4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/04-573 | Anti-Caspase-8 Antibody, rabbit monoclonal/04-573/100 µL
商品详细Millipore/04-573 | Anti-Caspase-8 Antibody, rabbit monoclonal/04-573/100 µL
Millipore/04-573 | Anti-Caspase-8 Antibody, rabbit monoclonal/04-573/100 µL
Millipore/04-573 | Anti-Caspase-8 Antibody, rabbit monoclonal/04-573/100 µL
商品编号: 04-573
品牌: 密理博
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumber04-573
BrandFamilyUpstate
TradeName
  • Upstate
DescriptionAnti-Caspase-8Antibody,rabbitmonoclonal
BackgroundInformationCaspase-8(MACH/FLICE/Mch5)isamemberofafamilyofcysteine-requiringaspartateproteasesthatparticipateintheintracellularsignalingcascadeleADIngtoapoptosis,orprogrammedcelldeath(1-4).Caspase-8isrecruitedtodeath-inducingsignalingcomplex(DISC)CD95(Fas/APO-1)andtumornecrosisfactorreceptor1(TNFR1)andissubsequentlyactivatedbyproteolyticcleavageintotworesidues,p10andp18(1-4).Activecaspase-8,thefirstinthecaspasesignalingcascade,signalsdownstreamcaspasestoeventuallyinduceapoptosis.
ProductInformation
FormatPurified
PresentationBuffer:50mMTris-Glycine(pH7.4),0.15MNaCl,40%Glycerol,0.01%sodiumazideand0.05%BSA.
StorageandShippingInformation
StorageConditionsStoreat-20°C.Stablefor12monthsfromdateofreceipt.
Applications
ApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.Anti-Caspase-8AntibodydetectslevelofCaspase-8&hasbeenpublished&validatedforuseinIP,WB,FC.
KeyApplications
  • Immunoprecipitation
  • WesternBlotting
  • FlowCytometry
ApplicationNotesWB:1:500
FlowCytometry:1:20
IP:1:40
BIOLOGicalInformation
ImmunogenAsyntheticpeptidecorrespondingtoN-terminalresiduesofhumanCaspase-8subunitp18.Theantibodyshouldrecognizebothpro-form(55kDa)andp18cleaved-formofCaspase-8.
CloneE7
HostRabbit
SpecificityRecognizesHuman,RatCaspase-8.
SpeciesReactivity
  • Human
  • Rat
SpeciesReactivityNoteHuman,Rat(positivecontrol:Helacelllysate)
AntibodyTypeMonoclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryThisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymescomposedofaprodomain,alargeproteasesubunit,andasmallproteasesubunit.Activationofcaspasesrequiresproteolyticprocessingatconservedinternalasparticresiduestogenerateaheterodimericenzymeconsistingofthelargeandsmallsubunits.ThisproteinisinvolvedintheprogrammedcelldeathinducedbyFasandvariousapoptoticstimuli.TheN-terminalFADD-likedeatheffectordomainofthisproteinsuggeststhatitmayinteractwithFas-interactingproteinFADD.ThisproteinwasdetectedintheinsolublefractionoftheaffectedbrainregionfromHuntingtondiseasepatientsbutnotinthosefromnormalcontrols,whichimplicatedtheroleinneurodegenerativediseases.Manyalternativelysplicedtranscriptvariantsencodingdifferentisoformshavebeendescribed,althoughnotallvariantshavehadtheirfull-lengthsequencesdetermined.
GeneSymbol
  • CASP8
  • MCH5
  • MGC78473
  • CASP-8
  • MACH
  • ALPS2B
  • FLICE
  • procaspase-8
  • CAP4
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:Q14790#MostupstreamproteaseoftheactivationcascadeofcaspasesresponsIBLefortheTNFRSF6/FASmediatedandTNFRSF1Ainducedcelldeath.BindingtotheadaptermoleculeFADDrecruitsittoeitherreceptor.Theresultingaggregatecalleddeath-inducingsignalingcomplex(DISC)performsCASP8proteolyticactivation.TheactivedimericenzymeisthenliberatedfromtheDISCandfreetoactivatedownstreamapoptoticproteases.ProteolyticfragmentsoftheN-terminalpropeptide(termedCAP3,CAP5andCAP6)arelikelyretainedintheDISC.CleavesandactivatesCASP3,CASP4,CASP6,CASP7,CASP9andCASP10.MayparticipateintheGZMBapoptoticpathways.CleavesADPRT.Hydrolyzesthesmall-moleculesubstrate,Ac-Asp-Glu-Val-Asp--AMC.LikelytargetforthecowpoxvirusCRMAdeathinhibitoryprotein.Isoforms5,6,7and8lackthecatalyticsiteandmayinterferewiththepro-apoptoticactivityofthecomplex.
SIZE:479aminoacids;55391Da
SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya18kDa(p18)anda10kDa(p10)subunit.InteractswithFADD,CFLARandPEA15.Isoform9interactsattheendoplasmicreticulumwithacomplexcontainingBCAP31,BAP29,BCL2and/orBCL2L1.
SUBCELLULARLOCATION:Cytoplasm.
TISSUESPECIFICITY:Isoforms1,5and7areexpressedinawidevarietyoftissues.Highestexpressioninperipheralbloodleukocytes,spleen,thymus,andliver.Barelydetectableinbrain,testis,andskeletalmuscle.DOMAIN:SwissProt:Q14790Isoform9containsaN-terminalextensionthatisrequiredforinteractionwiththeBCAP31complex.
PTM:Generationofthesubunitsrequiresassociationwiththedeath-inducingsignalingcomplex(DISC),whereasadditionalprocessingislikelyduetotheautocatalyticactivityoftheactivatedprotease.GZMBandCASP10canbeinvolvedintheseprocessingevents.&PhosphorylateduponDNAdamage,probablybyATMorATR.
DISEASE:SwissProt:Q14790#DefectsinCASP8arethecauseofcaspase-8deficiency(CASP8D)[MIM:607271].CASP8Disadisorderresemblingautoimmunelymphoproliferativesyndrome(ALPS).Itischaracterizedbylymphadenopathy,splenomegaly,anddefectiveCD95-inducedapoptosisofperipheralbloodlymphocytes(PBLs).ItleadstodefectsinactivationofT-lymphocytes,B-lymphocytes,andnaturalkillercellsleadingtoimmunodeficiencycharacterizedbyrecurrentsinopulmonaryandherpessimplexvirusinfectionsandpoorresponsestoimmunization.
SIMILARITY:BelongstothepeptidaseC14family.&Contains2DED(deatheffector)domains.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。