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Millipore/17-470 | phospho-Met (Tyr1230/Tyr1234/Tyr1235) STAR ELISA Kit/17-470/96 assays
Millipore/17-470 | phospho-Met (Tyr1230/Tyr1234/Tyr1235) STAR ELISA Kit/17-470/96 assays
市场价：
¥0.00
美元价：
0.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-470 |
| BrandFamily | Upstate |
| TradeName |
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| Description | phospho-Met(Tyr1230/Tyr1234/Tyr1235)STARELISAKit |
| BackgroundInformation | TestPrinciple: TheUPSTATEcolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.TheMetplateiscoatedwithaspecificmousemonoclonalanti-Metcaptureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingMetantigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-Met(Tyr1230/Tyr1234/Tyr1235)antibodytodetectthecapturedphospho-Met(Tyr1230/Tyr1234/Tyr1235)ontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardcurve. MetBackground: MetisatyrosinekinasereceptorforHepatocyteGrowthFactor(HGF),thoughttostimulatemultiplecellularprocessesincludingcellproliferation,differentiation,cellmigrationandtumOrigenesis.ChronicstimulationofMetoncancercellsisthoughttoplayaroleinmetastasis. |
| MaterialsRequiredbutNotDelivered | 1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettors&tipscapableofaccuratelymeasuring1-1000µL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater |
| ProductInformation | |
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| Components |
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| Detectionmethod | ChromogenicColorimetric |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | 1yearat4°C |
| BIOLOGicalInformation | |
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| EntrezGeneNumber | |
| EntrezGeneSummary | Theproto-oncogeneMETproductisthehepatocytegrowthfactorreceptorandencodestyrosine-kinaseactivity.Theprimarysinglechainprecursorproteinispost-translationallycleavedtoproducethealphaandbetasubunits,whicharedisulfidelinkedtoformthematurereceptor.VariousmutationsintheMETgeneareassociatedwithpapillaryrenalcarcinoma. |
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| Modifications |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P08581#Receptorforhepatocytegrowthfactorandscatterfactor.Hasatyrosine-proteinkinaseactivity. SIZE:1390aminoacids;155527Da SUBUNIT:Heterodimerformedofanalphachain(50kDa)andabetachain(145kDa)whicharedisulfidelinked.BindsPLXNB1andGRB2.InteractswithSPSB1,SPSB2andSPSB4(Bysimilarity).InteractswithINPP5D/SHIP1.WhenphosphorylatedatTyr-1356,interactswithINPPL1/SHIP2.InteractswithRANBP9andRANBP10. SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. DISEASE:SwissProt:P08581#ActivationofMETafterrearrangementwiththeTPRgeneproducesanoncogenicprotein.&DefectsinMETmaybeassociatedwithgastriccancer.&DefectsinMETareacauseofhepatocellularcarcinoma(HCC)[MIM:114550].&DefectsinMETareacauseofhereditarypapillaryrenalcarcinoma(HPRC)[MIM:605074];alsoknownaspapillaryrenalcellcarcinoma2(RCCP2).HPRCisaformofinheritedkidneycancercharacterizedbyapredispositiontodevelopmultiple,bilateralpapillaryrenaltumors.Thepatternofinheritanceisconsistentwithautosomaldominanttransmissionwithreducedpenetrance.&GeneticvariationsinMETmaybeassociatedwithsusceptibilitytoautismtype1B(AUTS1B)[MIM:608636].Autismisaneurodevelopmentaldisordercharacterizedbydisturbanceinlanguage,perceptionandsocialization.Thedisorderisclassicallydefinedbyatriadoflimitedorabsentverbalcommunication,alackofreciprocalsocialinteractionorresponsiveness,andrestricted,stereotypical,andritualizedpatternsofinterestsandbehavior. SIMILARITY:SwissProt:P08581##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.&Contains3IPT/TIGdomains.&Contains1proteinkinasedomain.&Contains1Semadomain. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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