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当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/MAB1637 | Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)/MAB1637/100 µL
商品详细Millipore/MAB1637 | Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)/MAB1637/100 µL
Millipore/MAB1637 | Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)/MAB1637/100 µL
Millipore/MAB1637 | Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)/MAB1637/100 µL
商品编号: MAB1637
品牌: 密理博
市场价: ¥7180.00
美元价: 4308.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberMAB1637
Replaces04-1049
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-TubulinAntibody,betaIIIisoform,CT,cloneTU-20(SimilartoTUJ1)
AlternateNames
  • Tubulinbeta-III
  • tubulin,beta3
  • tubulin,beta4
BackgroundInformationTubulinisoneofseveralmembersofasmallfamilyofglobularproteins.Themostcommonmembersofthetubulinfamilyareα-tubulinandβ-tubulin,theproteinswhichmakesupmicrotubules.Eachhasamolecularweightofapproximately55kDa.Microtubulesareassembledfromdimersofα-andβ-tubulin.Thesesubunitsareslightlyacidicwithanisoelectricpointbetween5.2and5.8.Tubulinwaslongthoughttobespecifictoeukaryotes.Recently,however,theprokaryoticcelldivisionproteinFtsZwasshowntobeevolutionarilyrelatedtotubulin.
ProductInformation
FormatAscites
Control
  • Positivecontrol:Braintissueorneuronsofanytype.
    Negativecontrol:NonneuraltissueorAstrocytes.
PresentationMousemonoclonalIgG1ascitesinbuffercontainingPBScontaining1%BSAand15mMsodiumazide.
StorageandShippingInformation
StorageConditionsStablefor1yearat-20°Cinundilutedaliquotsfromdateofreceipt.
HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
Applications
ApplicationAnti-TubulinAntibody,betaIIIisoform,C-terminus,cloneTU-20(SimilartoTUJ1)isanantibodyagainstTubulinforuseinELISA,IC,IH,IH(P),IP&WBwithmorethan55productcitations.
KeyApplications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry(Paraffin)
  • Immunoprecipitation
  • WesternBlotting
ApplicationNotesImmunohistochemistry:
Onformalinfixedfrozenorparaffinembeddedtissuesections:1:20to1:100dilutionfromapreviouslot.Hightemperaturecitratebufferantigenretrievalisrecommendedwithparaffinembeddedtissuesections.

Immunoprecipitation:
ApreviouslotofthisantibodywasusedonImmunoprecipitation.

Immunocytochemistry:
1:100to1:500dilutionfromapreviouslotwasusedonfixedcells.

Immunoblotting:
1:1000.Antibodywilldetectasinglebandat50kDacorrespondingtoclassIIIbeta-tubulin.

ELISA:
ApreviouslotofthisantibodywasusedonELISA.

Optimalworkingdilutionmustbedeterminedbytheenduser.
BIOLOGicalInformation
ImmunogenAsyntheticpeptidecorrespondingtoaminoacids443-450(ESEAQGPK)ofhumanclassIIIbeta-tubulinconjugatedtoKLH.
EpitopeC-terminus
CloneTU-20(SimilartoTUJ1)
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostMouse
SpecificityTheantibodyreactswiththeC-terminusoftheneuronspecificbetaIIIisoformoftubulin.Doesnotidentifybetatubulinfoundinglialcells.
IsotypeIgG1
SpeciesReactivity
  • Avian
  • Bovine
  • Human
  • Mouse
  • Monkey
  • Pig
  • Rat
  • Sheep
SpeciesReactivityNoteMouse.Expectedtocross-reactwithAvian,Porcine(Pig),Rat,Bovine,Sheep,Human,Monkey.Reactivitywithotherspecieshasnotbeenconfirmed.
AntibodyTypeMonoclonalAntibody
EntrezGeneNumber
GeneSymbol
  • TUBB3
  • TUBB4
  • beta-4
  • MC1R
PurificationMethodUnpurified
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:Q13509#Tubulinisthemajorconstituentofmicrotubules.ItbindstwomolesofGTP,oneatanexchangeablesiteonthebetachainandoneatanon-exchangeablesiteonthealpha-chain.
SIZE:450aminoacids;50433Da
SUBUNIT:Dimerofalphaandbetachains.
DOMAIN:SwissProt:Q13509ThehighlyacidicC-terminalregionmaybindcationssuchascalcium.
SIMILARITY:Belongstothetubulinfamily.
MolecularWeight55kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。