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Millipore/MAB5300 | Anti-Dopamine Antibody, clone K56A/MAB5300/100 µL
Millipore/MAB5300 | Anti-Dopamine Antibody, clone K56A/MAB5300/100 µL
市场价：
¥7180.00
美元价：
4308.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB5300 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-DopamineAntibody,cloneK56A |
| ProductInformation | |
|---|---|
| Format | Purified |
| Presentation | Purifiedimmunoglobulin.Liquidcontaining50%glycerol. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat-20°Cinundilutedaliquotsforupto6monthsafterdateofreceipt. |
| Applications | |
|---|---|
| Application | Anti-DopamineAntibody,cloneK56AdetectslevelofDopamine&hasbeenpublished&validatedforuseinIH. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistry:1:500-1:2,500usingfreefloatingsectionsbythePAPtechniqueonratdopaminergicareas. Optimalworkingdilutionsmustbedeterminedbyenduser. PROTOCOLforDopamineDetectionbyImmunohisto/cytochemistry.Exampleforaratbrain. 1.SOLUTIONSTOBEPREPARED-Solutionmustbepreparedasneeded. SolutionA:0.1Mcacodylate,10g/LsodiummetABIsulfite,pH6.2. SolutionB:0.1Mcacodylate,10g/Lsodiummetabisulfite,3-5%glutaraldehyde,pH7.5. 2.RATPERFUSION-TheratisanaesthetizedwithsodiumpentobarbitalorNembutalandperfusedintracardiallythroughtheaortausingapumpwithSolutionA(30mL):150-300mL/min,SolutionB(500mL):150-300mL/min. 3.POSTFIXATION:15to30minutesinSolutionB,then4softwashesin0.05MTriswith8.5g/Lsodiummetabisulfite,pH7.5(SolutionC). 4.TISSUESECTIONING:Vibratomeorcryostatsectionscanbeused. 5.REDUCTIONSTEP:SectionsarereducedwithSolutionCcontaining0.1Msodiumborohydridefor10minutes.Thesectionsarewashed4timesinsolutionCwithoutsodiumborohydride. 6.APPLICATIONOFDOPAMINEANTIBODY:Useafinaldilutionof1:2,500-1:10,000inSolutionCcontaining0.1%TritonX100and2%non-specificserum.Incubate12sectionsper2mLdilutedantibodyovernight,+4°C.Thenwashthesectionsthreetimesfor10minuteseachinSolutionC.(Notethattheantibodymaybeusableatahigherdilution.Thisshouldbeexploredtominimizethepossibilityofhighbackground.Additionally,notethatachangeinthebufferingsystemasindicatedintheprotocolmaychangethebackgroundandantibodyrecognition).ThespecificreactionisthenrevealedbyPAPprocedure. 6.SECONDANTIBODY:Incubatethesectionswitha1:50to1:200dilutionofgoatanti-rabbitinSolutionBcontaining1%non-specificserumforeither3hrsat20°Cor2hrat37°C.Thenwashthesections,3times,for10minuteseachwithSolutionB. 7.PAP:Incubatethesectionswiththeappropriatedilutionofperoxidaseanti-peroxidase(forfreefloatingmethod)inSolutionBcontaining1%non-specificserumfor1-2hoursat37°C.Thenwashsections3timesfor10mineachinsolutionB. 8.VISUALIZATION:Theantigen-antibodycomplexesarevisualizedusingDAB-4-HCl(25mg/100mL)(orotherchromogen)in0.05MTrisandfiltrated;0.05%hydrogenperoxideisadded.Incubatethesectionsfor10minutesatroomtemp.Stopthereactionbytransferringthesectionsto5mL0.05MTris.WashtissuewithsolutionDusing2,10minwashes.Mountsectionsonchrome-alumcoatedslides.Dryovernightat37°C.Rehydratesectionsusingconventionalhistologicalprocedures.Coverslipusingrapidmountingmedia. Forresearchuseonly;notforuseasadiagnostic. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Dopamine-Gluteraldehyde-BSA. |
| Clone | K56A |
| Host | Mouse |
| Specificity | Dopamine. Thecross-reactivitiesweredeterminedusinganELISAtestbycompetitionexperimentswiththefollowingcompounds: CompoundCross-reactivity Dopamine-G-BSA1 L-DOPA-G-BSA1/10,000 Tyrosine-G-BSA1/36,000 Tyramine-G-BSA1/>50,000 Noradrenaline-G-BSA1/>50,000 Octopamine-G-BSA1/>50,000 Adrenaline-G-BSA1/>50,000 Dopamine1/>50,000 Abbreviations: (G)Glutaraldehyde (BSA)BovineSerumAlbumin |
| Isotype | IgG |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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