Millipore/MAB5326 |抗巢蛋白抗体，克隆10C2/MAB5326/100µ；G
市场价：
¥6780.00
美元价：
4068.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB5326 |
| Replaces | AB5922 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-NestinAntibody,clone10C2 |
| BackgroundInformation | Nestin,alargeintermediatefilamentprotein(classTypeVI)expressedduringdevelopmentandinmyotendinousandneuromuscularjunctions.Nestinexpressionisrestricted,typicallydisappearingbyE18.NestinisthoughttobeareasonableneuronalMarker;however,recentstudieshavefoundnestinexpressioninothercelltypessuchasendothelialcells{FoliaBIOLOGica,44(5):155,1998}.Nestinidentifiesthemostprimitiveneuroepitheliumbutalsoidentifiesmanyotherembryonictissues,soitisnotspecificforCNS.NestinexpressionisseeninalmostallGBMs(Glioblastomamulitformes)andmanymelanomas(bothprimaryandmetastatic)butnotinanymetastaticcarcinoma. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | ProteinAPurifiedmouseimmunoglobulinin20mMsodiumphosphate,250mMNaCl,pH.7.6,with0.1%sodiumazideasapreservative. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Antibodiesmaybestoredat4°Cor-20°C.Avoidrepeatedfreeze/thawcycles. Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200μLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer"scap. |
| Applications | |
|---|---|
| Application | ThisAnti-NestinAntibody,clone10C2isvalidatedforuseinIC,IH,IH(P),WBforthedetectionofNestin. |
| KeyApplications |
|
| ApplicationNotes | Immunoblotting:1:1,000-1:2,000ondenaturedprotein.Westernblot:1:1000-1:5000.Recognizesa220-240kDAbandondenaturingSDSgels.Thereisalsoa60-64kDabandpresentinmostlysates,theidentityofthissmallerfragmentisunknownbutitisnotseeninlysatesnotcontaininghumannestinprotein. WI-38(ATCC#CCL75)canbeusedasapositivecontrol.U251celllineisalsopositive. Immunocytochemistry:1:200oncellsfixedin4%paraformaldehydeandpermeABIlizedwith0.02%Triton Immunohistochemistry:paraformaldehydeisthepreferredfixative:1:200onformalinfixed,paraffinembeddedtissuesections.Usetriton0.02%inblockonly;dilutionbuffer0.1%BSA;incubations1-2hoursRT.Humanfetalbrainissuggestedasapositivecontrol.(CAMessametal,Develop.BrainRes,134:87-92,2002;Gu,etal.,BrainRes.943:174-180,2002).Filamentousstaininginthecytoplasmofcells Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BiologicalInformation | |
|---|---|
| Immunogen | Fusionprotein. |
| Epitope | human |
| Clone | 10C2 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | ReactswithhumanNestin,withweakreactivitytomonkeynestin.Notreactivewithrodentnestin. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | NestinisanintermediatefilamentproteinthatwasfirstidentifiedwithamonoclonalantibodybyHockfieldandMcKay(1985)[PubMed4078630].Itisexpressedpredominantlyinstemcellsofthecentralnervoussystemintheneuraltube.Uponterminalneuraldifferentiation,nestinisdownregulatedandreplacedbyneurofilaments.[suppliedbyOMIM] |
| GeneSymbol |
|
| Non-ReactiveSpecies |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P48681#MayplayaroleinthetraffickinganddistributionofIFproteinsandpotentiallyothercellularfactorstodaughtercellsduringProgenitorcelldivision(Bysimilarity). SIZE:1621aminoacids;177439Da SUBUNIT:InteractswithFHOD3(Bysimilarity). TISSUESPECIFICITY:CNSstemcells. DEVELOPMENTALSTAGE:Uponterminalneuraldifferentiation,nestinisdown-regulatedandreplacedbyneurofilaments. SIMILARITY:SwissProt:P48681##Belongstotheintermediatefilamentfamily. |
| StemCellType |
|
| MolecularWeight | 200-220kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
