Millipore/05-915 |抗N-钙粘蛋白抗体，克隆13A9/05-915/100µ；L
市场价：
¥6780.00
美元价：
4068.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 05-915 |
| Replaces | 04-1126 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-N-CadherinAntibody,clone13A9 |
| AlternateNames |
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| BackgroundInformation | Cadherin-2(UniProtP19022;alsoknownasCD325,CDw325,N-cadherin,Neuralcadherin)isencodedbytheCDH2(alsoknownasCDHN,NCAD)gene(GeneID1000)inhuman.Cadherinsconstituteafamilyofcalcium-dependentcell-celladhesionproteinsthatplayimportantrolesintheembryonicdevelopmentandmaintenanceofnormaltissuearchitecture.Cadherinsarecomposedofanextracellulardomain(a.a.160-724ofhumanN-cadherin)withfivehomologousrepeatsthatmediatesadhesion,asinglepasstransmembranedomain(a.a.725-745ofhumanN-cadherin),andaconservedcytoplasmicdomain(a.a.746-906ofhumanN-cadherin)thatinteractswithcateninstolinkcadherinstotheactinCytoskeleton.Inaddition,aknownSrcsubstratep120ctnalsomodulatethestrengthofcadherin-dependentadhesionbyinteractingwithcadherinsattheirintracellularjuxtamembranedomain.Cadherinsaresynthesizedasprecursorproteinsthatmustbeproteolyticallycleavedtogeneratefunctional,matureproteins.NewlysynthesizedproN-cadherin(a.a.1-906)isphosphorylatedandproteolyticallyprocessedpriortotransporttotheplasmamembrane.Inaddition,Plakoglobin(gamma-catenin)andbeta-cateninassociateonlywithphosphorylatedproN-cadherin,whereasp120ctncanassociatewithbothphosphorylatedandnon-phosphorylatedproN-cadherin.TheN-terminalsignalandpropeptide(a.a.1-25and26-159ofofhumanN-cadherin)regionisproteolyticallyremovedandacoreN-cadherin-catenincomplexisassembledintheendoplasmicreticulumorGolgicompartmentpriortolocalizationattheplasmamembranewherelinkagetotheactincytoskeletoncanbeestablished. |
| ProductInformation | |
|---|---|
| Format | CultureSupernatant |
| Control |
|
| Presentation | Mousemonoclonalimmunoglobulinhybridomaculturesupernatantcontaining0.05%sodiumazidebeforetheadditionofglycerolto30%. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. |
| Applications | |
|---|---|
| Application | DetectN-cadherinusingthisAnti-N-CadherinAntibody,clone13A9validatedforuseinImmunocytochemistry,Immunohistochemistry,Immunoprecipitation,andWesternBlotting. |
| KeyApplications |
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| ApplicationNotes | ImmunohistochemistryAnalysis:Arepresentativelotimmunostainedtheextracellularmatrixofthestableplaquesandinthefibrouscapregionrichinvascularsmoothmusclecells(VSMCs)usingpatients-derivedparaffin-embeddedinternalcarotidarterytissuesections(Musumeci,G.,etal.(2014).Histol.Histopathol.29(6):707-719). ImmunohistochemistryAnalysis:ArepresentativelotdetectedstrongN-cadherinimmunoreactivityinparaffin-embeddedrectalcancer(RC)tissueswithpositiveregionallymphnodemetastasis(RLNM)status,whileonlyweakN-cadherinimmunoreactivitywasdetectedinRCwithnegativeRLNM,andnoN-cadherinstainingwasseeninnormalcolorectalepithelium(Fan,X.J.,etal.(2012).Br.J.Cancer.106(11):1735-1741). ImmunohistochemistryAnalysis:ArepresentativelotdetectedN-cadherinimmunoreactivityinformalin-fixed,paraffin-embeddedhepatocellularcarcinoma(HCC)tissuesections.AsignificantinversecorrelationwasfoundbetweenRUNX3andN-cadherinexpressionlevels(Tanaka,S.,etal.(2012).Int.J.Cancer.131(11):2537-2546). WesternBlottingAnalysis:ArepresentativelotdetectedanupregulatedN-cadherinexpressioninCCL185carcinomacellsfollowingtransientEpstein-Barrvirus(EBV)infection.TheEMT-likephenotyperemainedevenaftervirallossbycultureselectionpressurewithdrawal(Queen,K.J.,etal.(2013).Int.J.Cancer.132(9):2076-2086). WesternBlottingAnalysis:ArepresentativelotdetectedN-cadherininHep3B,Huh7,HLFandSK-Hep1humanhepatocellularcarcinoma(HCC)celllysates(Tanaka,S.,etal.(2012).Int.J.Cancer.131(11):2537-2546). WesternBlottingAnalysis:Arepresentativelotdetectedboththeunprocessed(pro-)andprocessed(mature)formsofN-cadherininHeLacelllysate(Wahl,J.K.3rd.,etal.(2003).J.Biol.Chem.278(19):17269-17276). WesternBlottingAnalysis:ArepresentativelotdetectedN-cadherininWI-38humanfibroblastlysate,butnotinJArhumanplacentalchoriocarcinomacelllysate(Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77). ImmunocytochemistryAnalysis:ArepresentativelotdetectedN-cadherinimmunoreactivitylocalizedprimarilyatthecell-cellbordersbyfluorescentimmunocytochemistrystainingof1%paraformaldehyde-fixed,methanol-permeABIlizedHeLacells(Wahl,J.K.3rd.,etal.(2003).J.Biol.Chem.278(19):17269-17276). ImmunocytochemistryAnalysis:ArepresentativelotdetectedN-cadherinimmunoreactivitycolocalizedwiththoseofalpha-andbeta-cateninbydualfluorescentimmunocytochemistrystainingoffixedWI-38humanfibroblasts(Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77). ImmunoprecipitationAnalysis:Representativelotsco-immunoprecipitatedalpha-catenin,beta-catenin,andplakoglobinwithN-cadherinfromWI-38humanfibroblastandHeLacelllysates(Wahl,J.K.3rd.,etal.(2003).J.Biol.Chem.278(19):17269-17276;Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77). |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | BacteriallyexpressedhumanN-cadherincytoplasmicdomainMBPfusionprotein(Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77). |
| Epitope | Cytoplasmicdomain. |
| Clone | 13A9 |
| Host | Mouse |
| Specificity | Clone13A9recognizesN-cadherin,butnotP-,E-,orM-cadherin(Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77). |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| PurificationMethod | Unpurified |
| UniProtNumber | |
| MolecularWeight | ~140kDaobserved.Targetbandsizeappearslargerthanthecalculatedmolecularweightsof82.03kDa(mature)and99.81/97.04kDa(isoform1/2pro-form)duetoposttranslationalglycosylationandphosphorylation. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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