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当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/AB5688 | Anti-NeuroD 2 Antibody/AB5688/100 µg
商品详细Millipore/AB5688 | Anti-NeuroD 2 Antibody/AB5688/100 µg
Millipore/AB5688 | Anti-NeuroD 2 Antibody/AB5688/100 µg
Millipore/AB5688 | Anti-NeuroD 2 Antibody/AB5688/100 µg
商品编号: AB5688
品牌: 密理博
市场价: ¥7600.00
美元价: 4560.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberAB5688
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-NeuroD2Antibody
AlternateNames
  • NDRF
  • NeuroD-relatedFactor
  • NeurogenicDifferentiationFactor2
  • Ndr2
  • BrainbHLHProteinKW8
BackgroundInformationNeurogenicdifferentiation2,alsoknownasNEUROD2,isahumangene.ThisgeneencodesamemberoftheneuroDfamilyofneurogenicbasichelix-loop-helix(bHLH)proteins.Expressionofthisgenecaninducetranscriptionfromneuron-specificpromoters,suchastheGAP-43promoter,whichcontainaspecificDNAsequenceknownasanE-box.Theproductofthehumangenecaninduceneurogenicdifferentiationinnon-neuronalcellsinXenopusembryos,andisthoughttoplayaroleinthedeterminationandmaintenanceofneuronalcellfates.DiseasesassociatedwithNEUROD2aremotordeficits,ataxia,andseizures.NEUROD2expressionisobservedmainlyinendovascularinvasivecells.
ProductInformation
FormatAffinityPurified
Control
  • Cerebellumtissue
PresentationAffinitypurifiedimmunoglobulin.Precipitatedantibodyinasolutionof50%saturatedammoniumsulfateandPBScontainingnopreservatives.
StorageandShippingInformation
StorageConditionsMaintainunopenedvialat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.

Theantibodymustbe"rehydrated"priortouse:
Torehydrate/reconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammonium
sulfate/PBSsolution:10mLofresidualammoniumsulfatesolutionwillnoteffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur.

ResuspendtheantibodypelletinanysuitableBIOLOGicalbuffer,standardPBSorTBS(pH7.3-7.5)aretypical.Volumesrequiredarenotcriticalbutitissuggestedthatthefinalantibodyconcentrationbebetween0.1mg/mLand1.0mg/mL.Forexample,toachievea1mg/mLconcentrationwith50mgofprecipitatedantibody,theamountofbufferneededwouldbe50mL.

Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25C°priortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses.

Therehydratedantibodycanbestoredat:
2-8°CTherehydratedantibodysolutionscanbestoredundilutedat2-8C°for2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8C°.

-20°CForstorageat-20°C,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.

-70°CForfreezing,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70C°forupto6months.Avoidrepeatedfreeze/thawcycles.
Applications
ApplicationAnti-NeuroD2AntibodydetectslevelofNeuroD2&hasbeenpublished&validatedforuseinIC,IH,IH(P)&WB.
KeyApplications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry(Paraffin)
  • WesternBlotting
ApplicationNotesImmunohistochemistry:1:200-1:1,000.
Immunocytochemistry:1:500onPC12cells
Westernblot:1:200-1:1,000usingECL.

Optimalworkingdilutionsmustbedeterminedbytheenduser.
BiologicalInformation
ImmunogenSyntheticpeptide,aminoacids23-39ofhumanNeuroD2.
HostRabbit
SpecificityRecognizesNeuroD2(NRDL,KW80),aHelix-loop-helixclassoftranscriptionfactor.
SpeciesReactivity
  • Human
  • Mouse
  • Rat
AntibodyTypePolyclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryThisgeneencodesamemberoftheneuroDfamilyofneurogenicbasichelix-loop-helix(bHLH)proteins.Expressionofthisgenecaninducetranscriptionfromneuron-specificpromoters,suchastheGAP-43promoter,whichcontainaspecificDNAsequenceknownasanE-box.Theproductofthehumangenecaninduceneurogenicdifferentiationinnon-neuronalcellsinXenopusembryos,andisthoughttoplayaroleinthedeterminationandmaintenanceofneuronalcellfates.
GeneSymbol
  • NEUROD2
  • NDRF
  • NeuroD2
  • MGC26304
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:Q15784#Appearstomediateneuronaldifferentiation.
SIZE:382aminoacids;41361Da
SUBUNIT:EfficientDNAbindingrequiresdimerizationwithanotherbHLHprotein.
SUBCELLULARLOCATION:Nucleus(Probable).
SIMILARITY:SwissProt:Q15784##Contains1basichelix-loop-helix(bHLH)domain.
MolecularWeight41kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。