微孔/ECM205 |微孔涂层™ ECM筛选套件，1个。ECM101-ECM105/ECM205/1套
市场价：
¥8700.00
美元价：
5220.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ECM205 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Millicoat™ECMScreeningKit,1ea.ECM101-ECM105 |
| Overview | Celladhesionplaysamajorroleincellularcommunicationandregulation,andisoffundamentalimportanceinthedevelopmentandmaintenanceoftissues.Scientistsarecontinuallyexaminingtheadhesionandmigrationofmanydiversecelltypesonvariousextracellularmatrix(ECM)componentproteins.Millicoat™CellAdhesionStripsareprovidedas128-wellremovablestripsinaplateframeforconvenienceandflexibilityindesigningassays.ThewellsinrowsA-GhavebeencoatedwithahumanECMprotein.RowHofeachstripiscoatedwithBSAwhichservesasanegativeassaycontrol.TheMillicoatTMscreeningkitcontainsindividual96-wellplatesforfibronectin,vitronectin,laminin,collagenIandcollagenIV.Cellsareseededontothecoatedsubstrate.Subsequently,adherentcellsarefixedandstained.RelativeattachmentisdeterminedusingabsorbancereADIngs. |
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| ProductInformation | |
|---|---|
| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maybestoredat2-8°Cinthefoilpouchforatleast3months.Unusedstripsmaybeplacedbackinthepouchforstorage.Ensurethatthedesiccantremainsinthepouch,andthatthepouchissecurelyclosed. |
| Applications | |
|---|---|
| Application | TheMillicoatTMscreeningkitcontainsindividual96-wellplatesforfibronectin,vitronectin,laminin,collagenI&collagenIV. |
| KeyApplications |
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| ApplicationNotes | PROCEDURE: NOTE:Optimalassayperformanceisobtainedusingsubconfluentcellcultures.Thiscanbeachievedbysplittingthecells1to2dayspriortoperformingtheassay. 1.Rehydratethestripswith200mLofPBSperwellforatleast15minutesatroomtemperature.RemovethePBSfromtherehydatedstrips. 2.PrepareasinglecellsUSPension,preferablyusinganon-enzymaticdissociationbuffer.Optimumcelldensitymaybedeterminedbytitrationofthecells.Acommonstartingrangeisbetween1x10E05to1x10E07cells/mL. 3.Add100mLofthedilutedcellsuspensiontoeachwell.Incubatetheplateat37°Cfor1hourinaCO2incubator.Gentlywashtheplate3timeswithPBScontainingCa2+/Mg2+(200mL/well). 4.Add100mL/wellof0.2%crystalvioletin10%ethanoltoeachwell.Incubatefor5minutesatroomtemperature.Removethestainfromthewells.Gentlywashtheplate3timeswithPBS(300mL/well). 5.Add100mLofSolubilizationBuffer(A50/50mixtureof0.1MNaH2PO,pH4.5and50%ethanol)toeachwell.Allowstripstoincubateandgentlyshakeatroomtemperatureuntilthecell-boundstainiscompletelysolubilized;approximately5minutes. 6.Determinetheabsorbancesat540-570nmonamicroplatereader. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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