Millipore/MAB5292 |抗多巴胺D1B受体抗体，克隆SG4-D1B/MAB5292/100µ；G
市场价：
¥6580.00
美元价：
3948.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB5292 |
| Replaces | AB1791P |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Anti-DopamineD1BReceptorAntibody,cloneSG4-D1B |
| AlternateNames |
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| ProductInformation | |
|---|---|
| Format | Purified |
| Presentation | Purifiedimmunoglobulin.Liquidin0.02MPhosphatebuffer,0.25MNaCl,pH7.6with0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat2-8°Cinundilutedaliquotsforupto6monthsafterdateofreceipt. |
| Applications | |
|---|---|
| Application | ThisAnti-DopamineD1BReceptorAntibody,cloneSG4-D1BisvalidatedforuseinIH,WBforthedetectionofDopamineD1BReceptor. |
| KeyApplications |
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| ApplicationNotes | Westernblot:1:250-1:500onmousebrainlysate(unboiledsample) Immunohistochemistry Optimalworkingdilutionsmustbedeterminedbytheenduser. SUGGESTEDWESTERNBLOTPROTOCOL Solutions 10XTransferbuffer1liter Tris115.3gm Glycine24.2gm [SDS4gm(maybeomitted)] Fora1Xworkingsolution:1liter mix700mLwater 200mL MeOH100mL 10XTransfer TBSTstock1liter1M TrispH7.510mL 5MNaCl30mL 10%Tween-205Ml 1.RunSDS-PAGEgelasdesired. 2.SoakPVDFmembrane(ImmobilonPfromMillipore)in100%methanolfor1-2minutestowetthemembrane. 3.Soakmembraneintransferbuffer(seebelow)untilreadytosetupblot(>15min)transfer. 4.Assemblegel-transfersandwichwiththeblottowardthepositiveelectrode. 5.Transferproteinfromgeltomembraneat100voltsfor1-2hoursforsmallmolecularweightproteinsor65mAfor16hours(overnight)forcompletetransferofhighermolecularweightproteins. 6.StainthetransferredbandswithChemiconBLOT-FastStain(CatalogNumber2076). 7.Destainwithdeionizedwater. 8.Blockwith5%non-fatmilk(MarvelorCarnation)inwater,overnightat4°C.Thenon-fatmilkshouldbedissolvedfreshly,centrifuged10,000rpmfor10min,andfilteredthroughglassfilter(GelmanAcrodisc). 9.Incubationwithfirstantibodyovernightwithrockingat4°Cinblockingsolution.Optimalworkingdilutionsandincubationtimewillneedtobedeterminedbytheenduser. 10.Washatleast3x5min.withTBST.Fromthisstage,azideshouldbeomitted. 11.Incubationwiththesecondaryantibody(HRP-conjugatedgoatanti-mouseantibody,forexampleChemiconCatalogNumberAP124P,dilutedappropriately)1hatroomtemperature. 12.Washatleast3x5min.withTBST. 13.PerformECLwithcommercialkit(femtoLUCENT,ChemiconCatalogNumber2078). |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | RecombinantratD1bReceptor,carboxyterminus. |
| Clone | SG4-D1B |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | DopamineReceptorD1b(D5). |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgeneencodestheD5subtypeofthedopaminereceptor.TheD5subtypeisaG-proteincoupledreceptorwhichstimulatesadenylylcyclase.Thisreceptorisexpressedinneuronsinthelimbicregionsofthebrain.Ithasa10-foldhigheraffinityfordopaminethantheD1subtype.Pseudogenesrelatedtothisgeneresideonchromosomes1and2. |
| GeneSymbol |
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| Non-ReactiveSpecies |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P21918#Thisisoneofthefivetypes(D1toD5)ofreceptorsfordopamine.TheactivityofthisreceptorismediatedbyGproteinswhichactivateadenylylcyclase. SIZE:477aminoacids;52951Da SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein. TISSUESPECIFICITY:Neuron-specific,localizedprimarilywithinlimbicregionsofthebrain. DISEASE:SwissProt:P21918#DefectsinDRD5maybeacauseofschizophrenia,butnoproofhasyetbeenfound.&DefectsinDRD5areacauseofblepharospasm[MIM:606798].Itisaprimaryfocaldystoniaaffectingtheorbicularisoculimuscles,usuallybeginninginmiddleage.Initialsymptomsincludeeyeirritationandfrequentblinking,progressingtoinvoluntaryspasmsofeyelidclosure.Inseverecases,thiscanleadtofunctionalblindness. SIMILARITY:SwissProt:P21918##BelongstotheG-proteincoupledreceptor1family. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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