Millipore/MAB3418 |抗MAP2抗体，克隆AP20/MAB3418/200µ；G
市场价：
¥8020.00
美元价：
4812.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB3418 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Anti-MAP2Antibody,cloneAP20 |
| AlternateNames |
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| BackgroundInformation | MAP-2(microtubuleassociatedprotein-2)isoneofseveralhighmolecularweightproteinsthatplayanimportantroleinbrainneuronmicrotubuleassembly.Inadditiontoitsassociationwithmicrotubules,MAP-2associateswithneurofilamentsandactinfilamentssuggestingthatitmayguideinteractionamongmicrotubules,othercytoskeletalelements,andcytoplasmicorganelles.MAP-2isastringentMarkerforneurons.Inaddition,MAP-2displaysintracellularspecificity.Inthecentralnervoussystem,MAP-2isconfinedtoneuronalcellbodiesanddendrites.Thereareexceptions,however,wheresomeaxonsstainpositiveforsmallamountsofMAP-2.MAP-2isuniformlydistributedthroughoutthecellwhenfirstexpressedinculturedneuronsbutbecomesselectivelylocalizedasdendriticdevelopmentproceeds.(Caceres,1986;Dotti,1987). |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | PurifiedmousemonoclonalIgG1inbuffercontaining0.02MPhosphatebuffer,pH7.6,0.25MNaClwith0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor6monthsat2-8°Cinundilutedaliquotsfromdateofreceipt. Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200µLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer’scap. |
| Applications | |
|---|---|
| Application | Anti-MAP2Antibody,cloneAP20detectslevelofMAP2&hasbeenpublished&validatedforuseinIH&WB. |
| KeyApplications |
|
| ApplicationNotes | WesternBlot: 60ng/mL.InSDS-PAGE,MAP-2fromadultratmigratesasacloselyassociateddoublethavingamolecularweightofapproximately280-300kDrepresentingtheMAP-2Aand-2Bisoformsbutnotthe70kDaMAP-2Cisoform.However,earlyinbraindevelopment(postnatalday10inrats),MAP-2migratesasasinglebandthatisidenticaltothelowermolecularweightbandoftheadultMAP-2doublet(MAP-2b).Laterindevelopment(postnataldays17-18),themobilityofMAP-2changestotheadultdoubletform.(Inthespinalcord,conversiontotheadultformoccursearlier).Usersshouldrun4%-20%SDS-PAGEgrADIentgels. Immunohistochemistry: 5µg/mL(4%PFAfixedfrozensections). Immunohistochemistry: 5µg/mLofapreviouslotwasused.Anti-MAP-2canbeusedtostaintissue(brainorspinalcord)fixedwithparaformaldehyde. Optimalworkingconcentrationsmustbedeterminedbytheenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Bovinebrainmicrotubuleprotein. |
| Clone | AP20 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | MAB3418bindsspecificallytoMAP-2. |
| Isotype | IgG |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Ratandmouse.Expectedtocross-reactwithhuman,cow,chicken,quail,andXenopus. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinthatbelongstothemicrotubule-associatedproteinfamily.Theproteinsofthisfamilyarethoughttobeinvolvedinmicrotubuleassembly,whichisanessentialstepinneurogenesis.Theproductsofsimilargenesinratandmouseareneuron-specificcytoskeletalproteinsthatareenrichedindentrites,implicatingaroleindeterminingandstABIlizingdentriticshapeduringneurondevelopment.Anumberofalternativelysplicedvariantsencodingdistinctisformshavebeendescribed. |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P11137#TheexactfunctionofMAP2isunknownbutMAPsmaystabilizethemicrotubulesagainstdepolymerization.Theyalsoseemtohaveastiffeningeffectonmicrotubules. SIZE:1827aminoacids;199539Da PTM:MAP2A/cisphosphorylated.PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:SwissProt:P11137##Contains3Tau/MAPrepeats. |
| MolecularWeight | 230kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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