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Millipore/AB5522 | Anti-Acetylcholine Antibody/AB5522/100 µL
Millipore/AB5522 | Anti-Acetylcholine Antibody/AB5522/100 µL
市场价：
¥7400.00
美元价：
4440.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | AB5522 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-AcetylcholineAntibody |
| ProductInformation | |
|---|---|
| Format | Serum |
| Presentation | Rabbitantiserum.Liquidwith0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat-20°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.Avoidrepeatedfreeze/thawcycles. Duringshipment,smallvolumesofantibodywilloccasionallybecomeentrappedinthesealoftheproductvial.Forantibodieswithvolumesof200μlorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer"scap. |
| Applications | |
|---|---|
| Application | Anti-AcetylcholineAntibodyisanantibodyagainstAcetylcholineforuseinIH. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistry:1:250-1:2500byPAP(seesuggestedprotocolbelow). Optimalworkingdilutionsmustbedeterminedbytheenduser. PROTOCOLforAcetylcholineDetectionbyImmunohisto/cytochemistry.Exampleforaratbrain. 1.SOLUTIONSTOBEPREPARED-Solutionmustbepreparedasneeded. SolutionA:0.5Mglutaraldehyde,0.1M2-nitrobenzylalcohol,10g/LsodiummetABIsulfite,0.1Mcacodylate,0.01Msodiumbromide,pH10.7. SometimesaprecipitatemayformwhilemakingsolutionA.Toavoidthis,firstprepareamixturewith1.0Mglutaraldehydeand0.2M2-nitrobenzylalcohol.Prepareasecondmixturewith20g/Lsodiummetabisulfiteand0.2Mcacodylate;addsodiumbromidetoobtainpH10.7.Thencombinethetwomixturesinequalpartswhilestirring. SolutionB:0.05MTris,10.0g/Lsodiummetabisulfite,pH7.4.(*) 2.RATPERFUSION-TheratisanaesthetizedwithsodiumpentobarbitalorNembutalandperfusedintracardiallythroughtheaortausingapumpwithSolutionA(200-300mL/min). 3.POSTFIXATION:2hoursin0.5Mglutaraldehydesolution,(pH7.5)withoutthe2-nitrobenzylalcohol,then4softwashesinsolutionB. 4.TISSUESECTIONING:Vibratomeorcryostatsectionscanbeused. Thesectionsarewashed4timesinsolutionB,andincubatedfor1hourat37°CinsolutionBcontaining0.2%TritonX100,plus1%nonspecificserum. 5.APPLICATIONOFACETYLCHOLINEANTIBODY:Useafinaldilutionof1:500-1:2,500inSolutionBcontaining0.2%TritonX100and1%non-specificserum.Incubate12sectionsper2mLdilutedantibodyovernight,+4°C.Thenwashthesectionsthreetimesfor10minuteseachinSolutionB.(Notethattheantibodymaybeusableatahigherdilution.Thisshouldbeexploredtominimizethepossibilityofhighbackground.Additionally,notethatachangeinthebufferingsystemasindicatedintheprotocolmaychangethebackgroundandantibodyrecognition).ThespecificreactionisthenrevealedbyPAPprocedure. 6.SECONDANTIBODY:Incubatethesectionswitha1:50to1:200dilutionofgoatanti-rabbitinSolutionBcontaining1%non-specificserumforeither3hrsat20°Cor2hrat37°C.Thenwashthesections,3times,for10minuteseachwithSolutionB. 7.PAP:Incubatethesectionswiththeappropriatedilutionofperoxidaseanti-peroxidase(forfreefloatingmethod)inSolutionBcontaining1%non-specificserumfor1-2hoursat37°C.Thenwashsections3timesfor10mineachinsolutionB. 8.VISUALIZATION:Theantigen-antibodycomplexesarevisualizedusingDAB-4-HCl(25mg/100mL)(orotherchromogen)in0.05MTrisandfiltrated;0.05%hydrogenperoxideisadded.Incubatethesectionsfor10minutesatroomtemp.Stopthereactionbytransferringthesectionsto5mL0.05MTris.WashtissuewithsolutionDusing2,10minwashes.Mountsectionsonchrome-alumcoatedslides.Dryovernightat37°C.Rehydratesectionsusingconventionalhistologicalprocedures.Coverslipusingrapidmountingmedia. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Acetylcholine-Glutaricanhydride-PL |
| Host | Rabbit |
| Specificity | Acetylcholine Thecross-reactivitiesweredeterminedusingeitherELISAorRIAtechniques,atconcentration/unconjugatedorconjugatedaminoacidconcentrationathalfdisplacementwiththefollowingcompounds: CompoundCross-reactivity Choline-GA-PL1 Phosphatidylcholine1/>10,000 Choline1/>10,000 Acetylcholine1/>10,000 Choline-allylalcohol-G-BSA1/1.6 Theantiserawasalsotestedforspecificityusingthefree-floatingPAPtechniqueonratcortex. Abbreviations: (GA)Glutaricanhydride (=)Non-reducedconjugate (PL)Polylysine (BSA)BovineSerumAlbumin |
| SpeciesReactivity |
|
| AntibodyType | PolyclonalAntibody |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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