Millipore/17-473 | STAR MEK1 ELISA试剂盒/17-473/96分析
市场价：
¥0.00
美元价：
0.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-473 |
| BrandFamily | Upstate |
| TradeName |
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| Description | STARMEK1ELISAKit |
| BackgroundInformation | TheUPSTATEcolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsindenaturedcellextracts.TheMEK1plateiscoatedwithaspecificmousemonoclonalanti-MEK1captureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingMEK1antigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-MEK1antibodytodetectthecapturedMEK1ontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardcurve. |
| MaterialsRequiredbutNotDelivered | 1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettors&tipscapableofaccuratelymeasuring1-1000µL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | 1yearat4°C |
| Applications | |
|---|---|
| Application | ThisSTARMEK1ELISAKitisusedtomeasure&quantifyMEK1levels. |
| KeyApplications |
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| BIOLOGicalInformation | |
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| SpeciesReactivity |
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| AnalytesAvailable |
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| EntrezGeneNumber | |
| EntrezGeneSummary | Theproteinencodedbythisgeneisamemberofthedualspecificityproteinkinasefamily,whichactsasamitogen-activatedprotein(MAP)kinasekinase.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actasanintegrationpointformultiplebiochemicalsignals.ThisproteinkinaseliesupstreamofMAPkinasesandstimulatestheenzymaticactivityofMAPkinasesuponwidevarietyofextra-andintracellularsignals.AsanessentialcomponentofMAPkinasesignaltransductionpathway,thiskinaseisinvolvedinmanycellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q02750#CatalyzestheconcomitantphosphorylationofathreonineandatyrosineresidueinaThr-Glu-TyrsequencelocatedinMAPkinases.ActivatesERK1andERK2MAPkinases. SIZE:393aminoacids;43439Da SUBUNIT:InteractswithMORG1(Bysimilarity).InteractswithYersiniayopJ. PTM:PhosphorylationonSer/ThrbyMAPkinasekinasekinases(RAForMEKK1)regulatespositivelythekinaseactivity.&AcetylationbyYersiniayopJpreventsphosphorylationandactivation,thusblockingtheMAPKsignalingpathway. DISEASE:SwissProt:Q02750#DefectsinMAP2K1areacauseofcardiofaciocutaneoussyndrome(CFCsyndrome)[MIM:115150];alsoknownascardio-facio-cutaneoussyndrome.CFCsyndromeischaracterizedbyadistinctivefacialappearance,heartdefectsandmentalretardation.Heartdefectsincludepulmonicstenosis,atrialseptaldefectsandhypertrophiccardiomyopathy.Someaffectedindividualspresentwithectodermalabnormalitiessuchassparse,friablehair,hyperkeratoticskinlesionsandageneralizedichthyosis-likecondition.TypicalfacialfeaturesaresimilartoNoonansyndrome.Theyincludehighforeheadwithbitemporalconstriction,hypoplasticsupraorbitalridges,downslantingpalpebralfissures,adepressednasalbridge,andposteriorlyangulatedearswithprominenthelices.TheinheritanceofCFCsyndromeisautosomaldominant. SIMILARITY:SwissProt:Q02750##Belongstotheproteinkinasesuperfamily.STESer/Thrproteinkinasefamily.MAPkinasekinasesubfamily.&Contains1proteinkinasedomain. |
| PhysicochemicalInformation | |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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