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Millipore/04-1103 | Anti-E-cadherin Antibody, clone EP700Y, rabbit monoclonal/04-1103/100 µL
Millipore/04-1103 | Anti-E-cadherin Antibody, clone EP700Y, rabbit monoclonal/04-1103/100 µL
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 04-1103 |
| Description | Anti-E-cadherinAntibody,cloneEP700Y,rabbitmonoclonal |
| AlternateNames |
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| BackgroundInformation | CadherinsareafamilyoftransmembraneglycoproteinsinvolvedinCa2+-dependentcelltocellinteractions.Themembersofthefamilyaredifferentiallyexpressedinvarioustissuesandfunctioninthemaintenanceoftissueintegrity,morphogenesis,andmigration.CadherinsaredividedintotypeIandtypeIIsubgroups.TypeIcadherinsincludeepithelialcadherin(E-cadherin),neuralcadherin(N-cadherin),placentalcadherin(P-cadherin)andretinalcadherin(R-cadherin).TypeIIincludekidneycadherin(K-cadherin)andosteoblastcadherin(OB-cadherin).OneofthebestcharacterizedcadherinsisE-cadherin(epithelialcadherinorcadherin-1).ItconsistsofcalciumbindingextracellulardomainsresponsIBLeforinteractionswithotherE-cadherinmoleculesonneighboringcellsandinteractionwithintegrinalphaEbeta7.ThecytoplasmicdomainofE-cadherinislinkedtotheactinCytoskeletonthroughcytoplasmiccateninproteins,thusestablishingacomplexlocalizedtoadherensjunctions.IncarcinomasE-cadherinisfrequentlydownregulated,whichisconsistentwithitsfunctionofaninvasionsuppressorinnormalepithelia. |
| ProductInformation | |
|---|---|
| Format | Unpurified |
| Control |
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| Presentation | RabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20ºCfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Syntheticpeptidecorrespondingtoresiduesinthe5thcadherindomainofhumanE-cadherin. |
| Clone | EP700Y |
| Host | Rabbit |
| Specificity | ThisantibodyrecognizesE-cadherin. |
| Isotype | IgG |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| PurificationMethod | Unpurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:Cadherinsarecalcium-dependentcelladhesionproteins.Theypreferentiallyinteractwiththemselvesinahomophilicmannerinconnectingcells;cadherinsmaythuscontributetothesortingofheterogeneouscelltypes.CDH1isinvolvedinmechanismsregulatingcell-celladhesions,mobilityandproliferationofepithelialcells.Hasapotentinvasivesuppressorrole.Itisaligandforintegrinalpha-E/beta-7. E-Cad/CTF2promotesnon-amyloidogenicdegradationofAbetaprecursors.HasastronginhibitoryeffectonAPPC99andC83production. SUBUNITSTRUCTURE:Homodimer;disulfide-linked.Interactsdirectly,viathecytoplasmicdomain,withCTNNB1orJUPtoformthePSEN1/cadherin/cateninadhesioncomplexwhichconnectstotheactinskeletonthroughtheactinbindingofalpha-catenin.InteractionwithPSEN1,cleavesCDH1resultinginthedisassociationofcadherin-basedadherensjunctions(CAJs).InteractswithAJAP1,CTNND1andDLGAP5. SUBCELLULARLOCATION:Celljunction.Cellmembrane;Single-passtypeImembraneprotein.Note:ColocalizeswithDLGAP5atsitesofcell-cellcontactinintestinalepithelialcells.AnchoredtoactinMICROFILamentsthroughassociationwithalpha-,beta-andgamma-catenin.Sequentialproteolysisinducedbyapoptosisorcalciuminflux,resultsintranslocationfromsitesofcell-cellcontacttothecytoplasm. TISSUESPECIFICTIY:Non-neuralepithelialtissues. PTM:Duringapoptosisorwithcalciuminflux,cleavedbyamembrane-boundmetalloproteinase(ADAM10),PS1/gamma-secretaseandcaspase-3toproducefragmentsofabout38kDa(E-CAD/CTF1),33kDa(E-CAD/CTF2)and29kDa(E-CAD/CTF3),respectively.Processingbythemetalloproteinase,inducedbycalciuminflux,causesdisruptionofcell-celladhesionandthesubsequentreleaseofbeta-cateninintothecytoplasm.Theresidualmembrane-tetheredcleavageproductisrapidlydegradedviaanintracellularproteolyticpathway.Cleavagebycaspase-3releasesthecytoplasmictailresultingindisintegrationoftheactinmicrofilamentsystem.Thegamma-secretase-mediatedcleavagepromotesdisaaaemblyofadherensjunctions. INVOLVEMENTINDISEASE:DefectsinCDH1areinvolvedindysfunctionofthecell-celladhesionsystem,triggeringcancerinvasion(gastric,breast,ovary,endometriumandthyroid)andmetastasis. DefectsinCDH1areacauseofgastriccancer[MIM:137215];alsoknownashereditaryfamilialdiffusegastriccancer(HDGC). DefectsinCDH1areacauseofsusceptibilitytoendometrialcancer[MIM:608089]. DefectsinCDH1areassociatedwithovariancancer[MIM:167000].OvariancanceristheleADIngcauseofdeathfromgynecologicmalignancy.Itischaracterizedbyadvancedpresentationwithloco-regionaldisseminationintheperitonealcavityandtherareincidenceofvisceralmetastases.Thesetypicalfeaturesrelatetothebiologyofthedisease,whichisaprincipaldeterminantofoutcome. SEQUENCESIMILARITIES:Contains5cadherindomains. SEQUENCECAUTION:ThesequenceAAA61259.1differsfromthatshown.Reason:Frameshiftatpositions16,22,25,28,31,34,52,67,73,76,94,102,633and636. |
| MolecularWeight | ~135/80kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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