Millipore/AB2047 | Anti-Fibronectin Antibody/AB2047/100 µg
市场价：
¥6780.00
美元价：
4068.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | AB2047 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-FibronectinAntibody |
| BackgroundInformation | Fibronectin(FN)isanextracellularadhesionmoleculeanditisinvolvedinmanycellularprocesses,includingtissuerepair,embryogenesis,bloodclotting,andcellmigration/adhesion. Fibronectinexistsintwomainforms:1)asaninsolubleglycoproteindimerthatservesasalinkerintheECM(extracellularmatrix),and;2)asasolubledisulphidelinkeddimerfoundintheplasma(plasmaFN).Theplasmaformissynthesizedbyhepatocytes,andtheECMformismadebyfibroblasts,chondrocytes,endothelialcells,macrophages,aswellascertainepithelialcells. Fibronectinsometimesservesasageneralcelladhesionmoleculebyanchoringcellstocollagenorproteoglycansubstrates.FNalsocanservetoorganizecellularinteractionwiththeECMbybindingtodifferentcomponentsoftheextracellularmatrixandtomembrane-boundFNreceptorsoncellsurfaces.Theimportanceoffibronectinincellmigrationeventsduringembryogenesishasbeendocumentedinseveralcontexts,e.g.:1)mesodermalcellmigrationduringgastrulationcanbeblockedbyinjectionofArg-Gly-Asp(RGD)tripeptidesthatblockcellularFNreceptors(integrins);2)injectionofanti-FNantibodiesintochickembryosblocksmigrationofprecardiaccellstotheembryonicmidline,and;3)thepatternsofFNdepositionindevelopingvertebratelimbsdeterminesthepatternsofprecartilagecelladhesiontotheECM,therebyspecifyinglimb-specificpatternsofchondrogenesis.{D.Marcey,http://www.clunet.edu/BioDev/omm/fibro/frames/fibrotxt.htm}. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Presentation | ProteinGaffinitypurifiedIgGfractionat1.0mg/mLinliquidPBS(0.01Mphosphate,0.09MNaCl)pH7.2withnopreservatives. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfrozenat-20°Cforupto12monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles. |
| Applications | |
|---|---|
| Application | Anti-FibronectinAntibodydetectslevelofFibronectin&hasbeenpublished&validatedforuseinELISA,IF,RIA,WB,IH(P). |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistry:1:80dilutionforimmunofluorescentstainingoffreshfrozenbovineskinandlivertissues.Acetoneormethyl-carnoyfixationisalsoreactive. Immunohistochemistryonparaffinembeddedtissuesrequireslightfixationin2%PFA,4%formalin(lessthan90minutes),acetoneormethyl-carnoyfixation;traditionalformalinfixationisnotrecommended.AntigenretrievalisHIERcitratebuffer;detectionisviaenhancedenzymaticmethodsonly. Immunoblotting:1:1000of2%deoxycholate+10%SDS,6Mureaextractedbovinecellcultures(Kinsella,2000).Antibodydemonstatestheappropriatetwinbandsat~220kDa. Radioimmunoassay ELISA Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Fibronectinextractedandpurifiedfrombovineplasma. |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | Antibodyreactswithbovinefibronectin,anddemonstratescross-reactivityoflessthan0.1%withbovinecollagenstypesI,III,IVbyRIAat1:10,000dilution. |
| SpeciesReactivity |
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| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined. |
| GeneSymbol |
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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