases,caspase-9alsoexistsincellsasaninactiveproenzyme.Duringtheinitiationofapoptosistheproenzymeisprocessedataspartateresiduestoformactivecaspase-9.Asoneoftheinitiatorcaspases,activecaspase-9functionstotriggertheactivationofdownstreameffectorcaspasesandfurtherleadtodisassemblyofcellstructures.

Thepartiallypurifiedactiverecombinanthumancaspase-9withfull-lengthHIS-6tagwasexpressedinE.coli.Theexpressedcaspase-9spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicofthenativeenzyme.Incombinationwithcaspase-9activityassays,theactiverecombinantcaspase-9isusefulinBIOLOGicalscreeningofcaspaseinhibitors.Therecombinantenzymecanalsobeusedasapositivecontrolincaspase-9assays.Caspase9isknowntohavestrongreactivitywithothersequencepeptides,especiallyDEVD-pNA.
AlternateNames
  • APAF-3
  • ICE-LAP6
  • Mch-6
ProductInformation
PresentationLyophilized.Reconstitutewith25μlofPBS+15%glycerol.
StorageandShippingInformation
StorageConditionsMaintainat-70°Cinundilutedaliquotsfor3-6monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.
Applications
KeyApplications
  • ActivityAssay
ApplicationNotesEnzymeispreparedasanactivitystandardonly,anyotherapplicationnottested.Caspase-9issubjecttoauto-activationthusactivecaspasesubunitsandpartiallyactiveintermediateswillbeseen.Bandsofapproximately45kDa(pro),35kDa(partiallyactive)and18kDa+12kDa(largeandsmallsubunits)maybeseenbywesternblotdependingupontheantibodyused.Thesmallsubunitisextremelydifficulttodetectinmostwesterns.

Activation:incubate1-5μLofstockinPBSpH7.5(100mMHEPEScanbeusedaswell)plus10mMATPand5mMDTT(addedjustbeforeuse)for30minutesat37°C.
BiologicalInformation
Purity10-20%caspase-9proteinbygelanalysis.AlsocontainsE.coliproteins.Approximately,0.8-2μgtotalprotein/unitofactivity.
SourceHuman
SpecificActivityOneunitoftherecombinantcaspase-9istheenzymeactivitythatcleaves1nmolofthecaspasesubstrateLEHD-pNA(pNA:pnitroanaline)perhourat37°Catsaturatedsubstrateconcentrations.
EntrezGeneNumber
EntrezGeneSummaryThisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.ThisproteinisprocessedbycaspaseAPAF1;thisstepisthoughttobeoneoftheearliestinthecaspaseactivationcascade.Alternativesplicingresultsintwotranscriptvariantswhichencodedifferentisoforms.
GeneSymbol
  • CASP9
  • CASP-9
  • ICE-LAP6
  • MCH6
  • APAF3
  • CASPASE-9c
  • OTTHUMP00000044594
  • APAF-3
  • EC3.4.22.62[Contains:Caspase-9subunitp35
  • Caspase-9subunitp10].
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:P55211#Isoform2lacksactivityisandominant-negativeinhibitorofcaspase-9.
SIZE:416aminoacids;46281Da
SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya35kDa(p35)anda10kDa(p10)subunit.Caspase-9andAPAF1bindtoeachotherviatheirrespectiveNH2-terminalCED-3homologousdomainsinthepresenceofcytochromeCandATP.InteractswiththeinhibitorsBIRC2,BIRC4,BIRC5andBIRC7.
TISSUESPECIFICITY:Ubiquitous,withhighestexpressionintheheart,moderateexpressioninliver,skeletalmuscle,andpancreas.Lowlevelsinallothertissues.
PTM:CleavagesatAsp-315bygranzymeBandatAsp-330bycaspase-3generatethetwoactivesubunits.Caspase-8and-10canalsobeinvolvedintheseprocessingevents.
SIMILARITY:SwissProt:P55211##BelongstothepeptidaseC14family.&Contains1CARDdomain.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。