密理博/ECM300 |人纤维连接蛋白ELISA/ECM300/1试剂盒-免疫在线蚂蚁淘旗下平台

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商品详细密理博/ECM300 |人纤维连接蛋白ELISA/ECM300/1试剂盒

密理博/ECM300 |人纤维连接蛋白ELISA/ECM300/1试剂盒

商品编号： ECM300

品牌：密理博

市场价： ¥11720.00

美元价： 7032.00

产地：美国(厂家直采)

公司：

产品分类：夹心法ELISA

公司分类： Sandwich_method_ELISA

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Description
CatalogueNumber	ECM300
BrandFamily	Chemicon®
TradeName	Quantimatrix Chemicon
Description	QuantiMatrixHumanFibronectinELISA
Overview	TheQuantiMatrix™ELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatantsandpurifiedproteinsamples. TheassayisintheformatofacompetitiveinhibitionELISA.Humanfibronectinispre-coatedonthewellswiththeexceptionofrowH,whichservesasareference.Standardsandsamplesaredilutedandpre-incubatedwith polyclonalrabbitantibodytohumanfibronectin.Thepolyclonalantibodybindstofibronectininthestandarddilutions,andinthesample,ifpresent.Themixtureisthentransferredtothehumanfibronectin-coatedplate.Freerabbitanti-humanfibronectinbindstothefibronectinontheplate.Goatanti-rabbitIgGHRPconjugatereactswithboundrabbitanti-fibronectin.WhenHRPsubstrateisadded,abluecolordevelops.Thereactionisstoppedbytheadditionofanacid,changingthecolortoyellow.ThiscolorisquantitatedusinganELISAreader;theintensityofthecolorisinverselyproportionaltotheamountoffibronectinintheoriginalsample.Astandardcurveisconstructedandsamplevaluesareinterpolated.
BackgroundInformation	ThedeterminationoflevelsofextracellularmatrixmoleculesisbecomingmoreimportanttounderstandingalterationsinmatrixfunctionduringdevelopmentandtumOrigenesis.Questionsofmatrixgeneregulationandfunctionrequireasensitivemeansofdeterminingthelevelsofproteinexpressionbycells.TheQuantiMatrix™ELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatantsandpurifiedproteinsamples. TheassayisintheformatofacompetitiveinhibitionELISA.Humanfibronectinispre-coatedonthewellswiththeexceptionofrowH,whichservesasareference.Standardsandsamplesaredilutedandpre-incubatedwithpolyclonalrabbitantibodytohumanfibronectin.Thepolyclonalantibodybindstofibronectininthestandarddilutions,andinthesample,ifpresent.Themixtureisthentransferredtothehumanfibronectin-coatedplate.Freerabbitanti-humanfibronectinbindstothefibronectinontheplate.Goatanti-rabbitIgGHRPconjugatereactswithboundrabbitanti-fibronectin.WhenHRPsubstrateisadded,abluecolordevelops.Thereactionisstoppedbytheadditionofanacid,changingthecolortoyellow.ThiscolorisquantitatedusinganELISAreader;theintensityofthecolorisinverselyproportionaltotheamountoffibronectinintheoriginalsample.Astandardcurveisconstructedandsamplevaluesareinterpolated.

ProductInformation
Components	QuantiMatrixTMFibronectinCoatedStrips(PartNo.60103)-1x96-wellstripplate HumanFibronectinStandard(PartNo.60109)-1lyophilizedbottle;reconstituteaccordingtotheprotocolonpage2 SampleDiluent(PartNo.60095)-1x15mLbottleof1%BSAinPBS+0.01%Thimerosal Goatanti-RabbitIgG-HRPConjugate(QM2)(PartNo.60110)-1bottle;readytouse Rabbitanti-HumanFibronectin(PartNo.60111)-1bottlepre-dilutedpolyclonalantibody;readytouse TMB/ESubstrate(PartNo.60096)-1bottle;readytouse StopSolution(PartNo.60193)-1bottle;(0.5MHClsolution) WashBufferConcentrate(PartNo.60245)-1x100mLbottle;providesfinalconcentrationsof0.05%Tween-20and0.01%Thimerosalwhenaddedto 900mLofdeionizedwaterasdescribedinstep1ofprotocolonpage2.
Detectionmethod	Chromogenic

ProductInformation

Components

QuantiMatrixTMFibronectinCoatedStrips(PartNo.60103)-1x96-wellstripplate
HumanFibronectinStandard(PartNo.60109)-1lyophilizedbottle;reconstituteaccordingtotheprotocolonpage2
SampleDiluent(PartNo.60095)-1x15mLbottleof1%BSAinPBS+0.01%Thimerosal
Goatanti-RabbitIgG-HRPConjugate(QM2)(PartNo.60110)-1bottle;readytouse
Rabbitanti-HumanFibronectin(PartNo.60111)-1bottlepre-dilutedpolyclonalantibody;readytouse
TMB/ESubstrate(PartNo.60096)-1bottle;readytouse
StopSolution(PartNo.60193)-1bottle;(0.5MHClsolution)
WashBufferConcentrate(PartNo.60245)-1x100mLbottle;providesfinalconcentrationsof0.05%Tween-20and0.01%Thimerosalwhenaddedto
900mLofdeionizedwaterasdescribedinstep1ofprotocolonpage2.

Detectionmethod

Chromogenic

StorageandShippingInformation
StorageConditions	Storethekitandallcomponentsat2-8°C. PRECAUTIONS: HumanfibronectinwaspurifiedfromhumanplasmathattestednegativeforHBsAgandHIV.However,allmaterialsshouldbehandledcarefullyandinaccordancewithgoodlaboratorypractices. Theinstructionsprovidedhavebeendesignedtooptimizeperformance.Deviationsfromthesuggestedproceduremayresultinsub-optimalperformanceofthekitandfailuretoproduceaccuratedata. Donotmixorsubstitutereagentsfromdifferentkitlots.

StorageandShippingInformation

StorageConditions

Storethekitandallcomponentsat2-8°C.

PRECAUTIONS:

HumanfibronectinwaspurifiedfromhumanplasmathattestednegativeforHBsAgandHIV.However,allmaterialsshouldbehandledcarefullyandinaccordancewithgoodlaboratorypractices.

Theinstructionsprovidedhavebeendesignedtooptimizeperformance.Deviationsfromthesuggestedproceduremayresultinsub-optimalperformanceofthekitandfailuretoproduceaccuratedata.

Donotmixorsubstitutereagentsfromdifferentkitlots.

Applications
Application	TheQuantiMatrixELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatants&purifiedproteinsamples.
KeyApplications	ELISA

BIOLOGicalInformation
SpeciesReactivity	Human
AnalytesAvailable	Fibronectin
EntrezGeneNumber	NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1
EntrezGeneSummary	Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.
GeneSymbol	FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS FINC
UniProtNumber	P02751
UniProtSummary	FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

品牌介绍

密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。

公司介绍

品牌分类

细胞分析蛋白质样品制备细胞培养与系统蛋白质和酶抗体和化验抗体和化验蛋白质检测与定量生命科学研究抑制剂和生化物质

基因组分析批量和定制抗体药物发现与开发

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