密理博/AB175 |抗GABA抗体/AB175/50µ；L
市场价：
¥7400.00
美元价：
4440.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | AB175 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-GABAAntibody |
| BackgroundInformation | GABA(g-aminobutyricacid)isthemajorinhibitoryneurotransmitterinthecentralnervoussystemthatisinvolvedinthepathogenesisofcertainneurologicalandpsychiatricdisorders.GABAisproducedfromglutamicacidinareactioncatalyzedbyglutamicaciddecarboxylase.GABAinteractswiththeGABAAandGABABreceptors,whicharewidelydistributedthroughoutthenervoussystemandinavarietyofcelltypes.Theimmunolocalization,neuronaldistribution,andvesicularcompartmentalizationofneurotransmittermoleculesandtheirprecursorsareimportantforunderstandingthemodulation,regulation,andfunctionofneurotransmittersinthenervoussystem. |
| ProductInformation | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Purifiedguineapigpolyclonalwith0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponreceiptandpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| Applications | |
|---|---|
| Application | ThisAnti-GABAAntibodyisvalidatedforuseinImmunohistochemistry(Paraffin)andEnzymeImmunoassay(ELISA)forthedetectionofGABA. |
| KeyApplications |
|
| ApplicationNotes | ImmunohistochemistryAnalysis:A1:500dilutionfromarepresentativelotdetectedGABAinratbraintissue. ELISAAnalysis:ArepresentativelotfromanindependentlaboratorydetectedGABAinacompetitiveELISA. ImmunohistochemistryProtocol: Tissuesfixedwith4%paraformaldehydeand0-0.5%glutaraldehydegivesgoodresults.Glutaraldehydeisrequiredforantibodyreactivity. 1)Tissueisfixedwith4%paraformaldehyde,0-0.5%glutaraldehyde,0.5%potassiumdichromatein0.1MphosphatebufferatpH6.5. 2)Tissueispost-fixedovernight,vibratomesectionedin50mmandincubatedin0.05MTrisbuffer,pH6.5forthreehours. 3)Sectionsareincubatedfor18-24hoursinAB175dilutedinPBScontaining0.1%sodiumazide,0.2%TritonX-100and1%normalgoatserum. 4)FluoresceinconjugatedantibodyorABCsystemmaybeusedasthesecondaryreagent. Note:Withoutcolchicinepretreatmentwell-stainedcellbodiesarevisIBLeinthecerebralcortex,cerebrallarcortex,superiorcolliculusandsomebrainstemraphe.Withcolchicinepretreatment,additionalcellbodystainingispresentintheinterpeduncularnucleusandthedorsalcolumnnuclei. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | BSA-conjugatedGABA-Glutaraldehyde |
| Concentration | Variable |
| Host | GuineaPig |
| Specificity | RecognizesGABA.Stainingwasblockedbypreabsorbingwith100μMGABAconjugatedtoglutaraldehyde.500μMofsimilarconjugationsofglutamicacid,glutamateandtaurinefailedtoblockstaining. |
| SpeciesReactivity |
|
| SpeciesReactivityNote | DemonstratedtoreactwithRat.GABAisaneurotransmittersynthesizedinallspecies,andisthereforeexpectedtoreacttoallspecies. |
| AntibodyType | PolyclonalAntibody |
| PurificationMethod | Depletedserum |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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