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当前位置: 首页 > 产品中心 > Functional_antibody > 密理博/AB5708 |抗HES-5抗体/AB5708/100µ;G
商品详细密理博/AB5708 |抗HES-5抗体/AB5708/100µ;G
密理博/AB5708 |抗HES-5抗体/AB5708/100µ;G
密理博/AB5708 |抗HES-5抗体/AB5708/100µ;G
商品编号: AB5708
品牌: 密理博
市场价: ¥6580.00
美元价: 3948.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberAB5708
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-HES-5Antibody
AlternateNames
  • Hairy5
BackgroundInformationHES-5isamammalianbasichelix-loop-helixfactorthathasadistantsequencehomologytotheproductoftheDrosophilapair-rulegenehairy.HES-5mRNAispresentexclusivelyinthedevelopingnervoussystem,butitsleveldecreasesasneuraldifferentiationproceeds.
ProductInformation
FormatAffinityPurified
PresentationAffinitypurifiedimmunoglobulin.Precipitatedantibodyinasolutionof50%saturatedammoniumsulfateandPBScontainingnopreservatives.
StorageandShippingInformation
StorageConditionsMaintainunopenedvialat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.

PREPARATIONANDUSE:

Toreconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammoniumsulfate/PBSsolution:10μLofresidualammoniumsulfatesolutionwillnotaffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur.

ResuspendtheantibodypelletinasuitableBIOLOGicalbuffersuchasPBSorTBS(pH7.3-7.5)toafinalconcentrationof1.0mg/mL.Forexample,toachievea1.0mg/mLconcentrationwith50μgofprecipitatedantibody,theamountofbufferneededwouldbe50μL.

Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25°Cpriortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses.

Therehydratedantibodysolutionscanbestoredundilutedat2-8°Cfor2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8°C.

Forstorageat-20°C,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.

Forlong-termstorageat-70°C,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.
Applications
ApplicationAnti-HES-5AntibodydetectslevelofHES-5&hasbeenpublished&validatedforuseinWB,IH.
KeyApplications
  • WesternBlotting
  • Immunohistochemistry
ApplicationNotesWesternblot:1:200-1:1,000usingECL.Detectsa28kDabandinembryonalRN46aratcellline{http://www.jneurosci.org/cgi/reprint/23/25/8788.pdf}



Immunohistochemistry:1:200-1:1,000.

Optimalworkingdilutionsmustbedeterminedbytheenduser.
BiologicalInformation
ImmunogenSyntheticpeptide,aminoacids83-95ofmouseHES-5.
HostRabbit
SpecificityRecognizesHES-5(Hairyfamily),aHelix-loop-helixclassoftranscriptionfactor.
SpeciesReactivity
  • Human
  • Mouse
AntibodyTypePolyclonalAntibody
EntrezGeneNumber
GeneSymbol
  • HES5
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:Q5TA89#TranscriptionalrepressorofgenesthatrequireabHLHproteinfortheirtranscription(Bysimilarity).
SIZE:166aminoacids;18226Da
SUBUNIT:Transcriptionrepressionrequiresformationofacomplexwithacorepressorprotein(Groucho)(Bysimilarity).
SUBCELLULARLOCATION:Nucleus(Bysimilarity).
TISSUESPECIFICITY:Expressedinfetalheartandbraintumors.
DOMAIN:SwissProt:Q5TA89Hasaparticulartypeofbasicdomain(presenceofahelix-interruptingproline)thatbindstotheN-box(CACNAG),ratherthanthecanonicalE-box(CANNTG)(Bysimilarity).&TheC-terminalWRPWmotifisatranscriptionalrepressiondomainnecessaryfortheinteractionwithGroucho,atranscriptionalcorepressorrecruitedtospecifictargetDNAbyHairy-relatedproteins(Bysimilarity).
SIMILARITY:Contains1basichelix-loop-helix(bHLH)domain.&Contains1Orangedomain.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。