Millipore/CC119 |人半胱天冬酶3，重组人活性/CC119/25单位
市场价：
¥4300.00
美元价：
2580.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | CC119 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | HumanCaspase3,recombinanthumanactive |
| Overview | Specificity:Caspase-3(alsoknownasCPP32,Yamaandapopain)isamemberoftheinterleukin-1betaconvertingenzyme(ICE)familyofcysteineproteases.Caspase-3existsincellsasaninactive32kDaproenzyme.Duringapoptosisprocaspase-3isprocessedataspartateresiduesbyself-proteolysisand/orcleavagebyupstreamcaspases,suchascaspase-6(Mch2),caspase-8(FLICE)granzymeB.Theprocessedformofcaspase-3consistsoflarge(17kDa)andsmall(12kDa)subunitswhichassociatetoformtheactiveenzyme.Theactivecaspase-3hasbeenshowninvolvingintheproteolysisofseveralimportantmolecules,suchaspoly(ADP-ribose)polymerase(PARP),thesterolregulatoryelementbindingproteins(SREBPs),focaladhesionkinase(FAK),andothers. Thepartiallypurified*activerecombinanthumancaspase-3proteinwithfull-lengthHis-6tagwasexpressedinE.coli.TheexpressedCaspase-3spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicofthenativeenzyme.Therecombinantcaspase-3preferentiallycleavescaspase-3substrates(e.g.,DEVD-AFCorDEVD-pNA).Incombinationwithcaspase-3activityassays,theactiverecombinantcaspase-3isveryusefulintheBIOLOGicalscreeningofcaspaseinhibitors.Therecombinantenzymecanalsobeusedasapositivecontrolincaspaseassaysorindeterminingthespecificityofcaspasesubstrates. *10-25%caspase-3proteinbygelanalysis,alsocontainsE.Coliproteins.Approximately,0.8-2μgtotalprotein/unitofactivity.GenBank#U13737. |
| ProductInformation | |
|---|---|
| Presentation | Lyophilized.Reconstituteto1unit/μLwithPBScontaining15%glycerol. Partiallypurifiedrecombinantcaspase3withafulllengthHIS-6-tag.Approximately10-20%caspase3protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainunopenedandrehydratedproductat-70°Cinundiluted,freeze-thawprotected,tightlysealedaliquots.Avoidrepeatedfreeze/thawcycles.Productstablefor6monthsfromdateofpurchasedifhandledappropriately. |
| BiologicalInformation | |
|---|---|
| Purity | Partiallypurifiedrecombinantcaspase3withafulllengthHIS-6-tag.Approximately10-20%caspase3protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity. |
| SpecificActivity | Oneunitoftherecombinantcaspase-3istheenzymeactivitythatcleaves1nmolofthecaspasesubstrateDEVD-pNA(pNA:pnitroanaline)perhourat37°Catsaturatedsubstrateconcentrations. |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein. |
| GeneSymbol |
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin. SIZE:277aminoacids;31608Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit. SUBCELLULARLOCATION:Cytoplasm. TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem. PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa. SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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