4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > 微孔/FC010-100MG |人血浆纤维连接蛋白纯化蛋白/FC010-100MG/100MG
商品详细微孔/FC010-100MG |人血浆纤维连接蛋白纯化蛋白/FC010-100MG/100MG
微孔/FC010-100MG |人血浆纤维连接蛋白纯化蛋白/FC010-100MG/100MG
微孔/FC010-100MG |人血浆纤维连接蛋白纯化蛋白/FC010-100MG/100MG
商品编号: FC010-100MG
品牌: 密理博
市场价: ¥38520.00
美元价: 23112.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberFC010-100MG
ReplacesFC010-250MG
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionHumanPlasmaFibronectinPurifiedProtein
OverviewHumanfibronectin(HFN)issuitableforuseasanattachmentfactorinthepropagationofcellsinvitrowhenusedtocoatcellculturesurfaces,includingplasticware,glassware,andmicrocarrierbeads.
ProductInformation
PresentationLyophilizedfrom150mMNaCl,10mMsodiumphosphate,pH7.5,containingnopreservatives.
StorageandShippingInformation
StorageConditionsMaintainlyophilizedmaterialat-20°Cforupto12monthsfromdateofreceipt.Storethereconstitutedmaterialat2º-8°Cforupto6months.
Applications
KeyApplications
  • CellCulture
ApplicationNotesImmunochemicalstandard

Cellattachmentandproliferationassaysonhumanendothelialcells,humankeratinocytesandhumandermalfibroblasts

SuggestedProcedureforCoatingCellCultureware

1.DeterminetheamountofhFNneededtocoatculturevesselsbymultiplyingthetotalsurfacearea(cm2)bythedesiredconcentration(mg/mL)ofhFN.Recommendedamountis2-10mg/cm2.

2.Reconstitutetheentirevial*ofhFNto1mg/mLusingdistilledordeionizedwaterat37ºC.AddthelyophilizedhFNslowlywithconstantstirring,andavoidexcessivefoaming.Breakupanyclumpsthatmightformagainstthesideofthecontainerwithaglassrodorspatula.SterilefilterthehFNsolutiontoremoveanyremaininginsolublematerial.Storethereconstitutedmaterialat2º-8°Cforupto6months.Forworkingdilutions,dilutethematerialtothedesiredconcentrationwithphysiologicalbuffer.FordeterminingfinalproteinconcentrationsbyOD280,theExtinctioncoefficient=1.3[(mg/mL)xcm]-1).

3.Wetthesurfaceofeachculturevesseltobecoatedwithaminimumamountofsterilebalancedsaltsolution(serumandproteinfree)requiredtocovertheentirearea.

4.IntroducetheproperCO2atmosphere,ifrequired.

5.AddthecalculatedamountofhFNtoeachculturevesselandstoretheremaininghFNat2º-8ºCasindicatedbelow.

6.AllowhFNtoadsorbtothesurfaceofthevesselfor5-20minutes.

7.Removeresidualbalancedsaltsolutionbeforeproceedingwithstandardcellcultureprocedures.



*Note:hFNislyophilizedfrombufferedsalineandtheweightofthematerialisnotonlyoftheproteinitself,butincludestheprotein+salts.Thematerialmaynotbehomogeneouswithregardtoprotein/saltratio.
BIOLOGicalInformation
PurityApproximately95%,asdeterminedbySDS-PAGE.Adoublebandof220kDaispresentunderreducedconditions.HFNispurifiedbyaffinitychromatographyongelatinagarose,followedbychromatographyonheparin-agarose.
SourceHumandonorplasma.
EntrezGeneNumber
EntrezGeneSummaryThisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.
GeneSymbol
  • FN1
  • DKFZp686O13149
  • DKFZp686H0342
  • DKFZp686I1370
  • MSF
  • FN
  • CIG
  • DKFZp686F10164
  • LETS
  • FINC
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity).
SIZE:2386aminoacids;262607Da
SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1.
SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix.
TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage.
PTM:Sulfated.
SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains.
StemCellType
  • HumanEmbryonicStemCells
  • MesenchymalStemCells
  • NeuralStemCells
  • HematopoieticStemCells
  • EpithelialCells
  • PancreaticStemCells
  • InducedPluripotentStemCells
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。