4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Millipore/17-608 | ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set/17-608/10 assays10 assays per kit, ~4μL per ch
商品详细Millipore/17-608 | ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set/17-608/10 assays10 assays per kit, ~4μL per ch
Millipore/17-608 | ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set/17-608/10 assays10 assays per kit, ~4μL per ch
Millipore/17-608 | ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set/17-608/10 assays10 assays per kit, ~4μL per ch
商品编号: 17-608
品牌: 密理博
市场价: ¥8000.00
美元价: 4800.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumber17-608
BrandFamilyUpstate
TradeName
  • ChIPAb+
  • Upstate
DescriptionChIPAb+HDAC1-ChIPValidatedAntibodyandPrimerSet
OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
TheChIPAb+HDAC1setincludestheHDAC1antibody,thenegativecontrolantibodysupernatant(mouseIgG),andqPCRprimersflankinganSp1bindingsiteinhumanp21(WAF1/CIP1/CDKN1A)promoter,amplifyinga105basepairPCRproduct.TheHDAC1andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHDAC1associatedchromatin.
ProductInformation
FormatCultureSupernatant
Control
  • IncludednegativecontrolantibodysupernatantmouseIgGandcontrolprimersspecificforhumanp21(WAF1/CIP1/CDKN1A)promoter.
PresentationAnti-HDAC1(mousemonoclonalIgG1,supernatant).Onevialcontaining40μLofculturesupernatantwith0.05%sodiumazide.Storeat-20°C.

NegativeChIPControlSupernatant.Onevialcontaining40uLofmouseIgGcontainingsupernatantwith0.05%sodiumazide.Storeat-20°C.

ChIPPrimersp21.Onevialcontaining75μLof5μMofeachprimerspecificforaregionofthehumanp21(WAF1/CIP1/CDKN1A)promoter.Storeat-20°C.
P21Forward:CCCACAGCAGAGGAGAAAGAA
P21Reverse:CTGGAAATCTCTGCCCAGACA
StorageandShippingInformation
StorageConditionsStablefor1yearat-20°Cfromdateofreceipt
Applications
ApplicationThisChIPAb+HDAC1-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
KeyApplications
  • WesternBlotting
  • ChromatinImmunoprecipitation(ChIP)
ApplicationNotesChromatinImmunoprecipitationAnalysis:
SonicatedchromatinpreparedfromuntreatedoractinomycinDtreated(30ng/mL,6hrs.)U2OScells(3X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing4μLofMouseAnti-HDAC1andtheMagnaChIP™G(Cat.#17-611)Kit.ImmunoprecipitationofHDAC1associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimersp21flankingthehumanp21promoterthatcontainsanSp1bindingsite(Pleaseseefigures).
FoldDecreaseisaratioofnormalizedmeanIPquantitiesextractedfromstandardcurvesderivedfrominputsofeachchromatinsample.Aspreviouslypublished,HDAC1associationwiththisregionofthep21promoterdecreasesuponactinomycinDtreatment.

WesternBlotAnalysis:
3T3/A31celllysatewasresolvedbyelectrophoresis,transferredtonitrocelluloseandprobedwithanti-HDAC1(1:2500).Proteinswerevisualizedusingagoatanti-mousesecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem(Pleaseseefigures).
BiologicalInformation
ImmunogenTheHDAC1antibodyismadeagainstapeptidecorrespondingtoaminoacids467-482ofmouseHDAC1.
HostMouse
SpecificityHDAC1
IsotypeIgG1
SpeciesReactivity
  • Human
  • Mouse
AntibodyTypeMonoclonalAntibody
EntrezGeneNumber
GeneSymbol
  • HD1
  • HDAC1
  • RPD3L1
UniProtNumber
MolecularWeight60kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。