Millipore/MAB5386 |抗神经生长因子受体（p75）抗体，克隆ME20.4/MAB5386/100µ；G
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB5386 |
| Replaces | 04-1111 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-NerveGrowthFactorReceptor(p75)Antibody,cloneME20.4 |
| AlternateNames |
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| BackgroundInformation | ThelowaffinityNGFR(Nervegrowthfactorreceptor)isa75kDamembrane-spanningglycoproteinlackingintrinsictyrosinekinaseactivity.p75NGFRinteractswithTrkA,thehighaffinityNGFreceptorandpotentiatesTrkAsignalingatlowNGFconcentrations.Thep75receptorbindsnervegrowthfactor,brain-derivedneurotrophicfactor,neurotrophin-3andneurotrophin-4withvaryingspecificities.Thep75NGFRplaysanimportantroleinneurotrophicfactorsignalingandhasbeenshowntomodulatethesusceptibilityofselectivecellularpopulationstoprogrammedcelldeath.ItisexpressedonmanyneuronalcellstypesincludingmanyembryonicformsandthereceptorcanbeusedtoisolateneuronalProgenitorcells |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | ProteinAPurifiedmouseimmunoglobulinin20mMsodiumphosphate,250mMNaCl,pH.7.6,withnopreservatives. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfor1yearat2–8°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. |
| Applications | |
|---|---|
| Application | Anti-NerveGrowthFactorReceptor(p75)Antibody,cloneME20.4detectslevelofNerveGrowthFactorReceptor(p75)&hasbeenpublished&validatedforuseinFC,IC,IH,IH(P),IP,RIA&WB. |
| KeyApplications |
|
| ApplicationNotes | Westernblot:RIPAlysatesofhumanHS294Tcells;1μg/mL;recognizes~69-75kDabanddependingupongelconditions. Immunohistochemistry:5-10μg/mLonfrozentissue. Immunohistochemistryonformalin-fixed,paraffinembeddedsections. Immunocytochemistry:5-10μg/mL EMImmunohistochemistry{Caneva,etal1995}.http://www.scripps.edu/bcmd/pdfarea/issue_09/soligo.pdf ImmunoabsorptivepurificationofNGF-receptors. InhibitionofNGFbindingtop-75NGFR Immunoprecipitationofp75 FACS:unfixedcells RIA Optimalworkingdilutionsmustbedeterminedbytheenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Humanmelanomacellline(Ross,1984). |
| Epitope | withina.a.1-160,extracellulardomainofhp75. |
| Clone | ME20.4 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Reactswiththelowaffinitynervegrowthfactorreceptor(NGFRp75).Theepitoperecognizedbytheantibodymapswithinthethirdorfourthcysteine-richrepeatsoftheNGFRmolecule.Reactivityhasnotbeenobservedwithrat,chicken,ormouseNGFR. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Nervegrowthfactorreceptorcontainsanextracellulardomaincontainingfour40-aminoacidrepeatswith6cysteineresiduesatconservedpositionsfollowedbyaserine/threonine-richregion,asingletransmembranedomain,anda155-aminoacidcytoplasmicdomain.Thecysteine-richregioncontainsthenervegrowthfactorbindingdomain. |
| GeneSymbol |
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| Non-ReactiveSpecies |
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| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P08138#LowaffinityreceptorwhichcanbindtoNGF,BDNF,NT-3,andNT-4.Canmediatecellsurvivalaswellascelldeathofneuralcells. SIZE:427aminoacids;45183Da SUBUNIT:Homodimer;disulfide-linked.Interactswithp75NTR-associatedcelldeathexecutor.InteractswithTRAF2,TRAF4,TRAF6,PTPN13andRANBP9.InteractsthroughTRAF6withSQSTM1whichbridgesNGFRtoNTRK1.InteractswithBEX1andNGFRAP1/BEX3. SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. DOMAIN:SwissProt:P08138DeathdomainisresponsIBLeforinteractionwithRANBP9. PTM:N-andO-glycosylated.&O-linkedglycansconsistofGal(1-3)GalNAccoreelongatedby1or2NeuNAc.&Phosphorylatedonserineresidues. SIMILARITY:Contains1deathdomain.&Contains4TNFR-Cysrepeats. |
| MolecularWeight | ~69-75kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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