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当前位置: 首页 > 产品中心 > Functional_antibody > 密理博/AB5829 |抗HuC抗体/AB5829/100µ;G
商品详细密理博/AB5829 |抗HuC抗体/AB5829/100µ;G
密理博/AB5829 |抗HuC抗体/AB5829/100µ;G
密理博/AB5829 |抗HuC抗体/AB5829/100µ;G
商品编号: AB5829
品牌: 密理博
市场价: ¥6980.00
美元价: 4188.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
Catalogue NumberAB5829
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-HuC Antibody
Product Information
FormatAffinity Purified
PresentationAffinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Storage and Shipping Information
Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Applications
ApplicationAnti-HuC Antibody is an antibody against HuC for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern blot: 1:200-1:1,000 using ECL.

Immunocytochemistry: 1:200-1:1,000.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide, amino acids 20-36 of HuC.
HostRabbit
SpecificityRecognizes HuC. The molecular weight of the protein is 38,862 Daltons.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryA member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Gene Symbol
  • ELAVL3
  • PLE21
  • HUCL
  • MGC20653
  • HUC
  • HuC
  • DKFZp547J036
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q14576 # May be involved in neuronal differentiation and maintenance.
SIZE: 367 amino acids; 39547 Da
TISSUE SPECIFICITY: Brain specific.
SIMILARITY: SwissProt: Q14576 ## Belongs to the RRM elav family. & Contains 3 RRM (RNA recognition motif) domains.
Physicochemical Information
Dimensions
Materials Information
Materials Information
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。