密理博/04-1090 |抗半胱天冬酶-3抗体，克隆E87，兔单克隆/04-1090/100µ；L
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 04-1090 |
| Description | Anti-Caspase-3Antibody,cloneE87,rabbitmonoclonal |
| AlternateNames |
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| BackgroundInformation | Caspase-3(CPP32,Apopain)isthemostextensivelystudiedapoptoticprotein.Caspase-3issynthesizedasaninactiveproenzyme(32kDa)thatisprocessedincellsundergoingapoptosisbyself-proteolysisand/orcleavagebyanotherupstreamprotease.Theprocessedformofcaspase-3consistsoflarge(17kDa)andsmall(12kDa)subunitswhichassociatetoformanactiveenzyme.Theactivecaspase-3proteolyticallycleavesandactivatesothercaspases,aswellasrelevanttargetsinthecellssuchasPARPandDFF. |
| ProductInformation | |
|---|---|
| Format | Unpurified |
| Control |
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| Presentation | RabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20ºCfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Syntheticpeptidecorrespondingtoresiduesinp17subunitofhumanCaspase-3. |
| Epitope | p17subunit |
| Clone | E87 |
| Host | Rabbit |
| Specificity | Theantibodyshouldrecognizebothpro-form(35kDa)andp17cleaved-formofCaspase-3. |
| Isotype | IgG |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein.[providedbyRefSeq]. |
| GeneSymbol |
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| PurificationMethod | Unpurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp-|-Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin. CATALYTICACTIVITY:StrictrequirementforanAspresidueatpositionsP1andP4.IthasapreferredcleavagesequenceofAsp-Xaa-Xaa-Asp-|-withahydrophobicamino-acidresidueatP2andahydrophilicamino-acidresidueatP3,althoughValorAlaarealsoacceptedatthisposition. ENZYMEREGULATION:Inhibitedbyisatinsulfonamides. SUBUNITSTRUCTURE:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit. SubcellularlocationCytoplasm. TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem. PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa. S-nitrosylatedonitscatalyticsitecysteineinunstimulatedhumancelllinesanddenitrosylateduponactivationoftheFasapoptoticpathway,associatedwithanincreaseinintracellularcaspaseactivity.Fasthereforeactivatescaspase-3notonlybyinducingthecleavageofthecaspasezymogentoitsactivesubunits,butalsobystimulatingthedenitrosylationofitsactivesitethiol. SEQUENCESIMILARITY:BelongstothepeptidaseC14Afamily. |
| MolecularWeight | ~35kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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