密理博/NS225 |神经突起生长分析试剂盒（1µm）/NS225/12分析
市场价：
¥5940.00
美元价：
3564.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | NS225 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | NeuriteOutgrowthAssayKit(1µm) |
| BackgroundInformation | ApplicationCHEMICON"sNS225NeuriteOutgrowthAssayKit(1μm)utilizesmicroporoustissuecultureinserttechnologyfromMilliporeandisbasedontheuseofMillicellcellcultureinserts(chambers)containingapermeablemembranewith1μmporesatthebase.Theinsertsaredesignedtofitintoareceivervessel,whichwheninusecontainsdifferentiationmediaincontactwiththebottomoftheinsertmembrane.Thepermeablemembranesallowfordiscriminationbetweenneuritesandcellbodies,asprojectingneuriteswillpasseasilythroughtheporesbutcellbodieswillnot.Therefore,byinducingneuritestotraversethesemembranepores,apurifiedpopulationofneuritesislocatedontheundersideofthemembrane,distaltothesurfaceonwhichneuralcellbodiesaredeposited.ThiskithashasbeenspecificallydesignedforandvalidatedwithPC12cells.ThediameterofPC12cellbodiesisrelativelysmallcomparedtothatofmanyothercelltypescommonlyusedinneuriteoutgrowthassays[5,6,7].Asaresult,manyPC12cellbodiesmaypassthoughporeslargerthan1μm,significantlyobscuringneuriteoutgrowthassayresults.Usinga1μmporesizegreatlyreducesbackgroundandimprovesthesignal-to-noiseratioinstudiesusingPC12cells.Useofthis1μmporesizewithlargercelltypesthatextendwiderdiameterneurites,e.g.,N1E-115cells,willgreatlydiminishthenumberofneuritesabletoprojectthroughtotheundersideofthemembrane,andisthusnotrecommended.Theassayisasimple,efficient,andversatilesystemforthequantitativedeterminationofcompoundsthatinfluenceneuriteformationandrepulsion.Withthissystem,itispossIBLetoscreennumerousBIOLOGicalandpharmacologicalagentssimultaneously,directlyevaluateadhesionandguidancereceptorfunctionsresponsibleforneuriteextensionandrepulsion,aswellasanalyzegenefunctionintransfectedcells.Themicroporousfilterallowsforbiochemicalseparationandpurificationofneuritesandcellbodiesfordetailedmolecularanalysisofproteinexpression,signaltransductionprocessesandidentificationofdrugtargetsthatregulateneuriteoutgrowthorretractionprocesses.ReagentsandmaterialssuppliedintheNS225NeuriteOutgrowthAssayKitaresufficientfor12tests. |
| MaterialsRequiredbutNotDelivered | 1.Desiredextracellularmatrixmembranecoatingprotein,e.g.,bovinecollagenIormouselaminin. 2.Tissuecultureinstruments/supplies(including37°Cincubator,growthmedia,flasks/plates,detachmentbuffer,etc.)forcelltypeofinterest. 3.Differentiationmedia/factorsforcelltypeofinterest(e.g.,nervegrowthfactor,serum-freemedia). 4.1Xphosphatebufferedsaline(PBS)withCa2+/Mg2+. 5.Invertedmicroscopeforneuritevisualizationonmembraneinserts. 6.Parafilmandspectrophotometerforneuritestainextractionandquantification. 7.Proteinlysisbuffer(optional) |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Whenstoredat2-8°C,thekitcomponentsarestableuptotheexpirationdate.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate. |
| Applications | |
|---|---|
| Application | TheNS225NeuriteOutgrowthAssayKit(1μm)isbasedontheuseofMillicellcellcultureinserts(chambers)containingapermeablemembranewith1μmporesatthebase. |
| BiologicalInformation | |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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