密理博/MAB5304 |抗谷氨酸抗体/MAB5304/100µ；L
市场价：
¥7180.00
美元价：
4308.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB5304 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-GlutamateAntibody |
| ProductInformation | |
|---|---|
| Format | Purified |
| Presentation | LiquidinPBScontaining10mMsodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat2-8°Cinundilutedaliquotsforupto6monthsafterdate. |
| Applications | |
|---|---|
| Application | Anti-GlutamateAntibodydetectslevelofGlutamate&hasbeenpublished&validatedforuseinIH. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistry:1:2,500-1:10,000usingfreefloatingsectionsbythePAPtechniqueonrathippocampus. Optimalworkingdilutionsmustbedeterminedbyenduser. PROTOCOLforGlutamateDetectionbyImmunohisto/cytochemistry.Exampleforaratbrain. 1.SOLUTIONSTOBEPREPARED-Solutionmustbepreparedasneeded. SolutionA:0.1Mcacodylate,10g/LsodiummetABIsulfite,pH6.2. SolutionB:0.1Mcacodylate,10g/Lsodiummetabisulfite,3-5%glutaraldehyde,pH7.5. 2.RATPERFUSION-TheratisanaesthetizedwithsodiumpentobarbitalorNembutalandperfusedintracardiallythroughtheaortausingapumpwithSolutionA(30mL):150-300mL/min,SolutionB(500mL):150-300mL/min. 3.POSTFIXATION:15to30minutesinSolutionB,then4softwashesin0.05MTriswith8.5g/Lsodiummetabisulfite,pH7.5(SolutionC). 4.TISSUESECTIONING:Vibratomeorcryostatsectionscanbeused. 5.REDUCTIONSTEP:SectionsarereducedwithSolutionCcontaining0.1Msodiumborohydridefor10minutes.Thesectionsarewashed4timesinsolutionCwithoutsodiumborohydride. 6.APPLICATIONOFGLUTAMATEANTIBODY:Useafinaldilutionof1:2,500-1:10,000inSolutionCcontaining0.1%TritonX100and2%non-specificserum.Incubate12sectionsper2mLdilutedantibodyovernight,+2-8°C.Thenwashthesectionsthreetimesfor10minuteseachinSolutionC.(Notethattheantibodymaybeusableatahigherdilution.Thisshouldbeexploredtominimizethepossibilityofhighbackground.Additionally,notethatachangeinthebufferingsystemasindicatedintheprotocolmaychangethebackgroundandantibodyrecognition).ThespecificreactionisthenrevealedbyPAPprocedure. 7.SECONDANTIBODY:Incubatethesectionswitha1:50to1:200dilutionofgoatanti-mouseinSolutionBcontaining1%non-specificserumforeither3hrsat20°Cor1-2hrat37°C.Thenwashthesections,3times,for10minuteseachwithSolutionC. 8.PAP:Incubatethesectionswiththeappropriatedilutionofperoxidaseanti-peroxidase(forfreefloatingmethod)inSolutionCfor1-2hoursat37°C.Thenwashsections3timesfor10mineachinsolutionC. 9.VISUALIZATION:Theantigen-antibodycomplexesarevisualizedusingDAB-4-HCl(25mg/100mL)(orotherchromogen)in0.05MTrisandfiltrated;0.05%hydrogenperoxideisadded.Incubatethesectionsfor10minutesatroomtemp.Stopthereactionbytransferringthesectionsto5mL0.05MTris.Mountsectionsonchrome-alumcoatedslides.Dryovernightat37°C.Rehydratesectionsusingconventionalhistologicalprocedures.Coverslipusingrapidmountingmedia. Forresearchuseonly;notforuseasadiagnostic. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Glutamate-Glutaraldehyde-BSA. |
| Host | Mouse |
| Specificity | Glutamate Thecross-reactivitiesweredeterminedusinganELISAtestbycompetitionexperimentswiththefollowingcompounds: CompoundCross-reactivity Glutamate-G-BSA1 Aspartate-G-BSA1/100,000 GABA-G-BSA1/100,000 Glutamate1/100,000 Abbreviations: (G)Glutaraldehyde (BSA)BovineSerumAlbumin NOTE:AntibodyreactivityREQUIRESglutaraldehydefixationthussomeglutaraldehyde(0.5%-2.0%)needstobeincludedinthetissuefixationprocedureinorderfortheproperreactivity. |
| Isotype | IgM |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
