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Millipore/ECM200 | QCM 3µm内皮细胞迁移测定纤维连接蛋白，比色法/ECM200/1，足够12 ass
Millipore/ECM200 | QCM 3µm内皮细胞迁移测定纤维连接蛋白，比色法/ECM200/1，足够12 ass
市场价：
¥9540.00
美元价：
5724.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ECM200 |
| TradeName |
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| Description | QCM3µmEndothelialCellMigrationAssayFibronectin,Colorimetric |
| Overview | Alsoavailable:CellComb™ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere Introduction Angiogenesisisafundamentalprocessinvolvingthegrowthofnewbloodvesselsfrompre-existingvessels.Itisimportantindevelopmentandwoundhealing,aswellaspathologicdiseasessuchasdiabeticretinopathyandcancer.Duringangiogenesis,endothelialcellsneedtomoveoutofexistingvessels,migrateintonewareas,proliferateandassembleintonewcapillaries.Themigrationofendothelialcellsisregulatedbymanyangiogenicfactorsandanti-angiogenicfactors.Itiscriticalforresearcherstounderstandthemechanismsofendothelialcellmigration. Millipore"s3μmQCM™EndothelialCellMigrationAssay–Fibronectin,ColorimetricprovidesaquickandefficientsystemtostudytheABIlityofcompoundstoinduceorinhibitendothelialcellmigration.Thisassayalsoallowsscreeningofpharmacologicalagents,evaluationofintegrinsorotheradhesionreceptorsresponsIBLeforendothelialcellmigration,analysisofgenefunctionintransfectedcells,anddeterminationofECMproteininvolvementincellmovement. Thisversatileassaypermitscountingofindividualmigratorycells,and,moreimportantly,allowsquantitativeanalysisbyopticaldensity(OD)usingastandardmicroplatereader.Thisconvenientassayallowslargescalescreeningandquantitativecomparisonofmultiplesamplesandincludesindividualmigrationcontrolsforeachsample. |
| MaterialsRequiredbutNotDelivered | 1.PrecisionPipettes:sufficientforaliquotingcells. 2.Harvestingbuffer:EDTAortrypsinbasedcelldetachmentbuffer,orothercelldetachmentformulationsasoptimizedbyindividualinvestigators.Millipore’sready-to-usenon-mammaliandetachmentsolution,Accutase™(Cat.No.SCR005)canalsobeused. 3.Endothelialcells(forexample:HUVECcells). 4.Endotheliumcellculturemediumappropriateforsubjectcells,suchasEGM-2(Endothelialcellgrowthmedia-2) 5.QuenchingMedium:serum-freemedium,suchasDMEM,MEMetccontaining5%BSA.Mustcontaindivalentcations(Mg2+,Ca2+)sufficientforquenchingEDTAinharvestingbuffer. 6.SterilePBSorHBSStowashcells. 7.Distilledwater 8.(Optional)Chemoattractantorpharmacologicalagentaddedtoculturemedium. 9.Lowspeedcentrifugeandtubesforcellharvesting. 10.CO2incubatorappropriateforsubjectcells. 11.Hemocytometerorothermeansofcountingcells. 12.Trypanblueorequivalentviabilitystain. 13.Microplatereader(540-570nmdetection)orspectrophotometer. 14.Sterilecellculturehood 15.(Optional)Graduatedocular(calibrated),orautomatedmethodforcountingstainedcellsonamembrane. 16.Shaker |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | FluorescentColorimetric |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Storekitmaterialsat2°to8°Cforuptotheirexpirationdate.Donotfreeze. |
| BIOLOGicalInformation |
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| PhysicochemicalInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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