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Millipore/MAB3420 | Anti-Tau-1 Antibody, clone PC1C6/MAB3420/100 µg
Millipore/MAB3420 | Anti-Tau-1 Antibody, clone PC1C6/MAB3420/100 µg
市场价：
¥7800.00
美元价：
4680.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB3420 |
| Replaces | AB1512 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-Tau-1Antibody,clonePC1C6 |
| BackgroundInformation | Tau,amicrotubulebindingproteinwhichservestostABIlizemicrotubulesingrowingaxons,isfoundtobehyperphosphorylatedinpairedhelicalfilaments(PHF),themajorfibrouscomponentofneurofibrillarylesionsassociatedwithAlzheimer’sdisease.HyperphosphorylationofTauisthoughttobethecriticaleventleADIngtotheassemblyofPHF.SixTauproteinisoformshavebeenidentified,allofwhicharephosphorylatedbyglycogensynthasekinase3(GSK3).Cellularandsubcellularlocalization:Insitu,anti-tau-1hasastringentspecificityfortheaxonsofneurons.Theantibodydoesnotstainthecellbodiesordendritesofneurons,nordoesitstainanyothercelltype(4).However,thisinvivointracellularspecificityisnotmaintainedinculture:anti-tau-1stainstheaxon,cellbodies,anddendritesofrathippocampalneuronsgrowninculture(5).Thespecificityofanti-tau-1wasoriginallythoughttorepresenttherestrictedexpressionoftautoaxons.Laterstudiesrevealedthatthisspecificityisdependantonthestateofphosphorylation.Indephosphorylatedsamples(samplestreatedwithalkalinephosphatase)anti-tau-1stainsastrocytes,perineuronalglialcells,andtheaxons,cellbodiesanddendritesofneurons,whileinuntreatedsamples,anti-tau-1stainsonlyaxons(6).(Theepitoperecognizedbyanti-tau-1isprobablyatornearaphosphorylatedsite.) |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | 0.02Mphosphatebuffer,pH7.6,0.25MNaCl,and0.1%sodiumazide |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. |
| Applications | |
|---|---|
| Application | Anti-Tau-1Antibody,clonePC1C6isanantibodyagainstTau-1foruseinIH&WBwithmorethan65productcitations. |
| KeyApplications |
|
| ApplicationNotes | Westernblot:Bovinebrainmicrotubuleproteinspurifiedbytwocyclesofassemblyanddisassembly(9)aredissolvedinSDS-PAGEsamplebuffer.Fivemicrogramsofthemicrotublepreparationperlaneisloadedontoa4%to20%SDS-PAGEgradientgelalongsidemolecularweightMarkers(14.3-200kD).Afterseparationbyelectrophoresis,theproteinsareblottedontonitrocellulose.Tauisdetectedasaseriesof5bands(52-68kD)withapproximately5ng/mLofanti-tau-1. Immunohistochemistry:5μg/mL;stainsaxonsintissueprimarily,howeverincultureTauexpressionisnotrestrictedtojustaxons. Optimalworkingdilutionsmustbedeterminedbyenduser. ImmunohistochemistryProtocol Dephosphorylationoftissuesections(optional) DephosphorylationwithalkalinephosphataseisrecommendedforstainingneurofibrillarytanglesinAlzheimer"sbraintissuewithanti-tau-1(6).Thistreatmentchangesthestainingpatternofanti-tau-1toincludecellbodies,dendritesandaxonsofneurons.Inuntreatedsamples,anti-tau-1stainsaxonsonly. 1.Incubatetissuesectionsat+32°Cfor2.5hourswithconstantagitationinthefollowingsolution:100mMTris-HCl,pH8.0;130units/mLalkalinephosphatase,1mMPMSF,10μg/mLpepstatinand10μg/mLleupeptin. 2.Rinsesectionstwice,3minperrinse,with100mMTris-HCl,pH8.0. Anti-tau-1staining 1.Blocknon-specificbindingbyincubatingsectionsinPBScontaining1%(v/v)normalanimalserum,and0.03%(w/v)TritonX-100.Theanimalserumshouldbefromthesamespeciesasthesecondaryantibody. 2.Rinse3timeswithPBS,3minperrinse. 3.Incubatesectionswithanti-tau-1,approximately5μg/mL,dilutedinPBScontaining1%(v/v)normalanimalserum. 4.WashwithPBS,changingthesolution3timesovera3minperiod. 5.Detectwithastandardsecondaryantibodydetectionsystem(10-13). |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Purifieddenaturedbovinemicrotubuleassociatedproteins. |
| Clone | PC1C6 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Bindstoallknownelectrophoreticspeciesoftauinhuman,ratandbovinebrain(one-dimensionalSDS-PAGE).HoweverthereissomeunphosphorylatedbiaswithclonePC1C6asitseemtorecognizeonlydephosphorylatedserinesitesat195,198,199,and202{Szendrei,etal1993;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi-cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7680727}.AlsoseeBillingsley&Kincaid,1997BiochemJ323:577-591foradditionalmappinginformationonPC1C6. |
| Isotype | IgG2a |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesthemicrotubule-associatedproteintau(MAPT)whosetranscriptundergoescomplex,regulatedalternativesplicing,givingrisetoseveralmRNAspecies.MAPTtranscriptsaredifferentiallyexpressedinthenervoussystem,dependingonstageofneuronalmaturationandneurontype.MAPTgenemutationshavebeenassociatedwithseveralneurodegenerativedisorderssuchasAlzheimer"sdisease,Pick"sdisease,frontotemporaldementia,cortico-basaldegenerationandprogressivesupranuclearpalsy. |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P10636#Promotesmicrotubuleassemblyandstability,andmightbeinvolvedintheestablishmentandmaintenanceofneuronalpolarity.TheC-terminusbindsaxonalmicrotubuleswhiletheN-terminusbindsneuralplasmamembranecomponents,suggestingthattaufunctionsasalinkerproteinbetweenboth.Axonalpolarityispredeterminedbytaulocalization(intheneuronalcell)inthedomainofthecellbodydefinedbythecentrosome.TheshortisoformsallowplasticityoftheCytoskeletonwhereasthelongerisoformsmaypreferentiallyplayaroleinitsstabilization. SIZE:758aminoacids;78878Da SUBUNIT:InteractswithPSMC2throughSQSTM1(Bysimilarity).InteractswithSQSTM1whenpolyubiquitinated. SUBCELLULARLOCATION:Cytoplasm,cytosol.Cellmembrane.Note=Mostlyfoundintheaxonsofneurons,inthecytosolandinassociationwithplasmamembranecomponents. TISSUESPECIFICITY:Expressedinneurons.IsoformPNS-tauisexpressedintheperipheralnervoussystemwhiletheothersareexpressedinthecentralnervoussystem.DEVELOPMENTALSTAGE:Four-repeat(typeII)tauisexpressedinanadult-specificmannerandisnotfoundinfetalbrain,whereasthree-repeat(typeI)tauisfoundinbothadultandfetalbrain. DOMAIN:SwissProt:P10636Thetau/MAPrepeatbindstotubulin.TypeIisoformscontain3repeatswhiletypeIIisoformscontain4repeats. PTM:PhosphorylationatserineandthreonineresiduesinS-PorT-Pmotifsbyproline-directedproteinkinases(PDPK:CDC2,CDK5,GSK-3,MAPK)(only2-3sitesperproteinininterphase,seven-foldincreaseinmitosis,andinPHF-tau),andatserineresiduesinK-X-G-SmotifsbyMAP/microtubuleaffinity-regulatingkinase(MARK)inAlzheimerdiseasedbrains.Phosphorylationdecreaseswithage.Phosphorylationwithintau"srepeatdomainorinflankingregionsseemstoreducetau"sinteractionwith,respectively,microtubulesorplasmamembranecomponents.PhosphorylationonSer-610,Ser-622,Ser-641andSer-673inseveralisoformsduringmitosis.&Polyubiquitinated.RequiresfunctionalTRAF6andmayprovokeSQSTM1-dependentdegradationbytheproteasome(Bysimilarity).PHF-taucanbemodifiedbythreedifferentformsofpolyubiquitination."Lys-48"-linkedpolyubiquitinationisthemajorform,"Lys-6"-linkedand"Lys-11"-linkedpolyubiquitinationalsooccur.&GlycationofPHF-tau,butnotnormalbraintau.Glycationisanon-enzymaticpost-translationalmodificationthatinvolvesacovalentlinkagebetweenasugarandanaminogroupofaproteinmoleculeformingketoamine.Subsequentoxidation,fragmentationand/orcross-linkingofketoamineleadstotheproductionofadvancedglycationendproducts(AGES).GlycationmayplayaroleinstabilizingPHFaggregationleadingtotangleformationinAD. DISEASE:SwissProt:P10636#InAlzheimerdisease,theneuronalcytoskeletoninthebrainisprogressivelydisruptedandreplacedbytanglesofpairedhelicalfilaments(PHF)andstraightfilaments,mainlycomposedofhyperphosphorylatedformsofTAU(PHF-TAUorADP-TAU).&DefectsinMAPTareacauseoffrontotemporaldementiaandparkinsonismlinkedtochromosome17(FTDP17)[MIM:600274,172700];alsocalledfrontotemporaldementia(FTD)orhistoricallytermedPickcomplex.Thisformoffrontotemporaldementiaischaracterizedbypreseniledementiawithbehavioralchanges,deteriorationofcognitivecapacitiesandlossofmemory.Insomecases,parkinsoniansymptomsareprominent.Neuropathologicalchangesincludefrontotemporalatrophyoftenassociatedwithatrophyofthebasalganglia,substantianigra,amygdala.Inmostcases,proteintaudepositsarefoundinglialcellsand/orneurons.&DefectsinMAPTareacauseofpallido-ponto-nigraldegeneration(PPND)[MIM:168610].Theclinicalfeaturesincludeocularmotilityabnormalities,dystoniaandurinaryincontinence,besidesprogressiveparkinsonismanddementia.&DefectsinMAPTareacauseofcorticobasaldegeneration(CBD).Itismarkedbyextrapyramidalsignsandapraxiaandcanbeassociatedwithmemoryloss.NeuropathologicfeaturesmayoverlapAlzheimerdisease,progressivesupranuclearpalsy,andParkinsondisease.&DefectsinMAPTareacauseofprogressivesupranuclearpalsy(PSP)[MIM:601104,260540];alsoknownasSteele-Richardson-Olszewskisyndrome.PSPischaracterizedbyakinetic-rigidsyndrome,supranucleargazepalsy,pyramidaltractdysfunction,pseudobulbarsignsandcognitivecapacitiesdeterioration.Neurofibrillarytanglesandgliosisbutnoamyloidplaquesarefoundindiseasedbrains.Mostcasesappeartobesporadic,withasignificantassociationwithacommonhaplotypeincludingtheMAPTgeneandtheflankingregions.Familialcasesshowanautosomaldominantpatternoftransmissionwithincompletepenetrance;geneticanalysisofafewcasesshowedtheoccurrenceoftaumutations,includingadeletionofAsn-613.&DefectsinMAPTmaybeacauseofhereditarydysphasicdisinhibitiondementia(HDDD)[MIM:607485].HDDDisafrontotemporaldementiacharacterizedbyprogressivecognitivedeficitswithmemorylossandpersonalitychanges,severedysphasicdisturbancesleadingtomutism,andhyperphagia. SIMILARITY:Contains4Tau/MAPrepeats. |
| MolecularWeight | 5bands(52–68kDa) |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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