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Millipore/MAB2081 | Anti-β-Catenin Antibody, clone 5H10/MAB2081/100 µg
Millipore/MAB2081 | Anti-β-Catenin Antibody, clone 5H10/MAB2081/100 µg
市场价：
¥5960.00
美元价：
3576.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB2081 |
| Replaces | 04-1002 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-β-CateninAntibody,clone5H10 |
| BackgroundInformation | Thetransmembrane,calciumdependentmoleculeE-cadherinisconsideredtobeoneofthekeymoleculesintheformationoftheintercellularjunctionalcomplexandestablishmentofpolarityinepithelialcells.Catenins,thealpha-subunit(102kDa),beta-subunit(88kDa)andgamma-subunit(82kDa),[reviewedinTakeichi,1991]areagroupofproteinsthatinteractwiththeintercellulardomainofE-cadherin,resultingincomplexesofE-cadherin/beta-catenin/alpha-cateninorE-cadherin/gamma-catenin/alpha-Catenin(Hincketal.,1994).Beta-catenin,thevertebratehomologueofDrosophilasegmentpolaritygenearmADIllo,andkeyelementoftheWntsignaltransductionpathways,isimportantinembryogenesis,celltransformation,andintercellularadhesionincludingtheadhesion,motilityandmetastasisofcancercells.Thus,cateninproteinexpression,regulationandlocalizationarepotentiallyimportantMarkerstopredictmotilityandinvasivenessofepithelialneoplasms.Theregionsofbothalpha-andbeta-catenin,locatedon5q21-22and3p21,havebeenshowntobeinvolvedinthedevelopmentofcertaintumors(seeJiang,1996;Schlosshaueretal.,2002)andreducedexpressionsofbothalpha-andbeta-cateninhavebeendescribedinvarioustumorsincludingbreastcarcinoma(Hashizumeetal,1996).Besidesadhesionfunctions,beta-cateninbindstothetumorsuppressorgeneAPC.APCmutationdisturbstheequilibriumandcytoplasmiclevelsoffreebeta-cateninlevelinthecellandmayhavearoleintumOrigenesis. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | ProteinAPurifiedIgGinliquidat1mg/mLin0.02MPBwith0.25MNaCl,pH7.6,containing0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfor1yearat2–8°Cfromdateofshipment.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Producedbyimmunizationwithrecombinanthumanbeta-catenin. |
| Clone | 5H10 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Recognizesanepitopeinthecarboxyterminus,aminoacids758-771,ofhumanbeta-catenin. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Beta-cateninisanadherensjunctionprotein.Adherensjunctions(AJs;alsocalledthezonulaadherens)arecriticalfortheestablishmentandmaintenanceofepitheliallayers,suchasthoseliningorgansurfaces.AJsmediateadhesionbetweencells,communicateasignalthatneighboringcellsarepresent,andanchortheactinCytoskeleton.Inservingtheseroles,AJsregulatenormalcellgrowthandbehavior.Atseveralstagesofembryogenesis,woundhealing,andtumorcellmetastasis,cellsformandleaveepithelia.Thisprocess,whichinvolvesthedisruptionandreestablishmentofepithelialcell-cellcontacts,mayberegulatedbythedisassemblyandassemblyofAJs.AJsmayalsofunctioninthetransmissionofthe"contactinhibition"signal,whichinstructscellstostopdividingonceanepithelialsheetiscomplete.[suppliedbyOMIM] |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P35222#InvolvedintheregulationofcelladhesionandinsignaltransductionthroughtheWntpathway. SIZE:781aminoacids;85497Da SUBUNIT:Twoseparatepoolsarefoundinthecytoplasm:oneisPSEN1/cadherin/catenincomplexwhichanchorstotheactincytoskeleton.TheotherpoolispartofalargecomplexcontainingAXIN1,AXIN2,APC,CSNK1A1andGSK3BthatpromotesphosphorylationonN-terminalSerandThrresiduesandubiquitinationofCTNNB1viaBTRCanditssubsequentdegradationbytheproteasome.Wnt-dependentactivationofDVLantagonizestheactionofGSK3B.WhenGSK3Bactivityisinhibitedthecomplexdissociates,CTNNB1isdephosphorylatedandisnolongertargetedfordestruction.ThestABIlizedproteintranslocatestothenucleus,whereitbindsTCF/LEF-1familymembers,TBP,BCL9andpossIBLyalsoRUVBL1andDUPLIN.BindsCTNNBIPandEP300.CTNNB1formsaternarycomplexwithLEF1andEP300thatisdisruptedbyCTNNBIP1binding(Bysimilarity).InteractswiththePDZdomainofTAX1BP3;thisinteractioninhibitsthetranscriptionalactivityofCTNNB1(Bysimilarity).InteractswithAJAP1,BAIAP1,CARM1,CTNNA3,CXADRandPCDH11Y.BindsSLC9A3R1.InteractswithGLIS2.InteractswithSLC30A9(Bysimilarity). SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Cytoplasmicwhenitisunstabilized(highlevelofphosphorylation)orboundtoCDH1.Translocatestothenucleuswhenitisstabilized(lowlevelofphosphorylation).InteractionwithGLIS2promotesnucleartranslocation. TISSUESPECIFICITY:Expressedinseveralhairfolliclecelltypes:basalandperipheralmatrixcells,andcellsoftheouterandinnerrootsheats.Expressedincolon. PTM:PhosphorylationbyGSK3BrequirespriorphosphorylationofSer-45byanotherkinase.PhosphorylationproceedsthenfromThr-41toSer-37andSer-33.&EGFstimulatestyrosinephosphorylation.PhosphorylationonTyr-654decreasesCDH1bindingandenhancesTBPbinding.&UbiquitinatedbyaE3ubiquitinligasecomplexcontainingUBE2D1,SIAH1,CACYBP/SIP,SKP1A,APCandTBL1X(Probable).Itsubiquitinationleadstoitssubsequentproteasomaldegradation. DISEASE:"SwissProt:P35222#ActivatingmutationsinCTNNB1haveoncogenicactivityresultingintumordevelopment.Somaticmutationsarefoundinvarioustumortypes,includingcoloncancers,ovarianandprostatecarcinomas,hepatoblastoma(HB),hepatocellularcarcinoma(HCC).HBsaremalignantembryonaltumorsmainlyaffectingyoungchildreninthefirstthreeyearsoflife.&DefectsinCTNNB1areacauseofpilomatrixoma(PTR)[MIM:132600];acommonbenignskintumor.&DefectsinCTNNB1areacauseofmedulloblastoma(MDB)[MIM:155255].MDBisamalignant,invasiveembryonaltumorofthecerebellumwithapreferentialmanifestationinchildren.&AchromosomalrearrangementinvolvingCTNNB1maybeacauseofsalivaryglandpleiomorphicadenomas(PA)[181030].Pleiomorphicadenomasarethemostcommonbenignepithelialtumorsofthesalivarygland.Translocationt(3;8)(p21;q12)withPLAG1." SIMILARITY:SwissProt:P35222##Belongstothebeta-cateninfamily.&Contains12ARMrepeats. |
| MolecularWeight | 94kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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