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当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/MAB1596 | Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9/MAB1596/100 µg
商品详细Millipore/MAB1596 | Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9/MAB1596/100 µg
Millipore/MAB1596 | Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9/MAB1596/100 µg
Millipore/MAB1596 | Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9/MAB1596/100 µg
商品编号: MAB1596
品牌: 密理博
市场价: ¥8020.00
美元价: 4812.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberMAB1596
Replaces04-1066
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-PostSynapticDensityProtein95Antibody,clone6G6-1C9
AlternateNames
  • PSD-95
BackgroundInformationPSD-95ispartofamultiproteincomplexrestrictedtothepost-synapticmembrane.ItisbelievedtoinitiateNMDAreceptorclusteringonthepostsynapticmembrane.ItsrestrictedexpressionmakesitanexcellentMarkerforpost-synapticmembraneregionsonneurons.
ProductInformation
FormatPurified
Control
  • Mousebrain,ratbraintissue.
PresentationPurifiedimmunoglobulin.LiquidinPBS,pH7.2with50%glyceroland0.09%sodiumazide.
StorageandShippingInformation
StorageConditionsStablefor6monthsat-20°Cinundilutedaliquotsfromdateofreceipt.Donotstorebelow-20°C.
HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIllityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
Applications
ApplicationAnti-PostSynapticDensityProtein95Antibody,clone6G6-1C9detectslevelofPostSynapticDensityProtein95&hasbeenpublished&validatedforuseinIC,IH&WB.
KeyApplications
  • Immunocytochemistry
  • Immunohistochemistry
  • WesternBlotting
ApplicationNotesImmunocytochemistry:
Representativeimagefromapreviouslot.
ConfocalfluorescentanalysisofPC12usingMAb1596MousemAB(Red).ActinfilamentshavebeenlabeledwithAlexaFluor488-Phalloidin(Green).NuclearisstainedwithDAPI(Blue).Membranestainingpositive.

Immunocytochemistry:
12.5µg/mL

WesternBlot:
1-5μg/mL(ECL)onratbrainlysate(20μg).

Optimalworkingdilutionsmustbedeterminedbytheenduser.
BIOLOGicalInformation
ImmunogenRecombinantratPSD-95.
Clone6G6-1C9
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostMouse
SpecificityReactswithaproteinof~100kDa,correspondingtotheapparentmolecularweightofPSD-95,onSDS-PAGEimmunoblotsofsamplesfromrat,mouseandbovine.Aslightlyhighermolecularweightbandisalsodetected.Inratbrainandmousebraintissueextractsabandof~75kDaisalsopresent.
IsotypeIgG2a
SpeciesReactivity
  • Bovine
  • Mouse
  • Rat
SpeciesReactivityNoteRat,mouseandbovine.Otherspecieshavenotbeentested.
AntibodyTypeMonoclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryThisgeneencodesamemberofthemembrane-associatedguanylatekinase(MAGUK)family.ItheteromultimerizeswithanotherMAGUKprotein,DLG2,andisrecruitedintoNMDAreceptorandpotassiumchannelclusters.ThesetwoMAGUKproteinsmayinteractatpostsynapticsitestoformamultimericscaffoldfortheclusteringofreceptors,ionchannels,andassociatedsignalingproteins.Multipletranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.[providedbyRefSeq]
GeneSymbol
  • PSD-95
PurificationMethodSeedatasheetforpurificationmethod.
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:P78352#InteractswiththecytoplasmictailofNMDAreceptorsubunitsandshaker-typepotassiumchannels.RequiredforsynapticplasticityassociatedwithNMDAreceptorsignaling.OverexpressionordepletionofDLG4changestheratioofexcitatorytoinhibitorysynapsesinhippocampalneurons.MayreducetheamplitudeofACCN3acid-evokedcurrentsbyretainingthechannelintracellularly.MayregulatetheintracellulartraffickingofADR1B(Bysimilarity).
SIZE:724aminoacids;80495Da
SUBUNIT:InteractsthroughitsfirsttwoPDZdomainswithGRIN2A,GRIN2B,GRIN2C,GRIN2D,ACCN3,certainspliceformsofGRIN1,KCND2,CXADRandSYNGAP1.InteractsthroughitssecondPDZdomainwiththePDZdomainofNOS1ortheC-terminusofCAPON.MayinteractwithHTR2A.Interactsthroughitsguanylatekinase-likedomainwithDLGAP1/GKAP,DLGAP2,DLGAP3,DLGAP4,MAP1A,andBEGAIN(Bysimilarity).InteractsthroughitsfirsttwoPDZdomainswithKCNA1,KCNA2,KCNA3,KCNA4andERBB4.InteractsthroughitsfirstPDZdomainwithGRIK2,KCNA4andCRIPT.InteractsthroughitsthirdPDZdomainwithNLGN1,andprobablywithNLGN2andNLGN3.Interactsthroughitsguanylatekinase-likedomainwithKIF13B.Isoform2interactsthroughanL27domainwithHGS/HRSandthefirstL27domainofCASK.
SUBCELLULARLOCATION:Cellmembrane;Peripheralmembraneprotein.Celljunction,synapse,postsynapticcellmembrane,postsynapticdensity.Celljunction,synapse.Note=Highlevelsinpostsynapticdensityofneuronsintheforebrain.AlsoinpresynapticregionofinhibitorysynapsesformedbycerebellarbasketcellsonaxonhillocksofPurkinjecells.
TISSUESPECIFICITY:Brain.
DOMAIN:SwissProt:P78352ThePDZdomain3mediatesinteractionwithADR1B.&TheL27domainneartheN-terminusofisoform2isrequiredforHGS/HRS-dependenttargetingtopost-synapticdensity.
PTM:Palmitoylationofisoform1isrequiredfortargetingtopostsynapticdensity(Bysimilarity).
SIMILARITY:BelongstotheMAGUKfamily.&Contains1guanylatekinase-likedomain.&Contains3PDZ(DHR)domains.&Contains1SH3domain.
MolecularWeight~100kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。